3D bioprinting of artificial skin substrates
In recent years, there has been intensive research to develop skin substitutes to replace the current method of tissue grafting from donor sites. Various tissue-engineered skin replacements have been developed which are currently available on the market. However, these skin replacements do not re...
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| Format: | Final Year Project |
| Language: | English |
| Published: |
2019
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| Subjects: | |
| Online Access: | http://hdl.handle.net/10356/76751 |
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| Institution: | Nanyang Technological University |
| Language: | English |
| Summary: | In recent years, there has been intensive research to develop skin substitutes to replace the
current method of tissue grafting from donor sites. Various tissue-engineered skin replacements
have been developed which are currently available on the market. However, these skin
replacements do not represent a true, fully functional skin replacement as they each have their
own unique drawbacks. This study aims to develop a fully functional skin model to mimic real
skin tissue as close as possible. This is done by coating a bio-printed hydrogel scaffold with a
recombinant protein which will facilitate cell migration, adhesion and proliferation. The
Human Dermal Fibroblast (HDF) cells seeded onto the scaffold were incubated for up to 14
days and the viability of the cells were characterised via Live/Dead cell staining. Images of the
cells was taken at days 1, 3, 7 and 14 using a microscope. The images depicted positive cell
adhesion and proliferation. A dry sample of the bio-printed hydrogel scaffold was also
characterised using Scanning Electron Microscopy to analyse the pore morphology, size and
density. |
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