Fabrication and characterization of human hair keratin templates

Keratins are major structural proteins that can be found in horns, hooves, nails and human hair. Apart from providing structural support, keratins also act as external protective layer by forming intermediate filaments. With their biocompatibility and intrinsic bioactivity, keratins have been studie...

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Main Author: Chia, You Hong
Other Authors: Ng Kee Woei
Format: Final Year Project
Language:English
Published: 2019
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Online Access:http://hdl.handle.net/10356/76752
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Institution: Nanyang Technological University
Language: English
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spelling sg-ntu-dr.10356-767522023-03-04T15:40:57Z Fabrication and characterization of human hair keratin templates Chia, You Hong Ng Kee Woei School of Materials Science and Engineering DRNTU::Engineering::Materials Keratins are major structural proteins that can be found in horns, hooves, nails and human hair. Apart from providing structural support, keratins also act as external protective layer by forming intermediate filaments. With their biocompatibility and intrinsic bioactivity, keratins have been studies intensively and have been synthesized to obtain biomaterials such as keratin films. Different polymers have been added together with keratin to improve the film properties. In this study, human hair was used as a source to extract keratins. Human hair consists of two main types of proteins: keratin intermediate filament proteins (KIFPs) and keratin-associated proteins (KAPs). Little research has been done on the interactions between KIFP and KAP. Thus, this study was carried out to extract KIFP and KAP separately from human hair. Both proteins were then mixed together at different KIFP/KAP ratios (90/10, 95/5, 99/1, 99.25/0.75, 99.5/0.5, 99.75/0.25, 100/0) and fabricated to obtain films through two different procedures: mixing of KIFP and KAP before dialysis and dialysis of KIFP and KAP separately before mixing them together. From the results, using the first procedure of mixing the proteins together before dialysis was unable to obtain the films which contained KAPs. The second procedure of dialyzing the proteins separately before mixing them together was able to obtain the films for all the different ratios. After obtaining the films, they were characterized using several characterization techniques such as Scanning Electron Microscopy (SEM), Fourier Transform Infrared Spectroscopy (FTIR), Ultraviolet-Visible Spectroscopy (UV-VIS) and Differential Scanning Calorimetry (DSC) to observe the changes in properties of KIFP/KAP films of different ratios. From the results obtained through the characterizations, the properties KIFP/KAP films did not have significant changes within the ratios that was done in this study. This study aims to obtain keratin films by mixing KIFP and KAP in different ratios and to observe changes to the properties of the films with the different ratios, giving insights for future work. Bachelor of Engineering (Materials Engineering) 2019-04-08T13:49:58Z 2019-04-08T13:49:58Z 2019 Final Year Project (FYP) http://hdl.handle.net/10356/76752 en Nanyang Technological University 43 p. application/pdf
institution Nanyang Technological University
building NTU Library
continent Asia
country Singapore
Singapore
content_provider NTU Library
collection DR-NTU
language English
topic DRNTU::Engineering::Materials
spellingShingle DRNTU::Engineering::Materials
Chia, You Hong
Fabrication and characterization of human hair keratin templates
description Keratins are major structural proteins that can be found in horns, hooves, nails and human hair. Apart from providing structural support, keratins also act as external protective layer by forming intermediate filaments. With their biocompatibility and intrinsic bioactivity, keratins have been studies intensively and have been synthesized to obtain biomaterials such as keratin films. Different polymers have been added together with keratin to improve the film properties. In this study, human hair was used as a source to extract keratins. Human hair consists of two main types of proteins: keratin intermediate filament proteins (KIFPs) and keratin-associated proteins (KAPs). Little research has been done on the interactions between KIFP and KAP. Thus, this study was carried out to extract KIFP and KAP separately from human hair. Both proteins were then mixed together at different KIFP/KAP ratios (90/10, 95/5, 99/1, 99.25/0.75, 99.5/0.5, 99.75/0.25, 100/0) and fabricated to obtain films through two different procedures: mixing of KIFP and KAP before dialysis and dialysis of KIFP and KAP separately before mixing them together. From the results, using the first procedure of mixing the proteins together before dialysis was unable to obtain the films which contained KAPs. The second procedure of dialyzing the proteins separately before mixing them together was able to obtain the films for all the different ratios. After obtaining the films, they were characterized using several characterization techniques such as Scanning Electron Microscopy (SEM), Fourier Transform Infrared Spectroscopy (FTIR), Ultraviolet-Visible Spectroscopy (UV-VIS) and Differential Scanning Calorimetry (DSC) to observe the changes in properties of KIFP/KAP films of different ratios. From the results obtained through the characterizations, the properties KIFP/KAP films did not have significant changes within the ratios that was done in this study. This study aims to obtain keratin films by mixing KIFP and KAP in different ratios and to observe changes to the properties of the films with the different ratios, giving insights for future work.
author2 Ng Kee Woei
author_facet Ng Kee Woei
Chia, You Hong
format Final Year Project
author Chia, You Hong
author_sort Chia, You Hong
title Fabrication and characterization of human hair keratin templates
title_short Fabrication and characterization of human hair keratin templates
title_full Fabrication and characterization of human hair keratin templates
title_fullStr Fabrication and characterization of human hair keratin templates
title_full_unstemmed Fabrication and characterization of human hair keratin templates
title_sort fabrication and characterization of human hair keratin templates
publishDate 2019
url http://hdl.handle.net/10356/76752
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