Development of scaffolds for tissue engineering applications

Alginate has been widely used as cell carriers for cell-based therapy applications due to its intrinsic properties, such as non-toxic and non-antigenicity. However, the bioinert behavior of alginate often resulted in poor cell interaction that resulted in poor survivability of the cells. Thus, an ef...

Full description

Saved in:
Bibliographic Details
Main Author: Kurniawan, Grace Jasmine
Other Authors: Dalton Tay Chor Yong
Format: Final Year Project
Language:English
Published: 2019
Subjects:
Online Access:http://hdl.handle.net/10356/77113
Tags: Add Tag
No Tags, Be the first to tag this record!
Institution: Nanyang Technological University
Language: English
id sg-ntu-dr.10356-77113
record_format dspace
spelling sg-ntu-dr.10356-771132023-03-04T15:39:49Z Development of scaffolds for tissue engineering applications Kurniawan, Grace Jasmine Dalton Tay Chor Yong School of Materials Science and Engineering DRNTU::Engineering::Materials::Biomaterials Alginate has been widely used as cell carriers for cell-based therapy applications due to its intrinsic properties, such as non-toxic and non-antigenicity. However, the bioinert behavior of alginate often resulted in poor cell interaction that resulted in poor survivability of the cells. Thus, an effort to increase the bioactivity was carried out by incorporating Adipose Tissue-derived ECM (Ad-ECM). However, poor distribution of Ad-ECM across the alginate capsules was observed. In addition, the presence of Ad-ECM affected the capsule formation, in terms of its size and shape that are crucial in determining the biological function of the capsules. For instanceStudies have shown there is shape- and size-dependant on capsules fabricated towards fibrosis formation. Thus, the alginate/Ad-ECM precursor solution was was homogenized, in which successfully improved the distribution and the capsule formation. In addition, the capsule formation was highly dependent on the ratio of alginate and Ad-ECM. Results showed that a final concentrations of 1% alginate and 0.5% Ad-ECM provided the right viscosity and the formability of spherical capsules. Under 10 kV and 3-cm parameter, a 1,300.68 ± 37.09 μm capsules in diameter size was successfully fabricated, which could minimize the formation of fibrosis upon implantation. Finally, physicochemical studies were carried out and showed that the presence of Ad-ECM successfully improved the stability and mechanical properties of the alginate. However, the presence of Ad-ECM did not prolong the degradation stability of the capsules. Hence, improvement to slower down the degradation process of Ad-ECM is needed or due to its short term stability, the carrier would be more suitable for drug delivery applications. Bachelor of Engineering (Materials Engineering) 2019-05-08T13:55:30Z 2019-05-08T13:55:30Z 2019 Final Year Project (FYP) http://hdl.handle.net/10356/77113 en Nanyang Technological University 42 p. application/pdf
institution Nanyang Technological University
building NTU Library
continent Asia
country Singapore
Singapore
content_provider NTU Library
collection DR-NTU
language English
topic DRNTU::Engineering::Materials::Biomaterials
spellingShingle DRNTU::Engineering::Materials::Biomaterials
Kurniawan, Grace Jasmine
Development of scaffolds for tissue engineering applications
description Alginate has been widely used as cell carriers for cell-based therapy applications due to its intrinsic properties, such as non-toxic and non-antigenicity. However, the bioinert behavior of alginate often resulted in poor cell interaction that resulted in poor survivability of the cells. Thus, an effort to increase the bioactivity was carried out by incorporating Adipose Tissue-derived ECM (Ad-ECM). However, poor distribution of Ad-ECM across the alginate capsules was observed. In addition, the presence of Ad-ECM affected the capsule formation, in terms of its size and shape that are crucial in determining the biological function of the capsules. For instanceStudies have shown there is shape- and size-dependant on capsules fabricated towards fibrosis formation. Thus, the alginate/Ad-ECM precursor solution was was homogenized, in which successfully improved the distribution and the capsule formation. In addition, the capsule formation was highly dependent on the ratio of alginate and Ad-ECM. Results showed that a final concentrations of 1% alginate and 0.5% Ad-ECM provided the right viscosity and the formability of spherical capsules. Under 10 kV and 3-cm parameter, a 1,300.68 ± 37.09 μm capsules in diameter size was successfully fabricated, which could minimize the formation of fibrosis upon implantation. Finally, physicochemical studies were carried out and showed that the presence of Ad-ECM successfully improved the stability and mechanical properties of the alginate. However, the presence of Ad-ECM did not prolong the degradation stability of the capsules. Hence, improvement to slower down the degradation process of Ad-ECM is needed or due to its short term stability, the carrier would be more suitable for drug delivery applications.
author2 Dalton Tay Chor Yong
author_facet Dalton Tay Chor Yong
Kurniawan, Grace Jasmine
format Final Year Project
author Kurniawan, Grace Jasmine
author_sort Kurniawan, Grace Jasmine
title Development of scaffolds for tissue engineering applications
title_short Development of scaffolds for tissue engineering applications
title_full Development of scaffolds for tissue engineering applications
title_fullStr Development of scaffolds for tissue engineering applications
title_full_unstemmed Development of scaffolds for tissue engineering applications
title_sort development of scaffolds for tissue engineering applications
publishDate 2019
url http://hdl.handle.net/10356/77113
_version_ 1759855953609490432