Investigating the contribution of NF-κB2 to tumor proliferation and migration in glioblastoma multiforme
Glioblastoma multiforme (GBM) is the deadliest type of astrocytoma whereby patients do not live over 2 years. The non-canonical nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) signaling was reported to be hyperactivated in some human GBM cells and this is correlated with enhan...
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sg-ntu-dr.10356-771212023-02-28T18:02:34Z Investigating the contribution of NF-κB2 to tumor proliferation and migration in glioblastoma multiforme Goh, Zheng Cong Li Yinghui School of Biological Sciences DRNTU::Science::Biological sciences::Molecular biology Glioblastoma multiforme (GBM) is the deadliest type of astrocytoma whereby patients do not live over 2 years. The non-canonical nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) signaling was reported to be hyperactivated in some human GBM cells and this is correlated with enhanced tumor proliferation and motility. NF-кB2 (p100/p52) is a transcription factor in the non-canonical NF-кB signaling pathway. However, how NF-кB2 contributes to GBM progression is poorly defined. Given that GBM is aggressive and invasive, this study aims to study the role of NF-кB2 in GBM proliferation and migration. This project focuses on characterizing endogenous NF-кB2 levels in different human GBM cell lines, overexpressing and knocking out p52, the active form nfкb2, in NF-кB2low and NF-кB2high cells respectively, and conducting functional assays to study the effects of NF-кB2 on GBM proliferation and migration. Our results show that p52 was successfully overexpressed in NF-кB2low LN18, D247 and LN382 human GBM cell lines, while p52 was knocked out in NF-кB2high LN444 and U87 cells. This study also demonstrates that NF-кB2 promotes GBM proliferation but has minimal effects on GBM migration. This calls for more investigations to confirm the role of non-canonical NF-кB signaling pathway in GBM. Bachelor of Science in Biological Sciences 2019-05-09T06:18:23Z 2019-05-09T06:18:23Z 2019 Final Year Project (FYP) http://hdl.handle.net/10356/77121 en Nanyang Technological University 35 p. application/pdf |
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DRNTU::Science::Biological sciences::Molecular biology Goh, Zheng Cong Investigating the contribution of NF-κB2 to tumor proliferation and migration in glioblastoma multiforme |
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Glioblastoma multiforme (GBM) is the deadliest type of astrocytoma whereby patients do not live over 2 years. The non-canonical nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) signaling was reported to be hyperactivated in some human GBM cells and this is correlated with enhanced tumor proliferation and motility. NF-кB2 (p100/p52) is a transcription factor in the non-canonical NF-кB signaling pathway. However, how NF-кB2 contributes to GBM progression is poorly defined. Given that GBM is aggressive and invasive, this study aims to study the role of NF-кB2 in GBM proliferation and migration. This project focuses on characterizing endogenous NF-кB2 levels in different human GBM cell lines, overexpressing and knocking out p52, the active form nfкb2, in NF-кB2low and NF-кB2high cells respectively, and conducting functional assays to study the effects of NF-кB2 on GBM proliferation and migration. Our results show that p52 was successfully overexpressed in NF-кB2low LN18, D247 and LN382 human GBM cell lines, while p52 was knocked out in NF-кB2high LN444 and U87 cells. This study also demonstrates that NF-кB2 promotes GBM proliferation but has minimal effects on GBM migration. This calls for more investigations to confirm the role of non-canonical NF-кB signaling pathway in GBM. |
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Li Yinghui |
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Li Yinghui Goh, Zheng Cong |
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Final Year Project |
author |
Goh, Zheng Cong |
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Goh, Zheng Cong |
title |
Investigating the contribution of NF-κB2 to tumor proliferation and migration in glioblastoma multiforme |
title_short |
Investigating the contribution of NF-κB2 to tumor proliferation and migration in glioblastoma multiforme |
title_full |
Investigating the contribution of NF-κB2 to tumor proliferation and migration in glioblastoma multiforme |
title_fullStr |
Investigating the contribution of NF-κB2 to tumor proliferation and migration in glioblastoma multiforme |
title_full_unstemmed |
Investigating the contribution of NF-κB2 to tumor proliferation and migration in glioblastoma multiforme |
title_sort |
investigating the contribution of nf-κb2 to tumor proliferation and migration in glioblastoma multiforme |
publishDate |
2019 |
url |
http://hdl.handle.net/10356/77121 |
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1759853130669883392 |