Investigating the contribution of NF-κB2 to tumor proliferation and migration in glioblastoma multiforme

Glioblastoma multiforme (GBM) is the deadliest type of astrocytoma whereby patients do not live over 2 years. The non-canonical nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) signaling was reported to be hyperactivated in some human GBM cells and this is correlated with enhan...

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Main Author: Goh, Zheng Cong
Other Authors: Li Yinghui
Format: Final Year Project
Language:English
Published: 2019
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Online Access:http://hdl.handle.net/10356/77121
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Institution: Nanyang Technological University
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spelling sg-ntu-dr.10356-771212023-02-28T18:02:34Z Investigating the contribution of NF-κB2 to tumor proliferation and migration in glioblastoma multiforme Goh, Zheng Cong Li Yinghui School of Biological Sciences DRNTU::Science::Biological sciences::Molecular biology Glioblastoma multiforme (GBM) is the deadliest type of astrocytoma whereby patients do not live over 2 years. The non-canonical nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) signaling was reported to be hyperactivated in some human GBM cells and this is correlated with enhanced tumor proliferation and motility. NF-кB2 (p100/p52) is a transcription factor in the non-canonical NF-кB signaling pathway. However, how NF-кB2 contributes to GBM progression is poorly defined. Given that GBM is aggressive and invasive, this study aims to study the role of NF-кB2 in GBM proliferation and migration. This project focuses on characterizing endogenous NF-кB2 levels in different human GBM cell lines, overexpressing and knocking out p52, the active form nfкb2, in NF-кB2low and NF-кB2high cells respectively, and conducting functional assays to study the effects of NF-кB2 on GBM proliferation and migration. Our results show that p52 was successfully overexpressed in NF-кB2low LN18, D247 and LN382 human GBM cell lines, while p52 was knocked out in NF-кB2high LN444 and U87 cells. This study also demonstrates that NF-кB2 promotes GBM proliferation but has minimal effects on GBM migration. This calls for more investigations to confirm the role of non-canonical NF-кB signaling pathway in GBM. Bachelor of Science in Biological Sciences 2019-05-09T06:18:23Z 2019-05-09T06:18:23Z 2019 Final Year Project (FYP) http://hdl.handle.net/10356/77121 en Nanyang Technological University 35 p. application/pdf
institution Nanyang Technological University
building NTU Library
continent Asia
country Singapore
Singapore
content_provider NTU Library
collection DR-NTU
language English
topic DRNTU::Science::Biological sciences::Molecular biology
spellingShingle DRNTU::Science::Biological sciences::Molecular biology
Goh, Zheng Cong
Investigating the contribution of NF-κB2 to tumor proliferation and migration in glioblastoma multiforme
description Glioblastoma multiforme (GBM) is the deadliest type of astrocytoma whereby patients do not live over 2 years. The non-canonical nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) signaling was reported to be hyperactivated in some human GBM cells and this is correlated with enhanced tumor proliferation and motility. NF-кB2 (p100/p52) is a transcription factor in the non-canonical NF-кB signaling pathway. However, how NF-кB2 contributes to GBM progression is poorly defined. Given that GBM is aggressive and invasive, this study aims to study the role of NF-кB2 in GBM proliferation and migration. This project focuses on characterizing endogenous NF-кB2 levels in different human GBM cell lines, overexpressing and knocking out p52, the active form nfкb2, in NF-кB2low and NF-кB2high cells respectively, and conducting functional assays to study the effects of NF-кB2 on GBM proliferation and migration. Our results show that p52 was successfully overexpressed in NF-кB2low LN18, D247 and LN382 human GBM cell lines, while p52 was knocked out in NF-кB2high LN444 and U87 cells. This study also demonstrates that NF-кB2 promotes GBM proliferation but has minimal effects on GBM migration. This calls for more investigations to confirm the role of non-canonical NF-кB signaling pathway in GBM.
author2 Li Yinghui
author_facet Li Yinghui
Goh, Zheng Cong
format Final Year Project
author Goh, Zheng Cong
author_sort Goh, Zheng Cong
title Investigating the contribution of NF-κB2 to tumor proliferation and migration in glioblastoma multiforme
title_short Investigating the contribution of NF-κB2 to tumor proliferation and migration in glioblastoma multiforme
title_full Investigating the contribution of NF-κB2 to tumor proliferation and migration in glioblastoma multiforme
title_fullStr Investigating the contribution of NF-κB2 to tumor proliferation and migration in glioblastoma multiforme
title_full_unstemmed Investigating the contribution of NF-κB2 to tumor proliferation and migration in glioblastoma multiforme
title_sort investigating the contribution of nf-κb2 to tumor proliferation and migration in glioblastoma multiforme
publishDate 2019
url http://hdl.handle.net/10356/77121
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