Investigation of the effect of sodium dodecylsulfate (SDS) on cell-detachment using quantitative phase interferometry (QPI) method

• Main Author: Chan, Darren Chun Kit
• Other Authors: Chian Kerm Sin, Sandy
• Format: Final Year Project
• Language: English
• Published: 2019
• Subjects: Engineering::Mechanical engineering
• Online Access: http://hdl.handle.net/10356/78746
• Institution: Nanyang Technological University

Sodium Dodecylsulfate (SDS) is a widely used surfactant known for its effectiveness in decellularization. In this report, utilising Quantitative Phase Imaging (QPI) methods to observe changes within L929 fibroblast cells to quantify the steps from adding surfactant to cell detachment. The objective is to observe the behaviour of cells from introduction of SDS leading to cell detachment. Changes in shape, size, height and even volume can be quantified by QPI. The study and tests showed that SDS in all cases detaches the cell and removes all cell materials from surface. The process begins with bloating of the cell followed by bursting due to SDS action on cell membranes. The experiments show that SDS is an ideal surfactant for removing cells completely and in this report are the results of the investigation on the actions and steps leading up to cell detachment.