Parasitic factors and gametocytogenesis

• Main Author: Ang, Sheng Zhi
• Other Authors: Peter Rainer Preiser
• Format: Final Year Project
• Language: English
• Published: 2019
• Subjects: Science::Biological sciences
• Online Access: http://hdl.handle.net/10356/78858
• Institution: Nanyang Technological University

It has been shown that gametocytogenesis of malaria can be affected by environmental changes. However, the actual reason behind this occurrence is still unknown. Therefore, to study the mechanism behind this phenomenon, we have selected three possible genes that could be involved in detecting or transducing these extracellular changes to the parasite within the infected erythrocyte. These three genes include a heat shock protein 40, a serine/threonine kinase and an exported protein with unknown function. To investigate their involvement, the wildtype gene must be modified so that their expression can be controlled. In this paper, we attempted to assemble a linear plasmid via Gibson Assembly which will be later used to transfect the parasites. One of the plasmids had been successfully constructed. However, we realised that the success rate in joining the right-hand region (RHR) onto the plasmid was very low and had hypothesized that it might be due to the restriction site (I-SceI) that was used for the addition of RHR.