Metabolite-enabled mutualistic interaction between Shewanella oneidensis and Escherichia coli in a co-culture using an electrode as electron acceptor

Mutualistic interactions in planktonic microbial communities have been extensively studied. However, our understanding on mutualistic communities consisting of co-existing planktonic cells and biofilms is limited. Here, we report a planktonic cells-biofilm mutualistic system established by the ferme...

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Main Authors: Wang, Victor Bochuan, Sivakumar, Krishnakumar, Yang, Liang, Zhang, Qichun, Kjelleberg, Staffan, Loo, Say Chye Joachim, Cao, Bin
Other Authors: School of Civil and Environmental Engineering
Format: Article
Language:English
Published: 2015
Online Access:https://hdl.handle.net/10356/79270
http://hdl.handle.net/10220/38735
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Institution: Nanyang Technological University
Language: English
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spelling sg-ntu-dr.10356-792702022-02-16T16:29:09Z Metabolite-enabled mutualistic interaction between Shewanella oneidensis and Escherichia coli in a co-culture using an electrode as electron acceptor Wang, Victor Bochuan Sivakumar, Krishnakumar Yang, Liang Zhang, Qichun Kjelleberg, Staffan Loo, Say Chye Joachim Cao, Bin School of Civil and Environmental Engineering School of Materials Science & Engineering School of Biological Sciences Singapore Centre for Environmental Life Sciences Engineering Mutualistic interactions in planktonic microbial communities have been extensively studied. However, our understanding on mutualistic communities consisting of co-existing planktonic cells and biofilms is limited. Here, we report a planktonic cells-biofilm mutualistic system established by the fermentative bacterium Escherichia coli and the dissimilatory metal-reducing bacterium Shewanella oneidensis in a bioelectrochemical device, where planktonic cells in the anode media interact with the biofilms on the electrode. Our results show that the transfer of formate is the key mechanism in this mutualistic system. More importantly, we demonstrate that the relative distribution of E. coli and S. oneidensis in the liquid media and biofilm is likely driven by their metabolic functions towards an optimum communal metabolism in the bioelectrochemical device. RNA sequencing-based transcriptomic analyses of the interacting organisms in the mutualistic system potentially reveal differential expression of genes involved in extracellular electron transfer pathways in both species in the planktonic cultures and biofilms. Published version 2015-09-23T04:06:51Z 2019-12-06T13:21:17Z 2015-09-23T04:06:51Z 2019-12-06T13:21:17Z 2015 2015 Journal Article Wang, V. B., Sivakumar, K., Yang, L., Zhang, Q., Kjelleberg, S., Loo, S. C. J., et al. (2015). Metabolite-enabled mutualistic interaction between Shewanella oneidensis and Escherichia coli in a co-culture using an electrode as electron acceptor. Scientific Reports, 5, 11222-. 2045-2322 https://hdl.handle.net/10356/79270 http://hdl.handle.net/10220/38735 10.1038/srep11222 26061569 en Scientific Reports This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ application/pdf
institution Nanyang Technological University
building NTU Library
continent Asia
country Singapore
Singapore
content_provider NTU Library
collection DR-NTU
language English
description Mutualistic interactions in planktonic microbial communities have been extensively studied. However, our understanding on mutualistic communities consisting of co-existing planktonic cells and biofilms is limited. Here, we report a planktonic cells-biofilm mutualistic system established by the fermentative bacterium Escherichia coli and the dissimilatory metal-reducing bacterium Shewanella oneidensis in a bioelectrochemical device, where planktonic cells in the anode media interact with the biofilms on the electrode. Our results show that the transfer of formate is the key mechanism in this mutualistic system. More importantly, we demonstrate that the relative distribution of E. coli and S. oneidensis in the liquid media and biofilm is likely driven by their metabolic functions towards an optimum communal metabolism in the bioelectrochemical device. RNA sequencing-based transcriptomic analyses of the interacting organisms in the mutualistic system potentially reveal differential expression of genes involved in extracellular electron transfer pathways in both species in the planktonic cultures and biofilms.
author2 School of Civil and Environmental Engineering
author_facet School of Civil and Environmental Engineering
Wang, Victor Bochuan
Sivakumar, Krishnakumar
Yang, Liang
Zhang, Qichun
Kjelleberg, Staffan
Loo, Say Chye Joachim
Cao, Bin
format Article
author Wang, Victor Bochuan
Sivakumar, Krishnakumar
Yang, Liang
Zhang, Qichun
Kjelleberg, Staffan
Loo, Say Chye Joachim
Cao, Bin
spellingShingle Wang, Victor Bochuan
Sivakumar, Krishnakumar
Yang, Liang
Zhang, Qichun
Kjelleberg, Staffan
Loo, Say Chye Joachim
Cao, Bin
Metabolite-enabled mutualistic interaction between Shewanella oneidensis and Escherichia coli in a co-culture using an electrode as electron acceptor
author_sort Wang, Victor Bochuan
title Metabolite-enabled mutualistic interaction between Shewanella oneidensis and Escherichia coli in a co-culture using an electrode as electron acceptor
title_short Metabolite-enabled mutualistic interaction between Shewanella oneidensis and Escherichia coli in a co-culture using an electrode as electron acceptor
title_full Metabolite-enabled mutualistic interaction between Shewanella oneidensis and Escherichia coli in a co-culture using an electrode as electron acceptor
title_fullStr Metabolite-enabled mutualistic interaction between Shewanella oneidensis and Escherichia coli in a co-culture using an electrode as electron acceptor
title_full_unstemmed Metabolite-enabled mutualistic interaction between Shewanella oneidensis and Escherichia coli in a co-culture using an electrode as electron acceptor
title_sort metabolite-enabled mutualistic interaction between shewanella oneidensis and escherichia coli in a co-culture using an electrode as electron acceptor
publishDate 2015
url https://hdl.handle.net/10356/79270
http://hdl.handle.net/10220/38735
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