Metabolite-enabled mutualistic interaction between Shewanella oneidensis and Escherichia coli in a co-culture using an electrode as electron acceptor
Mutualistic interactions in planktonic microbial communities have been extensively studied. However, our understanding on mutualistic communities consisting of co-existing planktonic cells and biofilms is limited. Here, we report a planktonic cells-biofilm mutualistic system established by the ferme...
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sg-ntu-dr.10356-792702022-02-16T16:29:09Z Metabolite-enabled mutualistic interaction between Shewanella oneidensis and Escherichia coli in a co-culture using an electrode as electron acceptor Wang, Victor Bochuan Sivakumar, Krishnakumar Yang, Liang Zhang, Qichun Kjelleberg, Staffan Loo, Say Chye Joachim Cao, Bin School of Civil and Environmental Engineering School of Materials Science & Engineering School of Biological Sciences Singapore Centre for Environmental Life Sciences Engineering Mutualistic interactions in planktonic microbial communities have been extensively studied. However, our understanding on mutualistic communities consisting of co-existing planktonic cells and biofilms is limited. Here, we report a planktonic cells-biofilm mutualistic system established by the fermentative bacterium Escherichia coli and the dissimilatory metal-reducing bacterium Shewanella oneidensis in a bioelectrochemical device, where planktonic cells in the anode media interact with the biofilms on the electrode. Our results show that the transfer of formate is the key mechanism in this mutualistic system. More importantly, we demonstrate that the relative distribution of E. coli and S. oneidensis in the liquid media and biofilm is likely driven by their metabolic functions towards an optimum communal metabolism in the bioelectrochemical device. RNA sequencing-based transcriptomic analyses of the interacting organisms in the mutualistic system potentially reveal differential expression of genes involved in extracellular electron transfer pathways in both species in the planktonic cultures and biofilms. Published version 2015-09-23T04:06:51Z 2019-12-06T13:21:17Z 2015-09-23T04:06:51Z 2019-12-06T13:21:17Z 2015 2015 Journal Article Wang, V. B., Sivakumar, K., Yang, L., Zhang, Q., Kjelleberg, S., Loo, S. C. J., et al. (2015). Metabolite-enabled mutualistic interaction between Shewanella oneidensis and Escherichia coli in a co-culture using an electrode as electron acceptor. Scientific Reports, 5, 11222-. 2045-2322 https://hdl.handle.net/10356/79270 http://hdl.handle.net/10220/38735 10.1038/srep11222 26061569 en Scientific Reports This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ application/pdf |
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Mutualistic interactions in planktonic microbial communities have been extensively studied. However, our understanding on mutualistic communities consisting of co-existing planktonic cells and biofilms is limited. Here, we report a planktonic cells-biofilm mutualistic system established by the fermentative bacterium Escherichia coli and the dissimilatory metal-reducing bacterium Shewanella oneidensis in a bioelectrochemical device, where planktonic cells in the anode media interact with the biofilms on the electrode. Our results show that the transfer of formate is the key mechanism in this mutualistic system. More importantly, we demonstrate that the relative distribution of E. coli and S. oneidensis in the liquid media and biofilm is likely driven by their metabolic functions towards an optimum communal metabolism in the bioelectrochemical device. RNA sequencing-based transcriptomic analyses of the interacting organisms in the mutualistic system potentially reveal differential expression of genes involved in extracellular electron transfer pathways in both species in the planktonic cultures and biofilms. |
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School of Civil and Environmental Engineering |
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School of Civil and Environmental Engineering Wang, Victor Bochuan Sivakumar, Krishnakumar Yang, Liang Zhang, Qichun Kjelleberg, Staffan Loo, Say Chye Joachim Cao, Bin |
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Wang, Victor Bochuan Sivakumar, Krishnakumar Yang, Liang Zhang, Qichun Kjelleberg, Staffan Loo, Say Chye Joachim Cao, Bin |
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Wang, Victor Bochuan Sivakumar, Krishnakumar Yang, Liang Zhang, Qichun Kjelleberg, Staffan Loo, Say Chye Joachim Cao, Bin Metabolite-enabled mutualistic interaction between Shewanella oneidensis and Escherichia coli in a co-culture using an electrode as electron acceptor |
author_sort |
Wang, Victor Bochuan |
title |
Metabolite-enabled mutualistic interaction between Shewanella oneidensis and Escherichia coli in a co-culture using an electrode as electron acceptor |
title_short |
Metabolite-enabled mutualistic interaction between Shewanella oneidensis and Escherichia coli in a co-culture using an electrode as electron acceptor |
title_full |
Metabolite-enabled mutualistic interaction between Shewanella oneidensis and Escherichia coli in a co-culture using an electrode as electron acceptor |
title_fullStr |
Metabolite-enabled mutualistic interaction between Shewanella oneidensis and Escherichia coli in a co-culture using an electrode as electron acceptor |
title_full_unstemmed |
Metabolite-enabled mutualistic interaction between Shewanella oneidensis and Escherichia coli in a co-culture using an electrode as electron acceptor |
title_sort |
metabolite-enabled mutualistic interaction between shewanella oneidensis and escherichia coli in a co-culture using an electrode as electron acceptor |
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2015 |
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https://hdl.handle.net/10356/79270 http://hdl.handle.net/10220/38735 |
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1725985714158436352 |