Dose-response algorithms for water-borne Pseudomonas aeruginosa folliculitis

We developed two dose–response algorithms for P. aeruginosa pool folliculitis using bacterial and lesion density estimates, associated with undetectable, significant, and almost certain folliculitis. Literature data were fitted to Furumoto & Mickey's equations, developed for plant epidermis...

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Main Authors: Roser, D. J., Van Den Akker, B., Boase, S., Haas, C. N., Ashbolt, N. J., Rice, S. A.
Other Authors: School of Biological Sciences
Format: Article
Language:English
Published: 2014
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Online Access:https://hdl.handle.net/10356/79420
http://hdl.handle.net/10220/24363
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Institution: Nanyang Technological University
Language: English
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spelling sg-ntu-dr.10356-794202023-02-28T16:59:31Z Dose-response algorithms for water-borne Pseudomonas aeruginosa folliculitis Roser, D. J. Van Den Akker, B. Boase, S. Haas, C. N. Ashbolt, N. J. Rice, S. A. School of Biological Sciences DRNTU::Engineering::Environmental engineering DRNTU::Science::Biological sciences We developed two dose–response algorithms for P. aeruginosa pool folliculitis using bacterial and lesion density estimates, associated with undetectable, significant, and almost certain folliculitis. Literature data were fitted to Furumoto & Mickey's equations, developed for plant epidermis-invading pathogens: N l = A ln(1 + BC) (log-linear model); Pinf = 1−e(−r c C) (exponential model), where A and B are 2.51644 × 107 lesions/m2 and 2.28011 × 10−11 c.f.u./ml P. aeruginosa, respectively; C = pathogen density (c.f.u./ml), N l = folliculitis lesions/m2, Pinf = probability of infection, and r C = 4·3 × 10−7 c.f.u./ml P. aeruginosa. Outbreak data indicates these algorithms apply to exposure durations of 41 ± 25 min. Typical water quality benchmarks (≈10−2 c.f.u./ml) appear conservative but still useful as the literature indicated repeated detection likely implies unstable control barriers and bacterial bloom potential. In future, culture-based outbreak testing should be supplemented with quantitative polymerase chain reaction and organic carbon assays, and quantification of folliculitis aetiology to better understand P. aeruginosa risks. Published version 2014-12-09T03:49:51Z 2019-12-06T13:24:50Z 2014-12-09T03:49:51Z 2019-12-06T13:24:50Z 2014 2014 Journal Article Roser, D. J., Van Den Akker, B., Boase, S., Haas, C. N., Ashbolt, N. J., & Rice, S. A. Dose–response algorithms for water-borne Pseudomonas aeruginosa folliculitis. Epidemiology and infection,143(7), 1525-1537. https://hdl.handle.net/10356/79420 http://hdl.handle.net/10220/24363 10.1017/S0950268814002532 en Epidemiology and infection © 2014 Cambridge University Press. This paper was published in Epidemiology and Infection and is made available as an electronic reprint (preprint) with permission of Cambridge University Press. The paper can be found at the following official DOI: [http://dx.doi.org/10.1017/S0950268814002532].  One print or electronic copy may be made for personal use only. Systematic or multiple reproduction, distribution to multiple locations via electronic or other means, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper is prohibited and is subject to penalties under law. application/pdf
institution Nanyang Technological University
building NTU Library
continent Asia
country Singapore
Singapore
content_provider NTU Library
collection DR-NTU
language English
topic DRNTU::Engineering::Environmental engineering
DRNTU::Science::Biological sciences
spellingShingle DRNTU::Engineering::Environmental engineering
DRNTU::Science::Biological sciences
Roser, D. J.
Van Den Akker, B.
Boase, S.
Haas, C. N.
Ashbolt, N. J.
Rice, S. A.
Dose-response algorithms for water-borne Pseudomonas aeruginosa folliculitis
description We developed two dose–response algorithms for P. aeruginosa pool folliculitis using bacterial and lesion density estimates, associated with undetectable, significant, and almost certain folliculitis. Literature data were fitted to Furumoto & Mickey's equations, developed for plant epidermis-invading pathogens: N l = A ln(1 + BC) (log-linear model); Pinf = 1−e(−r c C) (exponential model), where A and B are 2.51644 × 107 lesions/m2 and 2.28011 × 10−11 c.f.u./ml P. aeruginosa, respectively; C = pathogen density (c.f.u./ml), N l = folliculitis lesions/m2, Pinf = probability of infection, and r C = 4·3 × 10−7 c.f.u./ml P. aeruginosa. Outbreak data indicates these algorithms apply to exposure durations of 41 ± 25 min. Typical water quality benchmarks (≈10−2 c.f.u./ml) appear conservative but still useful as the literature indicated repeated detection likely implies unstable control barriers and bacterial bloom potential. In future, culture-based outbreak testing should be supplemented with quantitative polymerase chain reaction and organic carbon assays, and quantification of folliculitis aetiology to better understand P. aeruginosa risks.
author2 School of Biological Sciences
author_facet School of Biological Sciences
Roser, D. J.
Van Den Akker, B.
Boase, S.
Haas, C. N.
Ashbolt, N. J.
Rice, S. A.
format Article
author Roser, D. J.
Van Den Akker, B.
Boase, S.
Haas, C. N.
Ashbolt, N. J.
Rice, S. A.
author_sort Roser, D. J.
title Dose-response algorithms for water-borne Pseudomonas aeruginosa folliculitis
title_short Dose-response algorithms for water-borne Pseudomonas aeruginosa folliculitis
title_full Dose-response algorithms for water-borne Pseudomonas aeruginosa folliculitis
title_fullStr Dose-response algorithms for water-borne Pseudomonas aeruginosa folliculitis
title_full_unstemmed Dose-response algorithms for water-borne Pseudomonas aeruginosa folliculitis
title_sort dose-response algorithms for water-borne pseudomonas aeruginosa folliculitis
publishDate 2014
url https://hdl.handle.net/10356/79420
http://hdl.handle.net/10220/24363
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