Adhesion, proliferation, and gene expression profile of human umbilical vein endothelial cells cultured on bilayered polyelectrolyte coatings composed of glycosaminoglycans

This study characterized human umbilical vein endothelial cell HUVEC adhesion, proliferation, and gene expression on bilayered polyelectrolyte coatings composed of an outermost layer of glycosaminoglycans hyaluronan, heparin, or chondroitin sulfate, with an underlying layer of poly-l-lysine or chito...

Full description

Saved in:
Bibliographic Details
Main Authors: Boey, Freddy Yin Chiang, Heng, Boon Chin, Bezerra, Paula Porto, Meng, Qing Rui, Chin, Desmond Wai-Loon, Koh, Li Buay, Li, Hai, Zhang, Hua, Preiser, Peter Rainer, Venkatraman, Subbu S.
Other Authors: School of Materials Science & Engineering
Format: Article
Language:English
Published: 2012
Subjects:
Online Access:https://hdl.handle.net/10356/79974
http://hdl.handle.net/10220/8709
Tags: Add Tag
No Tags, Be the first to tag this record!
Institution: Nanyang Technological University
Language: English
id sg-ntu-dr.10356-79974
record_format dspace
spelling sg-ntu-dr.10356-799742023-02-28T17:06:43Z Adhesion, proliferation, and gene expression profile of human umbilical vein endothelial cells cultured on bilayered polyelectrolyte coatings composed of glycosaminoglycans Boey, Freddy Yin Chiang Heng, Boon Chin Bezerra, Paula Porto Meng, Qing Rui Chin, Desmond Wai-Loon Koh, Li Buay Li, Hai Zhang, Hua Preiser, Peter Rainer Venkatraman, Subbu S. School of Materials Science & Engineering DRNTU::Science::Medicine::Biomedical engineering This study characterized human umbilical vein endothelial cell HUVEC adhesion, proliferation, and gene expression on bilayered polyelectrolyte coatings composed of an outermost layer of glycosaminoglycans hyaluronan, heparin, or chondroitin sulfate, with an underlying layer of poly-l-lysine or chitosan. The proportion of cells that adhered to the various polyelectrolyte coatings after 1 and 2 h incubations was quantified by the WST-8 assay. Interchanging poly-l-lysine with chitosan resulted in significant differences in cellular adhesion to the outermost glycosaminoglycan layer after 1 h, but these differences became insignificant after 2 h. The proliferation of HUVEC on the various bilayered polyelectrolyte coatings over 10 days was characterized using the WST-8 assay. Regardless of whether the underlying layer was poly-l-lysine or chitosan, HUVEC proliferation on the hyaluronan outermost layer was significantly less than on heparin or chondroitin sulfate. Additionally, it was observed that there was more proliferation with poly-l-lysine as the underlying layer, compared to chitosan. Subsequently, real-time polymerase chain reaction was used to analyze the expression of seven genes related to adhesion, migration, and endothelial function (VWF, VEGFR, VEGFA, endoglin, integrin-α5, ICAM1, and ICAM2 by HUVEC cultured on the various bilayered polyelectrolyte coatings for 3 days. With poly-l-lysine as the underlying layer, biologically significant differences greater than twofold in the expression of VWF, VEGFR, VEGFA, endoglin, and ICAM1 were observed among the three glycosaminoglycans. With chitosan as the underlying layer, all three glycosaminoglycans displayed biologically significant differences in the expression of VWF and VEGFR compared to the chitosan control. CT-HA displayed the highest level of expression of VWF, whereas expression levels of VEGFR were almost similar among the three glycosaminoglycans. Published version 2012-10-05T03:11:55Z 2019-12-06T13:37:55Z 2012-10-05T03:11:55Z 2019-12-06T13:37:55Z 2010 2010 Journal Article Heng, B. C., Bezerra, P. P., Meng, Q. R., Chin, D. W. L., Koh, L. B., Li, H., et al. (2010). Adhesion, proliferation, and gene expression profile of human umbilical vein endothelial cells cultured on bilayered polyelectrolyte coatings composed of glycosaminoglycans. Biointerphases, 5(3). 1934-8630 https://hdl.handle.net/10356/79974 http://hdl.handle.net/10220/8709 10.1116/1.3483218 en Biointerphases © 2010 American Vacuum Society This paper was published in Biointerphases and is made available as an electronic reprint (preprint) with permission of American Vacuum Society. The paper can be found at the following official URL: [http://dx.doi.org/10.1116/1.3483218 ].  One print or electronic copy may be made for personal use only. Systematic or multiple reproduction, distribution to multiple locations via electronic or other means, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper is prohibited and is subject to penalties under law. application/pdf
institution Nanyang Technological University
building NTU Library
continent Asia
country Singapore
Singapore
content_provider NTU Library
collection DR-NTU
language English
topic DRNTU::Science::Medicine::Biomedical engineering
spellingShingle DRNTU::Science::Medicine::Biomedical engineering
Boey, Freddy Yin Chiang
Heng, Boon Chin
Bezerra, Paula Porto
Meng, Qing Rui
Chin, Desmond Wai-Loon
Koh, Li Buay
Li, Hai
Zhang, Hua
Preiser, Peter Rainer
Venkatraman, Subbu S.
Adhesion, proliferation, and gene expression profile of human umbilical vein endothelial cells cultured on bilayered polyelectrolyte coatings composed of glycosaminoglycans
description This study characterized human umbilical vein endothelial cell HUVEC adhesion, proliferation, and gene expression on bilayered polyelectrolyte coatings composed of an outermost layer of glycosaminoglycans hyaluronan, heparin, or chondroitin sulfate, with an underlying layer of poly-l-lysine or chitosan. The proportion of cells that adhered to the various polyelectrolyte coatings after 1 and 2 h incubations was quantified by the WST-8 assay. Interchanging poly-l-lysine with chitosan resulted in significant differences in cellular adhesion to the outermost glycosaminoglycan layer after 1 h, but these differences became insignificant after 2 h. The proliferation of HUVEC on the various bilayered polyelectrolyte coatings over 10 days was characterized using the WST-8 assay. Regardless of whether the underlying layer was poly-l-lysine or chitosan, HUVEC proliferation on the hyaluronan outermost layer was significantly less than on heparin or chondroitin sulfate. Additionally, it was observed that there was more proliferation with poly-l-lysine as the underlying layer, compared to chitosan. Subsequently, real-time polymerase chain reaction was used to analyze the expression of seven genes related to adhesion, migration, and endothelial function (VWF, VEGFR, VEGFA, endoglin, integrin-α5, ICAM1, and ICAM2 by HUVEC cultured on the various bilayered polyelectrolyte coatings for 3 days. With poly-l-lysine as the underlying layer, biologically significant differences greater than twofold in the expression of VWF, VEGFR, VEGFA, endoglin, and ICAM1 were observed among the three glycosaminoglycans. With chitosan as the underlying layer, all three glycosaminoglycans displayed biologically significant differences in the expression of VWF and VEGFR compared to the chitosan control. CT-HA displayed the highest level of expression of VWF, whereas expression levels of VEGFR were almost similar among the three glycosaminoglycans.
author2 School of Materials Science & Engineering
author_facet School of Materials Science & Engineering
Boey, Freddy Yin Chiang
Heng, Boon Chin
Bezerra, Paula Porto
Meng, Qing Rui
Chin, Desmond Wai-Loon
Koh, Li Buay
Li, Hai
Zhang, Hua
Preiser, Peter Rainer
Venkatraman, Subbu S.
format Article
author Boey, Freddy Yin Chiang
Heng, Boon Chin
Bezerra, Paula Porto
Meng, Qing Rui
Chin, Desmond Wai-Loon
Koh, Li Buay
Li, Hai
Zhang, Hua
Preiser, Peter Rainer
Venkatraman, Subbu S.
author_sort Boey, Freddy Yin Chiang
title Adhesion, proliferation, and gene expression profile of human umbilical vein endothelial cells cultured on bilayered polyelectrolyte coatings composed of glycosaminoglycans
title_short Adhesion, proliferation, and gene expression profile of human umbilical vein endothelial cells cultured on bilayered polyelectrolyte coatings composed of glycosaminoglycans
title_full Adhesion, proliferation, and gene expression profile of human umbilical vein endothelial cells cultured on bilayered polyelectrolyte coatings composed of glycosaminoglycans
title_fullStr Adhesion, proliferation, and gene expression profile of human umbilical vein endothelial cells cultured on bilayered polyelectrolyte coatings composed of glycosaminoglycans
title_full_unstemmed Adhesion, proliferation, and gene expression profile of human umbilical vein endothelial cells cultured on bilayered polyelectrolyte coatings composed of glycosaminoglycans
title_sort adhesion, proliferation, and gene expression profile of human umbilical vein endothelial cells cultured on bilayered polyelectrolyte coatings composed of glycosaminoglycans
publishDate 2012
url https://hdl.handle.net/10356/79974
http://hdl.handle.net/10220/8709
_version_ 1759853113543491584