Immunofluorescence protects RNA signals in simultaneous RNA–DNA FISH

• Main Authors: Lai, Lan-Tian, Lee, Pin Jie, Zhang, Li-Feng
• Other Authors: School of Biological Sciences
• Format: Article
• Language: English
• Published: 2015
• Subjects: Simultaneous RNA–DNA FISH; X chromosome inactivation (XCI); Terra; Fluorescence in situ hybridization (FISH); Xist
• Online Access: https://hdl.handle.net/10356/80848; http://hdl.handle.net/10220/38893
• Institution: Nanyang Technological University

Cell research often requires combinational detection of RNA and DNA by fluorescence in situ hybridization (RNA–DNA FISH). However, it is difficult to preserve the fragile RNA signals through the harsh conditions used to denature the DNA template in DNA FISH. The current protocols of RNA –DNA FISH st...