An excitatory GABA loop operating in vivo

While it has been proposed that the conventional inhibitory neurotransmitter GABA can be excitatory in the mammalian brain, much remains to be learned concerning the circumstances and the cellular mechanisms governing potential excitatory GABA action. Using a combination of optogenetics and two-phot...

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Main Authors: Astorga, Guadalupe, Bao, Jin, Marty, Alain, Augustine, George James, Franconville, Romain, Jalil, Abdelali, Bradley, Jonathan, Llano, Isabel
Other Authors: Lee Kong Chian School of Medicine (LKCMedicine)
Format: Article
Language:English
Published: 2015
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Online Access:https://hdl.handle.net/10356/81000
http://hdl.handle.net/10220/39040
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Institution: Nanyang Technological University
Language: English
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spelling sg-ntu-dr.10356-810002022-02-16T16:31:22Z An excitatory GABA loop operating in vivo Astorga, Guadalupe Bao, Jin Marty, Alain Augustine, George James Franconville, Romain Jalil, Abdelali Bradley, Jonathan Llano, Isabel Lee Kong Chian School of Medicine (LKCMedicine) GABA Interneurons Calcium Cerebellum Parallel fibers While it has been proposed that the conventional inhibitory neurotransmitter GABA can be excitatory in the mammalian brain, much remains to be learned concerning the circumstances and the cellular mechanisms governing potential excitatory GABA action. Using a combination of optogenetics and two-photon calcium imaging in vivo, we find that activation of chloride-permeable GABAA receptors in parallel fibers (PFs) of the cerebellar molecular layer of adult mice causes parallel fiber excitation. Stimulation of PFs at submaximal stimulus intensities leads to GABA release from molecular layer interneurons (MLIs), thus creating a positive feedback loop that enhances excitation near the center of an activated PF bundle. Our results imply that elevated chloride concentration can occur in specific intracellular compartments of mature mammalian neurons and suggest an excitatory role for GABAA receptors in the cerebellar cortex of adult mice. Published version 2015-12-10T08:16:23Z 2019-12-06T14:19:16Z 2015-12-10T08:16:23Z 2019-12-06T14:19:16Z 2015 Journal Article Astorga, G., Bao, J., Marty, A., Augustine, G. J., Franconville, R., Jalil, A., et al. (2015). An excitatory GABA loop operating in vivo. Frontiers in Cellular Neuroscience, 9, 275-. 1662-5102 https://hdl.handle.net/10356/81000 http://hdl.handle.net/10220/39040 10.3389/fncel.2015.00275 26236197 en Frontiers in Cellular Neuroscience © 2015 Astorga, Bao, Marty, Augustine, Franconville, Jalil, Bradley and Llano. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution and reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. 14 p. application/pdf
institution Nanyang Technological University
building NTU Library
continent Asia
country Singapore
Singapore
content_provider NTU Library
collection DR-NTU
language English
topic GABA
Interneurons
Calcium
Cerebellum
Parallel fibers
spellingShingle GABA
Interneurons
Calcium
Cerebellum
Parallel fibers
Astorga, Guadalupe
Bao, Jin
Marty, Alain
Augustine, George James
Franconville, Romain
Jalil, Abdelali
Bradley, Jonathan
Llano, Isabel
An excitatory GABA loop operating in vivo
description While it has been proposed that the conventional inhibitory neurotransmitter GABA can be excitatory in the mammalian brain, much remains to be learned concerning the circumstances and the cellular mechanisms governing potential excitatory GABA action. Using a combination of optogenetics and two-photon calcium imaging in vivo, we find that activation of chloride-permeable GABAA receptors in parallel fibers (PFs) of the cerebellar molecular layer of adult mice causes parallel fiber excitation. Stimulation of PFs at submaximal stimulus intensities leads to GABA release from molecular layer interneurons (MLIs), thus creating a positive feedback loop that enhances excitation near the center of an activated PF bundle. Our results imply that elevated chloride concentration can occur in specific intracellular compartments of mature mammalian neurons and suggest an excitatory role for GABAA receptors in the cerebellar cortex of adult mice.
author2 Lee Kong Chian School of Medicine (LKCMedicine)
author_facet Lee Kong Chian School of Medicine (LKCMedicine)
Astorga, Guadalupe
Bao, Jin
Marty, Alain
Augustine, George James
Franconville, Romain
Jalil, Abdelali
Bradley, Jonathan
Llano, Isabel
format Article
author Astorga, Guadalupe
Bao, Jin
Marty, Alain
Augustine, George James
Franconville, Romain
Jalil, Abdelali
Bradley, Jonathan
Llano, Isabel
author_sort Astorga, Guadalupe
title An excitatory GABA loop operating in vivo
title_short An excitatory GABA loop operating in vivo
title_full An excitatory GABA loop operating in vivo
title_fullStr An excitatory GABA loop operating in vivo
title_full_unstemmed An excitatory GABA loop operating in vivo
title_sort excitatory gaba loop operating in vivo
publishDate 2015
url https://hdl.handle.net/10356/81000
http://hdl.handle.net/10220/39040
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