Determination of the archaeal and bacterial communities in two-phase and single-stage anaerobic systems by 454 pyrosequencing
2-Phase anaerobic digestion (AD), where the acidogenic phase was operated at 2 day hydraulic retention time (HRT) and the methanogenic phase at 10 days HRT, had been evaluated to determine if it could provide higher organic reduction and methane production than the conventional single-stage AD (also...
Saved in:
Main Authors: | , , , , |
---|---|
Other Authors: | |
Format: | Article |
Language: | English |
Published: |
2015
|
Subjects: | |
Online Access: | https://hdl.handle.net/10356/81113 http://hdl.handle.net/10220/39087 |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
Institution: | Nanyang Technological University |
Language: | English |
id |
sg-ntu-dr.10356-81113 |
---|---|
record_format |
dspace |
spelling |
sg-ntu-dr.10356-811132020-09-26T22:02:59Z Determination of the archaeal and bacterial communities in two-phase and single-stage anaerobic systems by 454 pyrosequencing Maspolim, Yogananda Zhou, Yan Guo, Chenghong Xiao, Keke Ng, Wun Jern School of Civil and Environmental Engineering Nanyang Environment and Water Research Institute Methanogen Sewage sludge 2-Phase anaerobic digestion Microbial community 454 pyrosequencing 2-Phase anaerobic digestion (AD), where the acidogenic phase was operated at 2 day hydraulic retention time (HRT) and the methanogenic phase at 10 days HRT, had been evaluated to determine if it could provide higher organic reduction and methane production than the conventional single-stage AD (also operated at 12 days HRT). 454 pyrosequencing was performed to determine and compare the microbial communities. The acidogenic reactor of the 2-phase system yielded a unique bacterial community of the lowest richness and diversity, while bacterial profiles of the methanogenic reactor closely followed the single-stage reactor. All reactors were predominated by hydrogenotrophic methanogens, mainly Methanolinea. Unusually, the acidogenic reactor contributed up to 24% of total methane production in the 2-phase system. This could be explained by the presence of Methanosarcina and Methanobrevibacter, and their activities could also help regulate reactor alkalinity during high loading conditions through carbon dioxide production. The enrichment of hydrolytic and acidogenic Porphyromonadaceae, Prevotellaceae, Ruminococcaceae and unclassified Bacteroidetes in the acidogenic reactor would have contributed to the improved sludge volatile solids degradation, and ultimately the overall 2-phase system's performance. Syntrophic acetogenic microorganisms were absent in the acidogenic reactor but present in the downstream methanogenic reactor, indicating the retention of various metabolic pathways also found in a single-stage system. The determination of key microorganisms further expands our understanding of the complex biological functions in AD process. NRF (Natl Research Foundation, S’pore) Published version 2015-12-15T02:11:16Z 2019-12-06T14:21:40Z 2015-12-15T02:11:16Z 2019-12-06T14:21:40Z 2015 Journal Article Maspolim, Y., Zhou, Y., Guo, C., Xiao, K., & Ng, W. J. (2015). Determination of the archaeal and bacterial communities in two-phase and single-stage anaerobic systems by 454 pyrosequencing. Journal of Environmental Sciences, 36, 121-129. 1001-0742 https://hdl.handle.net/10356/81113 http://hdl.handle.net/10220/39087 10.1016/j.jes.2015.02.017 en Journal of Environmental Sciences © 2015 The Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences. This paper was published in Journal of Environmental Sciences and is made available as an electronic reprint (preprint) with permission of The Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences. The published version is available at: [http://dx.doi.org/10.1016/j.jes.2015.02.017]. One print or electronic copy may be made for personal use only. Systematic or multiple reproduction, distribution to multiple locations via electronic or other means, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper is prohibited and is subject to penalties under law. 9 p. application/pdf |
institution |
Nanyang Technological University |
building |
NTU Library |
country |
Singapore |
collection |
DR-NTU |
language |
English |
topic |
Methanogen Sewage sludge 2-Phase anaerobic digestion Microbial community 454 pyrosequencing |
spellingShingle |
Methanogen Sewage sludge 2-Phase anaerobic digestion Microbial community 454 pyrosequencing Maspolim, Yogananda Zhou, Yan Guo, Chenghong Xiao, Keke Ng, Wun Jern Determination of the archaeal and bacterial communities in two-phase and single-stage anaerobic systems by 454 pyrosequencing |
description |
2-Phase anaerobic digestion (AD), where the acidogenic phase was operated at 2 day hydraulic retention time (HRT) and the methanogenic phase at 10 days HRT, had been evaluated to determine if it could provide higher organic reduction and methane production than the conventional single-stage AD (also operated at 12 days HRT). 454 pyrosequencing was performed to determine and compare the microbial communities. The acidogenic reactor of the 2-phase system yielded a unique bacterial community of the lowest richness and diversity, while bacterial profiles of the methanogenic reactor closely followed the single-stage reactor. All reactors were predominated by hydrogenotrophic methanogens, mainly Methanolinea. Unusually, the acidogenic reactor contributed up to 24% of total methane production in the 2-phase system. This could be explained by the presence of Methanosarcina and Methanobrevibacter, and their activities could also help regulate reactor alkalinity during high loading conditions through carbon dioxide production. The enrichment of hydrolytic and acidogenic Porphyromonadaceae, Prevotellaceae, Ruminococcaceae and unclassified Bacteroidetes in the acidogenic reactor would have contributed to the improved sludge volatile solids degradation, and ultimately the overall 2-phase system's performance. Syntrophic acetogenic microorganisms were absent in the acidogenic reactor but present in the downstream methanogenic reactor, indicating the retention of various metabolic pathways also found in a single-stage system. The determination of key microorganisms further expands our understanding of the complex biological functions in AD process. |
author2 |
School of Civil and Environmental Engineering |
author_facet |
School of Civil and Environmental Engineering Maspolim, Yogananda Zhou, Yan Guo, Chenghong Xiao, Keke Ng, Wun Jern |
format |
Article |
author |
Maspolim, Yogananda Zhou, Yan Guo, Chenghong Xiao, Keke Ng, Wun Jern |
author_sort |
Maspolim, Yogananda |
title |
Determination of the archaeal and bacterial communities in two-phase and single-stage anaerobic systems by 454 pyrosequencing |
title_short |
Determination of the archaeal and bacterial communities in two-phase and single-stage anaerobic systems by 454 pyrosequencing |
title_full |
Determination of the archaeal and bacterial communities in two-phase and single-stage anaerobic systems by 454 pyrosequencing |
title_fullStr |
Determination of the archaeal and bacterial communities in two-phase and single-stage anaerobic systems by 454 pyrosequencing |
title_full_unstemmed |
Determination of the archaeal and bacterial communities in two-phase and single-stage anaerobic systems by 454 pyrosequencing |
title_sort |
determination of the archaeal and bacterial communities in two-phase and single-stage anaerobic systems by 454 pyrosequencing |
publishDate |
2015 |
url |
https://hdl.handle.net/10356/81113 http://hdl.handle.net/10220/39087 |
_version_ |
1681059373912162304 |