A Rapid and Quantitative Fluorimetric Method for Protein-Targeting Small Molecule Drug Screening

• Main Authors: Yu, Yong, New, Siu Yee, Lin, Jiaxian, Su, Xiaodi, Tan, Yen Nee
• Other Authors: School of Chemical and Biomedical Engineering
• Format: Article
• Language: English
• Published: 2015
• Subjects: Fluorescence; Binding constant; Stability; Issue 104; Gold nanoclusters; Synthesis; Human serum albumin; Drug screening; Protein unfolding; Bioengineering
• Online Access: https://hdl.handle.net/10356/81171; http://hdl.handle.net/10220/39185
• Institution: Nanyang Technological University

We demonstrate a new drug screening method for determining the binding affinity of small drug molecules to a target protein by forming fluorescent gold nanoclusters (Au NCs) within the drug-loaded protein, based on the differential fluorescence signal emitted by the Au NCs. Albumin proteins such as...