Sedimentation Equilibrium of a Small Oligomer-forming Membrane Protein: Effect of Histidine Protonation on Pentameric Stability

• Main Authors: Surya, Wahyu, Torres, Jaume
• 其他作者: School of Biological Sciences
• 格式: Article
• 語言: English
• 出版: 2015
• 主題: Chemistry; Issue 98; Analytical ultracentrifugation; sedimentation equilibrium; molecular weight; membrane proteins; small hydrophobic; respiratory syncytial virus; detergent; density matching; oligomeric size; histidine protonation; oligomer stability; Issue 98; Sedimentation equilibrium; Analytical ultracentrifugation; Molecular weight; Membrane proteins; Small hydrophobic
• 在線閱讀: https://hdl.handle.net/10356/81230; http://hdl.handle.net/10220/39183

Analytical ultracentrifugation (AUC) can be used to study reversible interactions between macromolecules over a wide range of interaction strengths and under physiological conditions. This makes AUC a method of choice to quantitatively assess stoichiometry and thermodynamics of homo- and hetero-asso...