Fluorospectroscopy of Dye-Loaded Liposomes in Photonic Crystal Fibers
The immobilization and probing of liposomes within photonic crystal fibers was demonstrated for the first time. A bioactive surface was used to tether the liposomes. This bioactive surface consisted of streptavidin bound to a photochemically functionalized biotin layer. Bound streptavidin, hence, en...
Saved in:
| Main Authors: | , , , |
|---|---|
| 其他作者: | |
| 格式: | Article |
| 語言: | English |
| 出版: |
2016
|
| 主題: | |
| 在線閱讀: | https://hdl.handle.net/10356/81405 http://hdl.handle.net/10220/40743 |
| 標簽: |
添加標簽
沒有標簽, 成為第一個標記此記錄!
|
| 機構: | Nanyang Technological University |
| 語言: | English |
| 總結: | The immobilization and probing of liposomes within photonic crystal fibers was demonstrated for the first time. A bioactive surface was used to tether the liposomes. This bioactive surface consisted of streptavidin bound to a photochemically functionalized biotin layer. Bound streptavidin, hence, enabled the further binding of biotinylated dye-loaded liposomes. In-fiber fluorescence spectroscopy was used to quantify the streptavidin coverage density. The same method was also used to characterize the surface-tethered liposomes. The further observation of a unique phenomenon-photobleaching dequenching-was used for the first time as an indication of liposomal content retention. This indicated no rupturing of liposomes, highlighting them as bioderived analogues to dye-doped nanoparticles. The demonstrated integration of liposomes with optical waveguides shows potential as a biointegrated photonic device. |
|---|