Cleavage efficient 2A peptides for high level monoclonal antibody expression in CHO cells

• Main Authors: Chng, Jake, Wang, Tianhua, Nian, Rui, Lau, Ally, Hoi, Kong Meng, Yang, Yuansheng, Ho, Steven C. L., Gagnon, Peter, Bi, Xuezhi
• Other Authors: School of Chemical and Biomedical Engineering
• Format: Article
• Language: English
• Published: 2016
• Subjects: monoclonal antibody; 2A peptide; furin; CHO; cleavage efficiency; GSG linker
• Online Access: https://hdl.handle.net/10356/81450; http://hdl.handle.net/10220/40785
• Institution: Nanyang Technological University

Linking the heavy chain (HC) and light chain (LC) genes required for monoclonal antibodies (mAb) production on a single cassette using 2A peptides allows control of LC and HC ratio and reduces non-expressing cells. Four 2A peptides derived from the foot-and-mouth disease virus (F2A), equine rhinitis...