“Protein” Measurement in Biological Wastewater Treatment Systems: A Critical Evaluation

Five commercially available assay kits were tested on the same protein sample with the addition of 17 different types of interfering substances typically found in the biological wastewater treatment, and a comparison of the use of these assays with 22 different protein and peptide samples is also pr...

Full description

Saved in:
Bibliographic Details
Main Authors: Le, Chencheng, Kunacheva, Chinagarn, Stuckey, David C.
Other Authors: School of Civil and Environmental Engineering
Format: Article
Language:English
Published: 2016
Subjects:
Online Access:https://hdl.handle.net/10356/81453
http://hdl.handle.net/10220/40796
Tags: Add Tag
No Tags, Be the first to tag this record!
Institution: Nanyang Technological University
Language: English
id sg-ntu-dr.10356-81453
record_format dspace
spelling sg-ntu-dr.10356-814532020-09-26T22:01:36Z “Protein” Measurement in Biological Wastewater Treatment Systems: A Critical Evaluation Le, Chencheng Kunacheva, Chinagarn Stuckey, David C. School of Civil and Environmental Engineering Nanyang Environment and Water Research Institute Civil and Environmental Engineering Five commercially available assay kits were tested on the same protein sample with the addition of 17 different types of interfering substances typically found in the biological wastewater treatment, and a comparison of the use of these assays with 22 different protein and peptide samples is also presented. It was shown that a wide variety of substances can interfere dramatically with these assays; the metachromatic response was also clearly influenced by different proteinaceous material. Measurement of the "protein" content in the effluent of an anaerobic membrane bioreactor was then carried out using these assay methods. Quantitative results of the "protein" concentration in the different effluent samples, with or without spiked additions of Bovine Serum Albumin (BSA), showed considerable disagreement. We concluded that the "protein" measured in wastewater samples using standard colorimetric assays often shows false positive results and has little correlation to their real value. A new analytical method needs to be developed in order to gain greater insight into the biological transformations occurring in anaerobic digestion, and how soluble microbial products (SMPs) are produced. NRF (Natl Research Foundation, S’pore) Accepted version 2016-06-27T04:26:30Z 2019-12-06T14:31:20Z 2016-06-27T04:26:30Z 2019-12-06T14:31:20Z 2016 Journal Article Le, C., Kunacheva, C., & Stuckey, D. C. (2016). “Protein” Measurement in Biological Wastewater Treatment Systems: A Critical Evaluation. Environmental Science & Technology, 50(6), 3074-3081. 0013-936X https://hdl.handle.net/10356/81453 http://hdl.handle.net/10220/40796 10.1021/acs.est.5b05261 en Environmental Science & Technology © 2016 American Chemical Society. This is the author created version of a work that has been peer reviewed and accepted for publication by Environmental Science & Technology, American Chemical Society. It incorporates referee’s comments but changes resulting from the publishing process, such as copyediting, structural formatting, may not be reflected in this document. The published version is available at: [http://dx.doi.org/10.1021/acs.est.5b05261]. 8 p. application/pdf
institution Nanyang Technological University
building NTU Library
country Singapore
collection DR-NTU
language English
topic Civil and Environmental Engineering
spellingShingle Civil and Environmental Engineering
Le, Chencheng
Kunacheva, Chinagarn
Stuckey, David C.
“Protein” Measurement in Biological Wastewater Treatment Systems: A Critical Evaluation
description Five commercially available assay kits were tested on the same protein sample with the addition of 17 different types of interfering substances typically found in the biological wastewater treatment, and a comparison of the use of these assays with 22 different protein and peptide samples is also presented. It was shown that a wide variety of substances can interfere dramatically with these assays; the metachromatic response was also clearly influenced by different proteinaceous material. Measurement of the "protein" content in the effluent of an anaerobic membrane bioreactor was then carried out using these assay methods. Quantitative results of the "protein" concentration in the different effluent samples, with or without spiked additions of Bovine Serum Albumin (BSA), showed considerable disagreement. We concluded that the "protein" measured in wastewater samples using standard colorimetric assays often shows false positive results and has little correlation to their real value. A new analytical method needs to be developed in order to gain greater insight into the biological transformations occurring in anaerobic digestion, and how soluble microbial products (SMPs) are produced.
author2 School of Civil and Environmental Engineering
author_facet School of Civil and Environmental Engineering
Le, Chencheng
Kunacheva, Chinagarn
Stuckey, David C.
format Article
author Le, Chencheng
Kunacheva, Chinagarn
Stuckey, David C.
author_sort Le, Chencheng
title “Protein” Measurement in Biological Wastewater Treatment Systems: A Critical Evaluation
title_short “Protein” Measurement in Biological Wastewater Treatment Systems: A Critical Evaluation
title_full “Protein” Measurement in Biological Wastewater Treatment Systems: A Critical Evaluation
title_fullStr “Protein” Measurement in Biological Wastewater Treatment Systems: A Critical Evaluation
title_full_unstemmed “Protein” Measurement in Biological Wastewater Treatment Systems: A Critical Evaluation
title_sort “protein” measurement in biological wastewater treatment systems: a critical evaluation
publishDate 2016
url https://hdl.handle.net/10356/81453
http://hdl.handle.net/10220/40796
_version_ 1681058730705158144