IgG Aggregation Mechanism for CHO Cell Lines Expressing Excess Heavy Chains

• Main Authors: Ho, Steven C. L., Wang, Tianhua, Song, Zhiwei, Yang, Yuansheng
• Other Authors: School of Chemical and Biomedical Engineering
• Format: Article
• Language: English
• Published: 2016
• Subjects: Monoclonal antibody; Chinese hamster ovary cells; BiP; IgG aggregation
• Online Access: https://hdl.handle.net/10356/81469; http://hdl.handle.net/10220/40791
• Institution: Nanyang Technological University

Aggregates in protein therapeutics like IgG monoclonal antibodies (mAb) are detrimental to product safety and efficacy. It has been reported that aggregates form in Chinese hamster ovary (CHO) cell lines expressing greater amount of heavy chain (HC) than light chain (LC). In this study, we observed...