Membrane feeding of dengue patient’s blood as a substitute for direct skin feeding in studying Aedes-dengue virus interaction

Membrane feeding of dengue patient’s blood as a substitute for direct skin feeding in studying Aedes-dengue virus interaction

Background: Understanding the interaction between Aedes vectors and dengue viruses (DENV) has significant implications in determining the transmission dynamics of dengue. The absence of an animal model and ethical concerns regarding direct feeding of mosquitoes on patients has resulted in most infec...

Full description

Saved in:
Bibliographic Details
Main Authors: Tan, Cheong-Huat, Wong, Pei-Sze Jeslyn, Li, Mei-Zhi Irene, Yang, Hui-Ting, Chong, Chee-Seng, Lee, Linda K., Yuan, Shi, Leo, Yee-Sin, Ng, Lee-Ching, Lye, David C.
Other Authors: School of Biological Sciences
Format: Article
Language:English
Published: 2016
Subjects:
Dengue
Membrane Feeding Assay (MFA)
EDTA blood
Aedes aegypti
Direct Skin Feeding Assay (DFSA)
Online Access:https://hdl.handle.net/10356/81506
http://hdl.handle.net/10220/40807
Tags: Add Tag
No Tags, Be the first to tag this record!
Institution: Nanyang Technological University
Language: English
Description
Summary:Background: Understanding the interaction between Aedes vectors and dengue viruses (DENV) has significant implications in determining the transmission dynamics of dengue. The absence of an animal model and ethical concerns regarding direct feeding of mosquitoes on patients has resulted in most infection studies using blood meals spiked with laboratory-cultured DENV. Data obtained from such studies may not reflect the natural human-mosquito transmission scenario. This study explored the potential of using membrane feeding of dengue patient’s blood as a substitute for direct skin feeding.

Similar Items