Extraction of plant-based capsules for microencapsulation applications

Microcapsules derived from plant-based spores or pollen provide a robust platform for a diverse range of microencapsulation applications. Sporopollenin exine capsules (SECs) are obtained when spores or pollen are processed so as to remove the internal sporoplasmic contents. The resulting hollow micr...

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Main Authors: Potroz, Michael G., Mundargi, Raghavendra C., Park, Jae Hyeon, Tan, Ee-Lin, Cho, Nam-joon
Other Authors: School of Chemical and Biomedical Engineering
Format: Article
Language:English
Published: 2019
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Online Access:https://hdl.handle.net/10356/81541
http://hdl.handle.net/10220/47495
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Institution: Nanyang Technological University
Language: English
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spelling sg-ntu-dr.10356-815412023-07-14T15:53:19Z Extraction of plant-based capsules for microencapsulation applications Potroz, Michael G. Mundargi, Raghavendra C. Park, Jae Hyeon Tan, Ee-Lin Cho, Nam-joon School of Chemical and Biomedical Engineering School of Materials Science & Engineering Centre for Biomimetic Sensor Science Bioengineering Lycopodium clavatum DRNTU::Engineering::Bioengineering Microcapsules derived from plant-based spores or pollen provide a robust platform for a diverse range of microencapsulation applications. Sporopollenin exine capsules (SECs) are obtained when spores or pollen are processed so as to remove the internal sporoplasmic contents. The resulting hollow microcapsules exhibit a high degree of micromeritic uniformity and retain intricate microstructural features related to the particular plant species. Herein, we demonstrate a streamlined process for the production of SECs from Lycopodium clavatum spores and for the loading of hydrophilic compounds into these SECs. The current SEC isolation procedure has been recently optimized to significantly reduce the processing requirements which are conventionally used in SEC isolation, and to ensure the production of intact microcapsules. Natural L. clavatum spores are defatted with acetone, treated with phosphoric acid, and extensively washed to remove sporoplasmic contents. After acetone defatting, a single processing step using 85% phosphoric acid has been shown to remove all sporoplasmic contents. By limiting the acid processing time to 30 hr, it is possible to isolate clean SECs and avoid SEC fracturing, which has been shown to occur with prolonged processing time. Extensive washing with water, dilute acids, dilute bases, and solvents ensures that all sporoplasmic material and chemical residues are adequately removed. The vacuum loading technique is utilized to load a model protein (Bovine Serum Albumin) as a representative hydrophilic compound. Vacuum loading provides a simple technique to load various compounds without the need for harsh solvents or undesirable chemicals which are often required in other microencapsulation protocols. Based on these isolation and loading protocols, SECs provide a promising material for use in a diverse range of microencapsulation applications, such as, therapeutics, foods, cosmetics, and personal care products. NRF (Natl Research Foundation, S’pore) NMRC (Natl Medical Research Council, S’pore) Published version 2019-01-16T08:14:02Z 2019-12-06T14:33:18Z 2019-01-16T08:14:02Z 2019-12-06T14:33:18Z 2016 Journal Article Potroz, M. G., Mundargi, R. C., Park, J. H., Tan, E.-L., & Cho, N.-j. (2016). Extraction of plant-based capsules for microencapsulation applications. Journal of Visualized Experiments, (117), e54768-. doi:10.3791/54768 1940-087X https://hdl.handle.net/10356/81541 http://hdl.handle.net/10220/47495 10.3791/54768 en Journal of Visualized Experiments © 2016 Journal of Visualized Experiments. All rights reserved. This paper was published in Journal of Visualized Experiments and is made available with permission of Journal of Visualized Experiments. 10 p. application/pdf
institution Nanyang Technological University
building NTU Library
continent Asia
country Singapore
Singapore
content_provider NTU Library
collection DR-NTU
language English
topic Bioengineering
Lycopodium clavatum
DRNTU::Engineering::Bioengineering
spellingShingle Bioengineering
Lycopodium clavatum
DRNTU::Engineering::Bioengineering
Potroz, Michael G.
Mundargi, Raghavendra C.
Park, Jae Hyeon
Tan, Ee-Lin
Cho, Nam-joon
Extraction of plant-based capsules for microencapsulation applications
description Microcapsules derived from plant-based spores or pollen provide a robust platform for a diverse range of microencapsulation applications. Sporopollenin exine capsules (SECs) are obtained when spores or pollen are processed so as to remove the internal sporoplasmic contents. The resulting hollow microcapsules exhibit a high degree of micromeritic uniformity and retain intricate microstructural features related to the particular plant species. Herein, we demonstrate a streamlined process for the production of SECs from Lycopodium clavatum spores and for the loading of hydrophilic compounds into these SECs. The current SEC isolation procedure has been recently optimized to significantly reduce the processing requirements which are conventionally used in SEC isolation, and to ensure the production of intact microcapsules. Natural L. clavatum spores are defatted with acetone, treated with phosphoric acid, and extensively washed to remove sporoplasmic contents. After acetone defatting, a single processing step using 85% phosphoric acid has been shown to remove all sporoplasmic contents. By limiting the acid processing time to 30 hr, it is possible to isolate clean SECs and avoid SEC fracturing, which has been shown to occur with prolonged processing time. Extensive washing with water, dilute acids, dilute bases, and solvents ensures that all sporoplasmic material and chemical residues are adequately removed. The vacuum loading technique is utilized to load a model protein (Bovine Serum Albumin) as a representative hydrophilic compound. Vacuum loading provides a simple technique to load various compounds without the need for harsh solvents or undesirable chemicals which are often required in other microencapsulation protocols. Based on these isolation and loading protocols, SECs provide a promising material for use in a diverse range of microencapsulation applications, such as, therapeutics, foods, cosmetics, and personal care products.
author2 School of Chemical and Biomedical Engineering
author_facet School of Chemical and Biomedical Engineering
Potroz, Michael G.
Mundargi, Raghavendra C.
Park, Jae Hyeon
Tan, Ee-Lin
Cho, Nam-joon
format Article
author Potroz, Michael G.
Mundargi, Raghavendra C.
Park, Jae Hyeon
Tan, Ee-Lin
Cho, Nam-joon
author_sort Potroz, Michael G.
title Extraction of plant-based capsules for microencapsulation applications
title_short Extraction of plant-based capsules for microencapsulation applications
title_full Extraction of plant-based capsules for microencapsulation applications
title_fullStr Extraction of plant-based capsules for microencapsulation applications
title_full_unstemmed Extraction of plant-based capsules for microencapsulation applications
title_sort extraction of plant-based capsules for microencapsulation applications
publishDate 2019
url https://hdl.handle.net/10356/81541
http://hdl.handle.net/10220/47495
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