Impedimetric cell-based biosensor for real-time monitoring of cytopathic effects induced by dengue viruses

We describe an impedimetric cell-based biosensor constructed from poly-l-lysine (PLL)-modified screen-printed carbon electrode for real-time monitoring of dengue virus (DENV) infection of surface-immobilized baby hamster kidney (BHK-21) fibroblast cells. Cytopathic effects (CPE) induced by DENV-2 Ne...

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Main Authors: Cheng, Ming Soon, Lau, Suk Hiang, Chan, Kwai Peng, Toh, Chee-Seng, Chow, Vincent T.
Other Authors: School of Physical and Mathematical Sciences
Format: Article
Language:English
Published: 2016
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Online Access:https://hdl.handle.net/10356/81633
http://hdl.handle.net/10220/40877
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Institution: Nanyang Technological University
Language: English
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spelling sg-ntu-dr.10356-816332020-03-07T12:31:29Z Impedimetric cell-based biosensor for real-time monitoring of cytopathic effects induced by dengue viruses Cheng, Ming Soon Lau, Suk Hiang Chan, Kwai Peng Toh, Chee-Seng Chow, Vincent T. School of Physical and Mathematical Sciences Cytopathic effects Dengue virus serotypes We describe an impedimetric cell-based biosensor constructed from poly-l-lysine (PLL)-modified screen-printed carbon electrode for real-time monitoring of dengue virus (DENV) infection of surface-immobilized baby hamster kidney (BHK-21) fibroblast cells. Cytopathic effects (CPE) induced by DENV-2 New Guinea C strain (including degenerative morphological changes, detachment, membrane degradation and death of host cells), were reflected by drastic decrease in impedance signal response detected as early as ~30 hours post-infection (hpi). In contrast, distinct CPE by conventional microscopy was evident only at ~72 hpi at the corresponding multiplicity of infection (MOI) of 10. A parameter that describes the kinetics of cytopathogenesis, CIT50, which refers to the time taken for 50% reduction in impedance signal response, revealed an inverse linear relationship with virus titer and MOI. CIT50 values were also delayed by 31.5 h for each order of magnitude decrease in MOI. Therefore, based on the analysis of CIT50, the virus titer of a given sample can be determined from the measured impedance signal response. Furthermore, consistent impedance results were also obtained with clinical isolates of the four DENV serotypes verified by RT-PCR and cycle sequencing. This impedimetric cell-based biosensor represents a label-free and continuous approach for the dynamic measurement of cellular responses toward DENV infection, and for detecting the presence of infectious viral particles. ASTAR (Agency for Sci., Tech. and Research, S’pore) 2016-07-01T08:45:23Z 2019-12-06T14:35:19Z 2016-07-01T08:45:23Z 2019-12-06T14:35:19Z 2015 Journal Article Cheng, M. S., Lau, S. H., Chan, K. P., Toh, C.-S., & Chow, V. T. (2015). Impedimetric cell-based biosensor for real-time monitoring of cytopathic effects induced by dengue viruses. Biosensors and Bioelectronics, 70, 74-80. 0956-5663 https://hdl.handle.net/10356/81633 http://hdl.handle.net/10220/40877 10.1016/j.bios.2015.03.018 en Biosensors and Bioelectronics © 2015 Elsevier.
institution Nanyang Technological University
building NTU Library
country Singapore
collection DR-NTU
language English
topic Cytopathic effects
Dengue virus serotypes
spellingShingle Cytopathic effects
Dengue virus serotypes
Cheng, Ming Soon
Lau, Suk Hiang
Chan, Kwai Peng
Toh, Chee-Seng
Chow, Vincent T.
Impedimetric cell-based biosensor for real-time monitoring of cytopathic effects induced by dengue viruses
description We describe an impedimetric cell-based biosensor constructed from poly-l-lysine (PLL)-modified screen-printed carbon electrode for real-time monitoring of dengue virus (DENV) infection of surface-immobilized baby hamster kidney (BHK-21) fibroblast cells. Cytopathic effects (CPE) induced by DENV-2 New Guinea C strain (including degenerative morphological changes, detachment, membrane degradation and death of host cells), were reflected by drastic decrease in impedance signal response detected as early as ~30 hours post-infection (hpi). In contrast, distinct CPE by conventional microscopy was evident only at ~72 hpi at the corresponding multiplicity of infection (MOI) of 10. A parameter that describes the kinetics of cytopathogenesis, CIT50, which refers to the time taken for 50% reduction in impedance signal response, revealed an inverse linear relationship with virus titer and MOI. CIT50 values were also delayed by 31.5 h for each order of magnitude decrease in MOI. Therefore, based on the analysis of CIT50, the virus titer of a given sample can be determined from the measured impedance signal response. Furthermore, consistent impedance results were also obtained with clinical isolates of the four DENV serotypes verified by RT-PCR and cycle sequencing. This impedimetric cell-based biosensor represents a label-free and continuous approach for the dynamic measurement of cellular responses toward DENV infection, and for detecting the presence of infectious viral particles.
author2 School of Physical and Mathematical Sciences
author_facet School of Physical and Mathematical Sciences
Cheng, Ming Soon
Lau, Suk Hiang
Chan, Kwai Peng
Toh, Chee-Seng
Chow, Vincent T.
format Article
author Cheng, Ming Soon
Lau, Suk Hiang
Chan, Kwai Peng
Toh, Chee-Seng
Chow, Vincent T.
author_sort Cheng, Ming Soon
title Impedimetric cell-based biosensor for real-time monitoring of cytopathic effects induced by dengue viruses
title_short Impedimetric cell-based biosensor for real-time monitoring of cytopathic effects induced by dengue viruses
title_full Impedimetric cell-based biosensor for real-time monitoring of cytopathic effects induced by dengue viruses
title_fullStr Impedimetric cell-based biosensor for real-time monitoring of cytopathic effects induced by dengue viruses
title_full_unstemmed Impedimetric cell-based biosensor for real-time monitoring of cytopathic effects induced by dengue viruses
title_sort impedimetric cell-based biosensor for real-time monitoring of cytopathic effects induced by dengue viruses
publishDate 2016
url https://hdl.handle.net/10356/81633
http://hdl.handle.net/10220/40877
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