Lab-on-a-chip for rapid electrochemical detection of nerve agent Sarin

This paper reports a lab-on-a-chip for the detection of Sarin nerve agent based on rapid electrochemical detection. The chemical warfare agent Sarin (C4H10FO2P, O-isopropyl methylphosphonofluoridate) is a highly toxic organophosphate that induces rapid respiratory depression, seizures and death with...

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Main Authors: Tan, Hsih Yin, Loke, Weng Keong, Nguyen, Nam-Trung, Tan, Swee Ngin, Tay, Nam Beng, Wang, Wei, Ng, Sum Huan
Other Authors: A*STAR SIMTech
Format: Article
Language:English
Published: 2016
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Online Access:https://hdl.handle.net/10356/81643
http://hdl.handle.net/10220/40876
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Institution: Nanyang Technological University
Language: English
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spelling sg-ntu-dr.10356-816432020-03-07T12:47:12Z Lab-on-a-chip for rapid electrochemical detection of nerve agent Sarin Tan, Hsih Yin Loke, Weng Keong Nguyen, Nam-Trung Tan, Swee Ngin Tay, Nam Beng Wang, Wei Ng, Sum Huan A*STAR SIMTech Lab-on-a-chip Microfluidics This paper reports a lab-on-a-chip for the detection of Sarin nerve agent based on rapid electrochemical detection. The chemical warfare agent Sarin (C4H10FO2P, O-isopropyl methylphosphonofluoridate) is a highly toxic organophosphate that induces rapid respiratory depression, seizures and death within minutes of inhalation. As purified Sarin is colourless, odourless, water soluble and a easily disseminated nerve agent, it has been used as a weapon in terrorist or military attacks. To ascertain whether potable water supplies have been adulterated with this extremely potent poison, an inexpensive, sensitive and easy to use portable test kit would be of interest to first responders investigating such attacks. We report here an amperometric-based approach for detecting trace amounts of Sarin in water samples using a screen-printed electrode (SPE) integrated in a microfluidic chip. Enzymatic inhibition was obtained by exposing the immobilised biosensor in the microfluidic platform to Sarin in water samples. With the aid of cobalt phthalocyanine modified SPE, the device could detect Sarin at part-per-billion levels with concentration as low as 1 nM. The detection method reported here represents a significant improvement over the authors’previous optical-based detection method. ASTAR (Agency for Sci., Tech. and Research, S’pore) 2016-07-01T08:16:51Z 2019-12-06T14:35:27Z 2016-07-01T08:16:51Z 2019-12-06T14:35:27Z 2013 Journal Article Tan, H. Y., Loke, W. K., Nguyen, N.-T., Tan, S. N., Tay, N. B., Wang, W., et al. (2014). Lab-on-a-chip for rapid electrochemical detection of nerve agent Sarin. Biomedical Microdevices, 16(2), 269-275. 1387-2176 https://hdl.handle.net/10356/81643 http://hdl.handle.net/10220/40876 10.1007/s10544-013-9830-4 en Biomedical Microdevices © 2013 Springer Science+Business Media New York.
institution Nanyang Technological University
building NTU Library
country Singapore
collection DR-NTU
language English
topic Lab-on-a-chip
Microfluidics
spellingShingle Lab-on-a-chip
Microfluidics
Tan, Hsih Yin
Loke, Weng Keong
Nguyen, Nam-Trung
Tan, Swee Ngin
Tay, Nam Beng
Wang, Wei
Ng, Sum Huan
Lab-on-a-chip for rapid electrochemical detection of nerve agent Sarin
description This paper reports a lab-on-a-chip for the detection of Sarin nerve agent based on rapid electrochemical detection. The chemical warfare agent Sarin (C4H10FO2P, O-isopropyl methylphosphonofluoridate) is a highly toxic organophosphate that induces rapid respiratory depression, seizures and death within minutes of inhalation. As purified Sarin is colourless, odourless, water soluble and a easily disseminated nerve agent, it has been used as a weapon in terrorist or military attacks. To ascertain whether potable water supplies have been adulterated with this extremely potent poison, an inexpensive, sensitive and easy to use portable test kit would be of interest to first responders investigating such attacks. We report here an amperometric-based approach for detecting trace amounts of Sarin in water samples using a screen-printed electrode (SPE) integrated in a microfluidic chip. Enzymatic inhibition was obtained by exposing the immobilised biosensor in the microfluidic platform to Sarin in water samples. With the aid of cobalt phthalocyanine modified SPE, the device could detect Sarin at part-per-billion levels with concentration as low as 1 nM. The detection method reported here represents a significant improvement over the authors’previous optical-based detection method.
author2 A*STAR SIMTech
author_facet A*STAR SIMTech
Tan, Hsih Yin
Loke, Weng Keong
Nguyen, Nam-Trung
Tan, Swee Ngin
Tay, Nam Beng
Wang, Wei
Ng, Sum Huan
format Article
author Tan, Hsih Yin
Loke, Weng Keong
Nguyen, Nam-Trung
Tan, Swee Ngin
Tay, Nam Beng
Wang, Wei
Ng, Sum Huan
author_sort Tan, Hsih Yin
title Lab-on-a-chip for rapid electrochemical detection of nerve agent Sarin
title_short Lab-on-a-chip for rapid electrochemical detection of nerve agent Sarin
title_full Lab-on-a-chip for rapid electrochemical detection of nerve agent Sarin
title_fullStr Lab-on-a-chip for rapid electrochemical detection of nerve agent Sarin
title_full_unstemmed Lab-on-a-chip for rapid electrochemical detection of nerve agent Sarin
title_sort lab-on-a-chip for rapid electrochemical detection of nerve agent sarin
publishDate 2016
url https://hdl.handle.net/10356/81643
http://hdl.handle.net/10220/40876
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