Studying forkhead box protein A1–DNA interaction and ligand inhibition using gold nanoparticles, electrophoretic mobility shift assay, and fluorescence anisotropy

• Main Authors: Aung, Khin Moh Moh, New, Siu Yee, Hong, Shuzhen, Sutarlie, Laura, Lim, Michelle Gek Liang, Tan, Si Kee, Cheung, Edwin, Su, Xiaodi
• Other Authors: School of Biological Sciences
• Format: Article
• Language: English
• Published: 2016
• Subjects: Gold nanoparticles; Protein–DNA interactions
• Online Access: https://hdl.handle.net/10356/81648; http://hdl.handle.net/10220/40871
• Institution: Nanyang Technological University

Forkhead box protein 1 (FoxA1) is a member of the forkhead family of winged helix transcription factors that plays pivotal roles in the development and differentiation of multiple organs and in the regulation of estrogen-stimulated genes. Conventional analytical methods—electrophoretic mobility shif...