Apelin Enhances Brown Adipogenesis and Browning of White Adipocytes

Brown adipose tissue expends energy in the form of heat via the mitochondrial uncoupling protein UCP1. Recent studies showed that brown adipose tissue is present in adult humans and may be exploited for its anti-obesity and anti-diabetes actions. Apelin is an adipocyte-derived hormone that plays imp...

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Main Authors: Than, Aung, He, Hui Ling, Chua, Si Hui, Xu, Dan, Sun, Lei, Leow, Melvin Khee-Shing, Chen, Peng
Other Authors: School of Chemical and Biomedical Engineering
Format: Article
Language:English
Published: 2016
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Online Access:https://hdl.handle.net/10356/81839
http://hdl.handle.net/10220/39721
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Institution: Nanyang Technological University
Language: English
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spelling sg-ntu-dr.10356-818392022-02-16T16:28:09Z Apelin Enhances Brown Adipogenesis and Browning of White Adipocytes Than, Aung He, Hui Ling Chua, Si Hui Xu, Dan Sun, Lei Leow, Melvin Khee-Shing Chen, Peng School of Chemical and Biomedical Engineering Adipocyte Adipose tissue Browning Uncoupling protein 1 Metabolic Diseases Apelin Brown adipose tissue expends energy in the form of heat via the mitochondrial uncoupling protein UCP1. Recent studies showed that brown adipose tissue is present in adult humans and may be exploited for its anti-obesity and anti-diabetes actions. Apelin is an adipocyte-derived hormone that plays important roles in energy metabolism. Here, we report that apelin-APJ signalling promotes brown adipocyte differentiation, by increasing the expressions of brown adipogenic and thermogenic transcriptional factors via PI3kinase/Akt and AMPK signalling pathways. It is also found that apelin relieves the TNFα inhibition on brown adipogenesis. In addition, apelin increases the basal activity of brown adipocytes, as evidenced by the increased PGC1α and UCP1 expressions, mitochondrial biogenesis, and oxygen consumption. Finally, we provide both in vitro and in vivo evidence that apelin is able to increase the brown-like characteristics in white adipocytes. This study, for the first time, reveals the brown adipogenic and browning effects of apelin, and suggests a potential therapeutic route to combat obesity and related metabolic disorders. NMRC (Natl Medical Research Council, S’pore) Accepted version 2016-01-20T08:24:45Z 2019-12-06T14:41:16Z 2016-01-20T08:24:45Z 2019-12-06T14:41:16Z 2015 Journal Article Than, A., He, H. L., Chua, S. H., Xu, D., Sun, L., Leow, M. K.-S., et al. (2015). Apelin Enhances Brown Adipogenesis and Browning of White Adipocytes. Journal of Biological Chemistry, 290(23), 14679-. 0021-9258 https://hdl.handle.net/10356/81839 http://hdl.handle.net/10220/39721 10.1074/jbc.M115.643817 25931124 en Journal of Biological Chemistry © 2015 American Society for Biochemistry and Molecular Biology (ASBMB). This is the author created version of a work that has been peer reviewed and accepted for publication by Journal of Biological Chemistry, American Society for Biochemistry and Molecular Biology (ASBMB). It incorporates referee’s comments but changes resulting from the publishing process, such as copyediting, structural formatting, may not be reflected in this document. The published version is available at: [http://dx.doi.org/10.1074/jbc.M115.643817]. 24 p. application/msword
institution Nanyang Technological University
building NTU Library
continent Asia
country Singapore
Singapore
content_provider NTU Library
collection DR-NTU
language English
topic Adipocyte
Adipose tissue
Browning
Uncoupling protein 1
Metabolic Diseases
Apelin
spellingShingle Adipocyte
Adipose tissue
Browning
Uncoupling protein 1
Metabolic Diseases
Apelin
Than, Aung
He, Hui Ling
Chua, Si Hui
Xu, Dan
Sun, Lei
Leow, Melvin Khee-Shing
Chen, Peng
Apelin Enhances Brown Adipogenesis and Browning of White Adipocytes
description Brown adipose tissue expends energy in the form of heat via the mitochondrial uncoupling protein UCP1. Recent studies showed that brown adipose tissue is present in adult humans and may be exploited for its anti-obesity and anti-diabetes actions. Apelin is an adipocyte-derived hormone that plays important roles in energy metabolism. Here, we report that apelin-APJ signalling promotes brown adipocyte differentiation, by increasing the expressions of brown adipogenic and thermogenic transcriptional factors via PI3kinase/Akt and AMPK signalling pathways. It is also found that apelin relieves the TNFα inhibition on brown adipogenesis. In addition, apelin increases the basal activity of brown adipocytes, as evidenced by the increased PGC1α and UCP1 expressions, mitochondrial biogenesis, and oxygen consumption. Finally, we provide both in vitro and in vivo evidence that apelin is able to increase the brown-like characteristics in white adipocytes. This study, for the first time, reveals the brown adipogenic and browning effects of apelin, and suggests a potential therapeutic route to combat obesity and related metabolic disorders.
author2 School of Chemical and Biomedical Engineering
author_facet School of Chemical and Biomedical Engineering
Than, Aung
He, Hui Ling
Chua, Si Hui
Xu, Dan
Sun, Lei
Leow, Melvin Khee-Shing
Chen, Peng
format Article
author Than, Aung
He, Hui Ling
Chua, Si Hui
Xu, Dan
Sun, Lei
Leow, Melvin Khee-Shing
Chen, Peng
author_sort Than, Aung
title Apelin Enhances Brown Adipogenesis and Browning of White Adipocytes
title_short Apelin Enhances Brown Adipogenesis and Browning of White Adipocytes
title_full Apelin Enhances Brown Adipogenesis and Browning of White Adipocytes
title_fullStr Apelin Enhances Brown Adipogenesis and Browning of White Adipocytes
title_full_unstemmed Apelin Enhances Brown Adipogenesis and Browning of White Adipocytes
title_sort apelin enhances brown adipogenesis and browning of white adipocytes
publishDate 2016
url https://hdl.handle.net/10356/81839
http://hdl.handle.net/10220/39721
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