Optimization of calcium-based bioclogging and biocementation of sand

Bioclogging and biocementation can be used to improve the geotechnical properties of sand. These processes can be performed by adsorption of urease-producing bacterial cells on the sand grain surfaces, which is followed by crystallization of calcite produced from the calcium salt and urea solution d...

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Main Authors: Chu, Jian, Ivanov, Volodymyr, Naeimi, Maryam, Stabnikov, Viktor, Liu, Han-Long
Other Authors: School of Civil and Environmental Engineering
Format: Article
Language:English
Published: 2016
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Online Access:https://hdl.handle.net/10356/81878
http://hdl.handle.net/10220/39693
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Institution: Nanyang Technological University
Language: English
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spelling sg-ntu-dr.10356-818782020-03-07T11:43:30Z Optimization of calcium-based bioclogging and biocementation of sand Chu, Jian Ivanov, Volodymyr Naeimi, Maryam Stabnikov, Viktor Liu, Han-Long School of Civil and Environmental Engineering Biogrouting Bioclogging Sand Biocementation Bioclogging and biocementation can be used to improve the geotechnical properties of sand. These processes can be performed by adsorption of urease-producing bacterial cells on the sand grain surfaces, which is followed by crystallization of calcite produced from the calcium salt and urea solution due to bacterial hydrolysis of urea. In this paper, the effect of intact cell suspension of Bacillus sp. strain VS1, suspension of the washed bacterial cells, and culture liquid without bacterial cells on microbially induced calcite precipitation in sand was studied. The test results showed that adsorption/retention of urease activity on sand treated with washed cells of Bacillus sp. strain VS1 was 5–8 times higher than that treated with culture liquid. The unconfined compressive strength of sand treated with the suspension of washed cells was 1.7 times higher than that treated with culture liquid. This difference could be due to fast inactivation of urease by protease which was present in the culture liquid. The adsorption of bacterial cells on sand pretreated with calcium, aluminum, or ferric salts was 29–37 % higher as compared with that without pretreatment. The permeability of sand varied with the content of precipitated calcium. For bioclogging of sand, the content of precipitated calcium had to be 1.3 % (w/w) or higher. The shear strength of biotreated sand was also dependent on the content of precipitated calcium. To achieve an unconfined compressive strength of 1.5 MPa or higher, the content of precipitated calcium in the treated sand had to be 4.2 % (w/w) or higher. These data can be used as the reference values for geotechnical applications such as bioclogging for reducing the permeability of sand and biocementation for increasing the shear strength of soil. ASTAR (Agency for Sci., Tech. and Research, S’pore) Accepted version 2016-01-13T07:04:53Z 2019-12-06T14:42:10Z 2016-01-13T07:04:53Z 2019-12-06T14:42:10Z 2013 Journal Article Chu, J., Ivanov, V., Naeimi, M., Stabnikov, V., & Liu, H.-L. (2014). Optimization of calcium-based bioclogging and biocementation of sand. Acta Geotechnica, 9(2), 277-285. 1861-1125 https://hdl.handle.net/10356/81878 http://hdl.handle.net/10220/39693 10.1007/s11440-013-0278-8 en Acta Geotechnica © 2013 Springer-Verlag Berlin Heidelberg. This is the author created version of a work that has been peer reviewed and accepted for publication by Acta Geotechnica, Springer-Verlag Berlin Heidelberg. It incorporates referee’s comments but changes resulting from the publishing process, such as copyediting, structural formatting, may not be reflected in this document. The published version is available at: [http://dx.doi.org/10.1007/s11440-013-0278-8]. 17 p. application/pdf
institution Nanyang Technological University
building NTU Library
country Singapore
collection DR-NTU
language English
topic Biogrouting
Bioclogging
Sand
Biocementation
spellingShingle Biogrouting
Bioclogging
Sand
Biocementation
Chu, Jian
Ivanov, Volodymyr
Naeimi, Maryam
Stabnikov, Viktor
Liu, Han-Long
Optimization of calcium-based bioclogging and biocementation of sand
description Bioclogging and biocementation can be used to improve the geotechnical properties of sand. These processes can be performed by adsorption of urease-producing bacterial cells on the sand grain surfaces, which is followed by crystallization of calcite produced from the calcium salt and urea solution due to bacterial hydrolysis of urea. In this paper, the effect of intact cell suspension of Bacillus sp. strain VS1, suspension of the washed bacterial cells, and culture liquid without bacterial cells on microbially induced calcite precipitation in sand was studied. The test results showed that adsorption/retention of urease activity on sand treated with washed cells of Bacillus sp. strain VS1 was 5–8 times higher than that treated with culture liquid. The unconfined compressive strength of sand treated with the suspension of washed cells was 1.7 times higher than that treated with culture liquid. This difference could be due to fast inactivation of urease by protease which was present in the culture liquid. The adsorption of bacterial cells on sand pretreated with calcium, aluminum, or ferric salts was 29–37 % higher as compared with that without pretreatment. The permeability of sand varied with the content of precipitated calcium. For bioclogging of sand, the content of precipitated calcium had to be 1.3 % (w/w) or higher. The shear strength of biotreated sand was also dependent on the content of precipitated calcium. To achieve an unconfined compressive strength of 1.5 MPa or higher, the content of precipitated calcium in the treated sand had to be 4.2 % (w/w) or higher. These data can be used as the reference values for geotechnical applications such as bioclogging for reducing the permeability of sand and biocementation for increasing the shear strength of soil.
author2 School of Civil and Environmental Engineering
author_facet School of Civil and Environmental Engineering
Chu, Jian
Ivanov, Volodymyr
Naeimi, Maryam
Stabnikov, Viktor
Liu, Han-Long
format Article
author Chu, Jian
Ivanov, Volodymyr
Naeimi, Maryam
Stabnikov, Viktor
Liu, Han-Long
author_sort Chu, Jian
title Optimization of calcium-based bioclogging and biocementation of sand
title_short Optimization of calcium-based bioclogging and biocementation of sand
title_full Optimization of calcium-based bioclogging and biocementation of sand
title_fullStr Optimization of calcium-based bioclogging and biocementation of sand
title_full_unstemmed Optimization of calcium-based bioclogging and biocementation of sand
title_sort optimization of calcium-based bioclogging and biocementation of sand
publishDate 2016
url https://hdl.handle.net/10356/81878
http://hdl.handle.net/10220/39693
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