Halotolerant, alkaliphilic urease-producing bacteria from different climate zones and their application for biocementation of sand

Microbially induced calcium carbonate precipitation (MICP) is a phenomenon based on urease activity of halotolerant and alkaliphilic microorganisms that can be used for the soil bioclogging and biocementation in geotechnical engineering. However, enrichment cultures produced from indigenous soil bac...

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Main Authors: Stabnikov, Viktor, Jian, Chu, Ivanov, Volodymyr, Li, Yishan
Other Authors: School of Civil and Environmental Engineering
Format: Article
Language:English
Published: 2016
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Online Access:https://hdl.handle.net/10356/81879
http://hdl.handle.net/10220/39692
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Institution: Nanyang Technological University
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spelling sg-ntu-dr.10356-818792020-03-07T11:43:30Z Halotolerant, alkaliphilic urease-producing bacteria from different climate zones and their application for biocementation of sand Stabnikov, Viktor Jian, Chu Ivanov, Volodymyr Li, Yishan School of Civil and Environmental Engineering Climate zones Urease-producing bacteria Biocementation Microbially induced calcium carbonate precipitation (MICP) is a phenomenon based on urease activity of halotolerant and alkaliphilic microorganisms that can be used for the soil bioclogging and biocementation in geotechnical engineering. However, enrichment cultures produced from indigenous soil bacteria cannot be used for large-scale MICP because their urease activity decreased with the rate about 5 % per one generation. To ensure stability of urease activity in biocement, halotolerant and alkaliphilic strains of urease-producing bacteria for soil biocementation were isolated from either sandy soil or high salinity water in different climate zones. The strain Bacillus sp. VUK5, isolated from soil in Ukraine (continental climate), was phylogenetically close in identity (99 % of 16S rRNA gene sequence) to the strain of Bacillus sp. VS1 isolated from beach sand in Singapore (tropical rainforest climate), as well as to the strains of Bacillus sp. isolated by other researchers in Ghent, Belgium (maritime temperate climate) and Yogyakarta, Indonesia (tropical rainforest climate). Both strains Bacillus sp. VS1 and VUK5 had maximum specific growth rate of 0.09/h and maximum urease activities of 6.2 and 8.8 mM of hydrolysed urea/min, respectively. The halotolerant and alkaliphilic strain of urease-producing bacteria isolated from water of the saline lake Dead Sea in Jordan was presented by Gram-positive cocci close to the species Staphylococcus succinus. However, the strains of this species could be hemolytic and toxigenic, therefore only representatives of alkaliphilic Bacillus sp. were used for the biocementation studies. Unconfined compressive strengths for dry biocemented sand samples after six batch treatments with strains VS1and VUK5 were 765 and 845 kPa, respectively. The content of precipitated calcium and the strength of dry biocemented sand at permeability equals to 1 % of initial value were 12.4 g Ca/kg of dry sand and 454 kPa, respectively, in case of biocementation by the strain VS1. So, halotolerant, alkaliphilic, urease-producing bacteria isolated from different climate zones have similar properties and can be used for biocementation of soil. ASTAR (Agency for Sci., Tech. and Research, S’pore) Accepted version 2016-01-13T07:03:12Z 2019-12-06T14:42:12Z 2016-01-13T07:03:12Z 2019-12-06T14:42:12Z 2013 Journal Article Stabnikov, V., Jian, C., Ivanov, V., & Li, Y. (2013). Halotolerant, alkaliphilic urease-producing bacteria from different climate zones and their application for biocementation of sand. World Journal of Microbiology and Biotechnology, 29(8), 1453-1460. 0959-3993 https://hdl.handle.net/10356/81879 http://hdl.handle.net/10220/39692 10.1007/s11274-013-1309-1 en World Journal of Microbiology and Biotechnology © 2013 Springer Science+Business Media Dordrecht. This is the author created version of a work that has been peer reviewed and accepted for publication by World Journal of Microbiology and Biotechnology, Springer Science+Business Media Dordrecht. It incorporates referee’s comments but changes resulting from the publishing process, such as copyediting, structural formatting, may not be reflected in this document. The published version is available at: [http://dx.doi.org/10.1007/s11274-013-1309-1]. 22 p. application/pdf
institution Nanyang Technological University
building NTU Library
country Singapore
collection DR-NTU
language English
topic Climate zones
Urease-producing bacteria
Biocementation
spellingShingle Climate zones
Urease-producing bacteria
Biocementation
Stabnikov, Viktor
Jian, Chu
Ivanov, Volodymyr
Li, Yishan
Halotolerant, alkaliphilic urease-producing bacteria from different climate zones and their application for biocementation of sand
description Microbially induced calcium carbonate precipitation (MICP) is a phenomenon based on urease activity of halotolerant and alkaliphilic microorganisms that can be used for the soil bioclogging and biocementation in geotechnical engineering. However, enrichment cultures produced from indigenous soil bacteria cannot be used for large-scale MICP because their urease activity decreased with the rate about 5 % per one generation. To ensure stability of urease activity in biocement, halotolerant and alkaliphilic strains of urease-producing bacteria for soil biocementation were isolated from either sandy soil or high salinity water in different climate zones. The strain Bacillus sp. VUK5, isolated from soil in Ukraine (continental climate), was phylogenetically close in identity (99 % of 16S rRNA gene sequence) to the strain of Bacillus sp. VS1 isolated from beach sand in Singapore (tropical rainforest climate), as well as to the strains of Bacillus sp. isolated by other researchers in Ghent, Belgium (maritime temperate climate) and Yogyakarta, Indonesia (tropical rainforest climate). Both strains Bacillus sp. VS1 and VUK5 had maximum specific growth rate of 0.09/h and maximum urease activities of 6.2 and 8.8 mM of hydrolysed urea/min, respectively. The halotolerant and alkaliphilic strain of urease-producing bacteria isolated from water of the saline lake Dead Sea in Jordan was presented by Gram-positive cocci close to the species Staphylococcus succinus. However, the strains of this species could be hemolytic and toxigenic, therefore only representatives of alkaliphilic Bacillus sp. were used for the biocementation studies. Unconfined compressive strengths for dry biocemented sand samples after six batch treatments with strains VS1and VUK5 were 765 and 845 kPa, respectively. The content of precipitated calcium and the strength of dry biocemented sand at permeability equals to 1 % of initial value were 12.4 g Ca/kg of dry sand and 454 kPa, respectively, in case of biocementation by the strain VS1. So, halotolerant, alkaliphilic, urease-producing bacteria isolated from different climate zones have similar properties and can be used for biocementation of soil.
author2 School of Civil and Environmental Engineering
author_facet School of Civil and Environmental Engineering
Stabnikov, Viktor
Jian, Chu
Ivanov, Volodymyr
Li, Yishan
format Article
author Stabnikov, Viktor
Jian, Chu
Ivanov, Volodymyr
Li, Yishan
author_sort Stabnikov, Viktor
title Halotolerant, alkaliphilic urease-producing bacteria from different climate zones and their application for biocementation of sand
title_short Halotolerant, alkaliphilic urease-producing bacteria from different climate zones and their application for biocementation of sand
title_full Halotolerant, alkaliphilic urease-producing bacteria from different climate zones and their application for biocementation of sand
title_fullStr Halotolerant, alkaliphilic urease-producing bacteria from different climate zones and their application for biocementation of sand
title_full_unstemmed Halotolerant, alkaliphilic urease-producing bacteria from different climate zones and their application for biocementation of sand
title_sort halotolerant, alkaliphilic urease-producing bacteria from different climate zones and their application for biocementation of sand
publishDate 2016
url https://hdl.handle.net/10356/81879
http://hdl.handle.net/10220/39692
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