Cloning, expression, purification and crystallization of a pair of novel virulence factors, SghA and SghR, from Agrobacterium tumefaciens

• Main Authors: Ye, Fuzhou, Wang, Chao, Fu, Qinqin, Zhang, Lian-hui, Gao, Yong-Gui
• Other Authors: School of Biological Sciences
• Format: Article
• Language: English
• Published: 2016
• Subjects: SghR; SghA
• Online Access: https://hdl.handle.net/10356/82098; http://hdl.handle.net/10220/41125
• Institution: Nanyang Technological University

Two proteins, SghA and SghR, which were recently identified and characterized as novel bacterial virulence factors regulating the infection of plant hosts by Agrobacterium, were cloned, overexpressed and purified with high yield. Both SghA and SghR form dimers in solution. The purified SghA and SghR...