Slr0151 in Synechocystis sp. PCC 6803 is required for efficient repair of photosystem II under high-light condition

Cyanobacteria are ancient photosynthetic prokaryotes that have adapted successfully to adverse environments including high-light irradiation. Although it is known that the repair of photodamaged photosystem II (PSII) in the organisms is a highly regulated process, our knowledge of the molecular comp...

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Main Authors: Yang, Haomeng, Liao, Libing, Bo, Tingting, Zhao, Lei, Sun, Xuwu, Lu, Xuefeng, Norling, Birgitta, Huang, Fang
Other Authors: School of Biological Sciences
Format: Article
Language:English
Published: 2016
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Online Access:https://hdl.handle.net/10356/82150
http://hdl.handle.net/10220/41133
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Institution: Nanyang Technological University
Language: English
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spelling sg-ntu-dr.10356-821502023-02-28T17:00:49Z Slr0151 in Synechocystis sp. PCC 6803 is required for efficient repair of photosystem II under high-light condition Yang, Haomeng Liao, Libing Bo, Tingting Zhao, Lei Sun, Xuwu Lu, Xuefeng Norling, Birgitta Huang, Fang School of Biological Sciences Slr0151 Synechocystis Cyanobacteria are ancient photosynthetic prokaryotes that have adapted successfully to adverse environments including high-light irradiation. Although it is known that the repair of photodamaged photosystem II (PSII) in the organisms is a highly regulated process, our knowledge of the molecular components that regulate each step of the process is limited. We have previously identified a hypothetical protein Slr0151 in the membrane fractions of cyanobacterium Synechocystis sp. PCC 6803. Here, we report that Slr0151 is involved in PSII repair of the organism. We generated a mutant strain (Δslr0151) lacking the protein Slr0151 and analyzed its characteristics under normal and high-light conditions. Targeted deletion of slr0151 resulted in decreased PSII activity in Synechocystis. Moreover, the mutant exhibited increased photoinhibition due to impairment of PSII repair under high-light condition. Further analysis using in vivo radioactive labeling and 2-D blue native/sodium dodecylsulfate polyacrylamide gel electrophoresis indicated that the PSII repair cycle was hindered at the levels of D1 synthesis and disassembly and/or assembly of PSII in the mutant. Protein interaction assays demonstrated that Slr0151 interacts with D1 and CP43 proteins. Taken together, these results indicate that Slr0151 plays an important role in regulating PSII repair in the organism under high-light stress condition. Published version 2016-08-15T08:51:05Z 2019-12-06T14:47:37Z 2016-08-15T08:51:05Z 2019-12-06T14:47:37Z 2014 Journal Article Yang, H., Liao, L., Bo, T., Zhao, L., Sun, X., Lu, X., et al. (2014). Slr0151 in Synechocystis sp. PCC 6803 is required for efficient repair of photosystem II under high-light condition. Journal of Integrative Plant Biology, 56(12), 1136-1150. 1672-9072 https://hdl.handle.net/10356/82150 http://hdl.handle.net/10220/41133 10.1111/jipb.12275 en Journal of Integrative Plant Biology © 2014 Institute of Botany, Chinese Academy of Sciences. This paper was published in Journal of Integrative Plant Biology and is made available as an electronic reprint (preprint) with permission of Institute of Botany, Chinese Academy of Sciences. The published version is available at: [http://dx.doi.org/10.1111/jipb.12275]. One print or electronic copy may be made for personal use only. Systematic or multiple reproduction, distribution to multiple locations via electronic or other means, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper is prohibited and is subject to penalties under law. 15 p. application/pdf
institution Nanyang Technological University
building NTU Library
continent Asia
country Singapore
Singapore
content_provider NTU Library
collection DR-NTU
language English
topic Slr0151
Synechocystis
spellingShingle Slr0151
Synechocystis
Yang, Haomeng
Liao, Libing
Bo, Tingting
Zhao, Lei
Sun, Xuwu
Lu, Xuefeng
Norling, Birgitta
Huang, Fang
Slr0151 in Synechocystis sp. PCC 6803 is required for efficient repair of photosystem II under high-light condition
description Cyanobacteria are ancient photosynthetic prokaryotes that have adapted successfully to adverse environments including high-light irradiation. Although it is known that the repair of photodamaged photosystem II (PSII) in the organisms is a highly regulated process, our knowledge of the molecular components that regulate each step of the process is limited. We have previously identified a hypothetical protein Slr0151 in the membrane fractions of cyanobacterium Synechocystis sp. PCC 6803. Here, we report that Slr0151 is involved in PSII repair of the organism. We generated a mutant strain (Δslr0151) lacking the protein Slr0151 and analyzed its characteristics under normal and high-light conditions. Targeted deletion of slr0151 resulted in decreased PSII activity in Synechocystis. Moreover, the mutant exhibited increased photoinhibition due to impairment of PSII repair under high-light condition. Further analysis using in vivo radioactive labeling and 2-D blue native/sodium dodecylsulfate polyacrylamide gel electrophoresis indicated that the PSII repair cycle was hindered at the levels of D1 synthesis and disassembly and/or assembly of PSII in the mutant. Protein interaction assays demonstrated that Slr0151 interacts with D1 and CP43 proteins. Taken together, these results indicate that Slr0151 plays an important role in regulating PSII repair in the organism under high-light stress condition.
author2 School of Biological Sciences
author_facet School of Biological Sciences
Yang, Haomeng
Liao, Libing
Bo, Tingting
Zhao, Lei
Sun, Xuwu
Lu, Xuefeng
Norling, Birgitta
Huang, Fang
format Article
author Yang, Haomeng
Liao, Libing
Bo, Tingting
Zhao, Lei
Sun, Xuwu
Lu, Xuefeng
Norling, Birgitta
Huang, Fang
author_sort Yang, Haomeng
title Slr0151 in Synechocystis sp. PCC 6803 is required for efficient repair of photosystem II under high-light condition
title_short Slr0151 in Synechocystis sp. PCC 6803 is required for efficient repair of photosystem II under high-light condition
title_full Slr0151 in Synechocystis sp. PCC 6803 is required for efficient repair of photosystem II under high-light condition
title_fullStr Slr0151 in Synechocystis sp. PCC 6803 is required for efficient repair of photosystem II under high-light condition
title_full_unstemmed Slr0151 in Synechocystis sp. PCC 6803 is required for efficient repair of photosystem II under high-light condition
title_sort slr0151 in synechocystis sp. pcc 6803 is required for efficient repair of photosystem ii under high-light condition
publishDate 2016
url https://hdl.handle.net/10356/82150
http://hdl.handle.net/10220/41133
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