Going native: Complete removal of protein purification affinity tags by simple modification of existing tags and proteases

• Main Authors: Goh, Hui Chin, Sobota, Radoslaw M., Ghadessy, Farid J., Nirantar, Saurabh
• Other Authors: School of Biological Sciences
• Format: Article
• Language: English
• Published: 2017
• Subjects: Protein purification; Affinity tags; Protease
• Online Access: https://hdl.handle.net/10356/83191; http://hdl.handle.net/10220/42481
• Institution: Nanyang Technological University

Protein purification typically involves expressing a recombinant gene comprising a target protein fused to a suitable affinity tag. After purification, it is often desirable to remove the affinity tag to prevent interference with downstream functions of the target protein. This is mainly accomplishe...