Presence of negative supercoiling in aggregates of histone H1-plasmidic polynucleosome complexes

Presence of negative supercoiling in aggregates of histone H1-plasmidic polynucleosome complexes

It was shown in the past that in the presence of histone H1, plasmidic polynucleosomes formed densely packed aggregates. Our current studies demonstrate that these aggregates are susceptible to the actions of E. coli topoisomerase I, human topoisomerase I and DNA nicking enzyme, which is the indicat...

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Main Authors: Zhang, Hao, Li, Tianhu
Other Authors: School of Physical and Mathematical Sciences
Format: Article
Language:English
Published: 2017
Subjects:
Plasmid DNA
Histone H1
Online Access:https://hdl.handle.net/10356/83232
http://hdl.handle.net/10220/42505
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Institution: Nanyang Technological University
Language: English
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spelling sg-ntu-dr.10356-832322023-02-28T19:32:25Z Presence of negative supercoiling in aggregates of histone H1-plasmidic polynucleosome complexes Zhang, Hao Li, Tianhu School of Physical and Mathematical Sciences Plasmid DNA Histone H1 It was shown in the past that in the presence of histone H1, plasmidic polynucleosomes formed densely packed aggregates. Our current studies demonstrate that these aggregates are susceptible to the actions of E. coli topoisomerase I, human topoisomerase I and DNA nicking enzyme, which is the indication that negative supercoiling is present in the condensed DNA-protein complexes. Since negative supercoiling leads to formation of highly curved and compact plectonemic and toroidal DNA structures, it would be reasonable to assume that DNA negative supercoils are responsible for aggregation of histone H1-plasmidic polynucleosome complexes. Accepted version 2017-05-26T07:04:45Z 2019-12-06T15:17:58Z 2017-05-26T07:04:45Z 2019-12-06T15:17:58Z 2017 Journal Article Zhang, H., & Li, T. (2017). Presence of negative supercoiling in aggregates of histone H1-plasmidic polynucleosome complexes. Bioorganic & Medicinal Chemistry Letters, 27(2), 168-170. 0960-894X https://hdl.handle.net/10356/83232 http://hdl.handle.net/10220/42505 10.1016/j.bmcl.2016.11.088 en Bioorganic & Medicinal Chemistry Letters © 2016 Elsevier Ltd. This is the author created version of a work that has been peer reviewed and accepted for publication by Bioorganic & Medicinal Chemistry Letters, Elsevier. It incorporates referee’s comments but changes resulting from the publishing process, such as copyediting, structural formatting, may not be reflected in this document. The published version is available at: [http://dx.doi.org/10.1016/j.bmcl.2016.11.088]. 4 p. application/pdf
institution Nanyang Technological University
building NTU Library
continent Asia
country Singapore
Singapore
content_provider NTU Library
collection DR-NTU
language English
topic Plasmid DNA
Histone H1
spellingShingle Plasmid DNA
Histone H1
Zhang, Hao
Li, Tianhu
Presence of negative supercoiling in aggregates of histone H1-plasmidic polynucleosome complexes
description It was shown in the past that in the presence of histone H1, plasmidic polynucleosomes formed densely packed aggregates. Our current studies demonstrate that these aggregates are susceptible to the actions of E. coli topoisomerase I, human topoisomerase I and DNA nicking enzyme, which is the indication that negative supercoiling is present in the condensed DNA-protein complexes. Since negative supercoiling leads to formation of highly curved and compact plectonemic and toroidal DNA structures, it would be reasonable to assume that DNA negative supercoils are responsible for aggregation of histone H1-plasmidic polynucleosome complexes.
author2 School of Physical and Mathematical Sciences
author_facet School of Physical and Mathematical Sciences
Zhang, Hao
Li, Tianhu
format Article
author Zhang, Hao
Li, Tianhu
author_sort Zhang, Hao
title Presence of negative supercoiling in aggregates of histone H1-plasmidic polynucleosome complexes
title_short Presence of negative supercoiling in aggregates of histone H1-plasmidic polynucleosome complexes
title_full Presence of negative supercoiling in aggregates of histone H1-plasmidic polynucleosome complexes
title_fullStr Presence of negative supercoiling in aggregates of histone H1-plasmidic polynucleosome complexes
title_full_unstemmed Presence of negative supercoiling in aggregates of histone H1-plasmidic polynucleosome complexes
title_sort presence of negative supercoiling in aggregates of histone h1-plasmidic polynucleosome complexes
publishDate 2017
url https://hdl.handle.net/10356/83232
http://hdl.handle.net/10220/42505
_version_ 1759853639833223168

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