3.9 Å structure of the nucleosome core particle determined by phase-plate cryo-EM

• Main Authors: Chua, Eugene Yue Dao, Vogirala, Vinod Kumar, Inian, Oviya, Wong, Andrew See Weng, Nordenskiold, Lars, Plitzko, Juergen M., Danev, Radostin, Sandin, Sara
• Other Authors: School of Biological Sciences
• Format: Article
• Language: English
• Published: 2016
• Subjects: Nucleosome Core Particle; cryo-EM
• Online Access: https://hdl.handle.net/10356/83403; http://hdl.handle.net/10220/41490
• Institution: Nanyang Technological University

The Volta phase plate is a recently developed electron cryo-microscopy (cryo-EM) device that enables contrast enhancement of biological samples. Here we have evaluated the potential of combining phase-plate imaging and single particle analysis to determine the structure of a small protein–DNA comple...