Identification of novel thermostable ω-transaminase and its application for enzymatic synthesis of chiral amines at high temperature

• Main Authors: Mathew, Sam, Deepankumar, Kanagavel, Shin, Giyoung, Hong, Eun Young, Kim, Byung-Gee, Chung, Taeowan, Yun, Hyungdon
• Other Authors: School of Materials Science & Engineering
• Format: Article
• Language: English
• Published: 2017
• Subjects: Omega-transaminases; Enzymatic synthesis
• Online Access: https://hdl.handle.net/10356/83502; http://hdl.handle.net/10220/42634
• Institution: Nanyang Technological University

A novel thermostable ω-transaminase from Thermomicrobium roseum which showed broad substrate specificity and high enantioselectivity was identified, expressed and biochemically characterized. The advantage of this enzyme to remove volatile inhibitory by-products was demonstrated by performing asymme...