Ribozyme Mediated gRNA Generation for In Vitro and In Vivo CRISPR/Cas9 Mutagenesis

• Main Authors: Lee, Raymond Teck Ho, Ng, Ashley Shu Mei, Ingham, Philip William
• Other Authors: Fujii, Hodaka
• Format: Article
• Language: English
• Published: 2016
• Subjects: Embryos; Ribozymes
• Online Access: https://hdl.handle.net/10356/84342; http://hdl.handle.net/10220/41788
• Institution: Nanyang Technological University

CRISPR/Cas9 is now regularly used for targeted mutagenesis in a wide variety of systems. Here we report the use of ribozymes for the generation of gRNAs both in vitro and in zebrafish embryos. We show that incorporation of ribozymes increases the types of promoters and number of target sites availab...