Cellular Stiffness Measurement for 3D Biological Printing

During 3D biological printing, cells can sense their environment and change their own properties accordingly. In order to understand how cells modulate their stiffness with resp ect to their environmental stiffness, micropipette aspiration method was used to measure the aspiration lengths of porcine...

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Main Authors: Wenna, Badjo, Li, Cheng, Xu, Qinwei, Shreyas, Kuddannaya, Zhang, Yilei, Kang, Yuejun
Other Authors: School of Mechanical and Aerospace Engineering
Format: Conference or Workshop Item
Language:English
Published: 2016
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Online Access:https://hdl.handle.net/10356/84345
http://hdl.handle.net/10220/41742
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Institution: Nanyang Technological University
Language: English
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spelling sg-ntu-dr.10356-843452020-09-24T20:11:46Z Cellular Stiffness Measurement for 3D Biological Printing Wenna, Badjo Li, Cheng Xu, Qinwei Shreyas, Kuddannaya Zhang, Yilei Kang, Yuejun School of Mechanical and Aerospace Engineering Proceedings of the 1st International Conference on Progress in Additive Manufacturing (Pro-AM 2014) Singapore Centre for 3D Printing Singapore Centre for Environmental Life Sciences Engineering Cellular stiffness Substrate effects During 3D biological printing, cells can sense their environment and change their own properties accordingly. In order to understand how cells modulate their stiffness with resp ect to their environmental stiffness, micropipette aspiration method was used to measure the aspiration lengths of porcine mesenchymal stem cells (pMSCs), which were cultured on polydimethylsiloxane (PDMS) substrates with different stiffness for different time periods, under certain pressure. After the measurem ents, both elastic and viscoelastic models were used to analyze the elasticity of the cell. Clear relationship between PDMS stiffness and cell stiffness could not be obtained with elastic model. However, from viscoelastic model, it gives that cells cultured on softest PDMS had the largest elastic modulus while on stiffest PDMS had lowest elastic modulus. Published version 2016-12-07T07:59:17Z 2019-12-06T15:43:12Z 2016-12-07T07:59:17Z 2019-12-06T15:43:12Z 2014 Conference Paper Wenna, B., Li, C., Xu, Q., Shreyas, K., Zhang, Y., & Kang, Y. (2014). Cellular Stiffness Measurement for 3D Biological Printing. Proceedings of the 1st International Conference on Progress in Additive Manufacturing (Pro-AM 2014), 417-422. https://hdl.handle.net/10356/84345 http://hdl.handle.net/10220/41742 10.3850/978-981-09-0446-3_111 en © 2014 by Research Publishing Services. 6 p. application/pdf
institution Nanyang Technological University
building NTU Library
country Singapore
collection DR-NTU
language English
topic Cellular stiffness
Substrate effects
spellingShingle Cellular stiffness
Substrate effects
Wenna, Badjo
Li, Cheng
Xu, Qinwei
Shreyas, Kuddannaya
Zhang, Yilei
Kang, Yuejun
Cellular Stiffness Measurement for 3D Biological Printing
description During 3D biological printing, cells can sense their environment and change their own properties accordingly. In order to understand how cells modulate their stiffness with resp ect to their environmental stiffness, micropipette aspiration method was used to measure the aspiration lengths of porcine mesenchymal stem cells (pMSCs), which were cultured on polydimethylsiloxane (PDMS) substrates with different stiffness for different time periods, under certain pressure. After the measurem ents, both elastic and viscoelastic models were used to analyze the elasticity of the cell. Clear relationship between PDMS stiffness and cell stiffness could not be obtained with elastic model. However, from viscoelastic model, it gives that cells cultured on softest PDMS had the largest elastic modulus while on stiffest PDMS had lowest elastic modulus.
author2 School of Mechanical and Aerospace Engineering
author_facet School of Mechanical and Aerospace Engineering
Wenna, Badjo
Li, Cheng
Xu, Qinwei
Shreyas, Kuddannaya
Zhang, Yilei
Kang, Yuejun
format Conference or Workshop Item
author Wenna, Badjo
Li, Cheng
Xu, Qinwei
Shreyas, Kuddannaya
Zhang, Yilei
Kang, Yuejun
author_sort Wenna, Badjo
title Cellular Stiffness Measurement for 3D Biological Printing
title_short Cellular Stiffness Measurement for 3D Biological Printing
title_full Cellular Stiffness Measurement for 3D Biological Printing
title_fullStr Cellular Stiffness Measurement for 3D Biological Printing
title_full_unstemmed Cellular Stiffness Measurement for 3D Biological Printing
title_sort cellular stiffness measurement for 3d biological printing
publishDate 2016
url https://hdl.handle.net/10356/84345
http://hdl.handle.net/10220/41742
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