Surface plasmon-enhanced fluorescence on Au nanohole array for prostate-specific antigen detection

Localized surface plasmon (LSP) has been widely applied for the enhancement of fluorescence emission for biosensing owing to its potential for strong field enhancement. However, due to its small penetration depth, LSP offers limited fluorescence enhancement over a whole sensor chip and, therefore, i...

Full description

Saved in:
Bibliographic Details
Main Authors: Zhang, Qingwen, Wu, Lin, Wong, Ten It, Zhang, Jinling, Liu, Xiaohu, Zhou, Xiaodong, Bai, Ping, Liedberg, Bo, Wang, Yi
Other Authors: School of Materials Science & Engineering
Format: Article
Language:English
Published: 2017
Subjects:
Online Access:https://hdl.handle.net/10356/84365
http://hdl.handle.net/10220/43589
Tags: Add Tag
No Tags, Be the first to tag this record!
Institution: Nanyang Technological University
Language: English
id sg-ntu-dr.10356-84365
record_format dspace
spelling sg-ntu-dr.10356-843652023-07-14T15:50:59Z Surface plasmon-enhanced fluorescence on Au nanohole array for prostate-specific antigen detection Zhang, Qingwen Wu, Lin Wong, Ten It Zhang, Jinling Liu, Xiaohu Zhou, Xiaodong Bai, Ping Liedberg, Bo Wang, Yi School of Materials Science & Engineering Centre for Biomimetic Sensor Science Gold nanohole array Localized surface plasmon Localized surface plasmon (LSP) has been widely applied for the enhancement of fluorescence emission for biosensing owing to its potential for strong field enhancement. However, due to its small penetration depth, LSP offers limited fluorescence enhancement over a whole sensor chip and, therefore, insufficient sensitivity for the detection of biomolecules, especially large molecules. We demonstrate the simultaneous excitation of LSP and propagating surface plasmon (PSP) on an Au nanohole array under Kretschmann configuration for the detection of prostate-specific antigen with a sandwich immunoassay. The proposed method combines the advantages of high field enhancement by LSP and large surface area probed by PSP field. The simulated results indicated that a maximum enhancement of electric field intensity up to 1,600 times can be achieved under the simultaneous excitation of LSP and PSP modes. The sandwich assay of PSA carried out on gold nanohole array substrate showed a limit of detection of 140 fM supporting coexcitation of LSP and PSP modes. The limit of detection was approximately sevenfold lower than that when only LSP was resonantly excited on the same substrate. The results of this study demonstrate high fluorescence enhancement through the coexcitation of LSP and PSP modes and pave a way for its implementation as a highly sensitive bioassay. ASTAR (Agency for Sci., Tech. and Research, S’pore) Published version 2017-08-16T04:20:33Z 2019-12-06T15:43:38Z 2017-08-16T04:20:33Z 2019-12-06T15:43:38Z 2017 Journal Article Zhang, Q., Wu, L., Wong, T. I., Zhang, J., Liu, X., Zhou, X., et al. (2017). Surface plasmon-enhanced fluorescence on Au nanohole array for prostate-specific antigen detection. International Journal of Nanomedicine, 12, 2307-2314. 1176-9114 https://hdl.handle.net/10356/84365 http://hdl.handle.net/10220/43589 10.2147/IJN.S128172 en International Journal of Nanomedicine © 2017 Zhang et al. This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms (https://www.dovepress.com/terms.php). 8 p. application/pdf
institution Nanyang Technological University
building NTU Library
continent Asia
country Singapore
Singapore
content_provider NTU Library
collection DR-NTU
language English
topic Gold nanohole array
Localized surface plasmon
spellingShingle Gold nanohole array
Localized surface plasmon
Zhang, Qingwen
Wu, Lin
Wong, Ten It
Zhang, Jinling
Liu, Xiaohu
Zhou, Xiaodong
Bai, Ping
Liedberg, Bo
Wang, Yi
Surface plasmon-enhanced fluorescence on Au nanohole array for prostate-specific antigen detection
description Localized surface plasmon (LSP) has been widely applied for the enhancement of fluorescence emission for biosensing owing to its potential for strong field enhancement. However, due to its small penetration depth, LSP offers limited fluorescence enhancement over a whole sensor chip and, therefore, insufficient sensitivity for the detection of biomolecules, especially large molecules. We demonstrate the simultaneous excitation of LSP and propagating surface plasmon (PSP) on an Au nanohole array under Kretschmann configuration for the detection of prostate-specific antigen with a sandwich immunoassay. The proposed method combines the advantages of high field enhancement by LSP and large surface area probed by PSP field. The simulated results indicated that a maximum enhancement of electric field intensity up to 1,600 times can be achieved under the simultaneous excitation of LSP and PSP modes. The sandwich assay of PSA carried out on gold nanohole array substrate showed a limit of detection of 140 fM supporting coexcitation of LSP and PSP modes. The limit of detection was approximately sevenfold lower than that when only LSP was resonantly excited on the same substrate. The results of this study demonstrate high fluorescence enhancement through the coexcitation of LSP and PSP modes and pave a way for its implementation as a highly sensitive bioassay.
author2 School of Materials Science & Engineering
author_facet School of Materials Science & Engineering
Zhang, Qingwen
Wu, Lin
Wong, Ten It
Zhang, Jinling
Liu, Xiaohu
Zhou, Xiaodong
Bai, Ping
Liedberg, Bo
Wang, Yi
format Article
author Zhang, Qingwen
Wu, Lin
Wong, Ten It
Zhang, Jinling
Liu, Xiaohu
Zhou, Xiaodong
Bai, Ping
Liedberg, Bo
Wang, Yi
author_sort Zhang, Qingwen
title Surface plasmon-enhanced fluorescence on Au nanohole array for prostate-specific antigen detection
title_short Surface plasmon-enhanced fluorescence on Au nanohole array for prostate-specific antigen detection
title_full Surface plasmon-enhanced fluorescence on Au nanohole array for prostate-specific antigen detection
title_fullStr Surface plasmon-enhanced fluorescence on Au nanohole array for prostate-specific antigen detection
title_full_unstemmed Surface plasmon-enhanced fluorescence on Au nanohole array for prostate-specific antigen detection
title_sort surface plasmon-enhanced fluorescence on au nanohole array for prostate-specific antigen detection
publishDate 2017
url https://hdl.handle.net/10356/84365
http://hdl.handle.net/10220/43589
_version_ 1772827532915113984