Metabolic engineering of terpene biosynthesis in plants using a trichome-specific transcription factor MsYABBY5 from spearmint (Mentha spicata)
In many aromatic plants including spearmint (Mentha spicata), the sites of secondary metabolite production are tiny specialized structures called peltate glandular trichomes (PGT). Having high commercial values, these secondary metabolites are exploited largely as flavours, fragrances and pharmaceut...
Saved in:
Main Authors: | , , , , , , , , |
---|---|
Other Authors: | |
Format: | Article |
Language: | English |
Published: |
2016
|
Subjects: | |
Online Access: | https://hdl.handle.net/10356/84535 http://hdl.handle.net/10220/41876 |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
Institution: | Nanyang Technological University |
Language: | English |
id |
sg-ntu-dr.10356-84535 |
---|---|
record_format |
dspace |
spelling |
sg-ntu-dr.10356-845352022-02-16T16:28:44Z Metabolic engineering of terpene biosynthesis in plants using a trichome-specific transcription factor MsYABBY5 from spearmint (Mentha spicata) Wang, Qian Reddy, Vaishnavi Amarr Panicker, Deepa Mao, Hui-Zhu Kumar, Nadimuthu Rajan, Chakravarthy Venkatesh, Prasanna Nori Chua, Nam-Hai Sarojam, Rajani Singapore Centre for Environmental Life Sciences Engineering spearmint secondary metabolism In many aromatic plants including spearmint (Mentha spicata), the sites of secondary metabolite production are tiny specialized structures called peltate glandular trichomes (PGT). Having high commercial values, these secondary metabolites are exploited largely as flavours, fragrances and pharmaceuticals. But, knowledge about transcription factors (TFs) that regulate secondary metabolism in PGT remains elusive. Understanding the role of TFs in secondary metabolism pathway will aid in metabolic engineering for increased yield of secondary metabolites and also the development of new production techniques for valuable metabolites. Here, we isolated and functionally characterized a novel MsYABBY5 gene that is preferentially expressed in PGT of spearmint. We generated transgenic plants in which MsYABBY5 was either overexpressed or silenced using RNA interference (RNAi). Analysis of the transgenic lines showed that the reduced expression of MsYABBY5 led to increased levels of terpenes and that overexpression decreased terpene levels. Additionally, ectopic expression of MsYABBY5 in Ocimum basilicum and Nicotiana sylvestris decreased secondary metabolite production in them, suggesting that the encoded transcription factor is probably a repressor of secondary metabolism. NRF (Natl Research Foundation, S’pore) Published version 2016-12-16T08:29:59Z 2019-12-06T15:46:44Z 2016-12-16T08:29:59Z 2019-12-06T15:46:44Z 2016 Journal Article Wang, Q., Reddy, V. A., Panicker, D., Mao, H.-Z., Kumar, N., Rajan, C., et al. (2016). Metabolic engineering of terpene biosynthesis in plants using a trichome-specific transcription factor MsYABBY5 from spearmint (Mentha spicata). Plant Biotechnology Journal, 14(7), 1619-1632. 1467-7644 https://hdl.handle.net/10356/84535 http://hdl.handle.net/10220/41876 10.1111/pbi.12525 26842602 en Plant Biotechnology Journal © 2016 The Authors. Plant Biotechnology Journal published by Society for Experimental Biology and The Association of Applied Biologists and John Wiley & Sons Ltd. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. 14 p. application/pdf |
institution |
Nanyang Technological University |
building |
NTU Library |
continent |
Asia |
country |
Singapore Singapore |
content_provider |
NTU Library |
collection |
DR-NTU |
language |
English |
topic |
spearmint secondary metabolism |
spellingShingle |
spearmint secondary metabolism Wang, Qian Reddy, Vaishnavi Amarr Panicker, Deepa Mao, Hui-Zhu Kumar, Nadimuthu Rajan, Chakravarthy Venkatesh, Prasanna Nori Chua, Nam-Hai Sarojam, Rajani Metabolic engineering of terpene biosynthesis in plants using a trichome-specific transcription factor MsYABBY5 from spearmint (Mentha spicata) |
description |
In many aromatic plants including spearmint (Mentha spicata), the sites of secondary metabolite production are tiny specialized structures called peltate glandular trichomes (PGT). Having high commercial values, these secondary metabolites are exploited largely as flavours, fragrances and pharmaceuticals. But, knowledge about transcription factors (TFs) that regulate secondary metabolism in PGT remains elusive. Understanding the role of TFs in secondary metabolism pathway will aid in metabolic engineering for increased yield of secondary metabolites and also the development of new production techniques for valuable metabolites. Here, we isolated and functionally characterized a novel MsYABBY5 gene that is preferentially expressed in PGT of spearmint. We generated transgenic plants in which MsYABBY5 was either overexpressed or silenced using RNA interference (RNAi). Analysis of the transgenic lines showed that the reduced expression of MsYABBY5 led to increased levels of terpenes and that overexpression decreased terpene levels. Additionally, ectopic expression of MsYABBY5 in Ocimum basilicum and Nicotiana sylvestris decreased secondary metabolite production in them, suggesting that the encoded transcription factor is probably a repressor of secondary metabolism. |
author2 |
Singapore Centre for Environmental Life Sciences Engineering |
author_facet |
Singapore Centre for Environmental Life Sciences Engineering Wang, Qian Reddy, Vaishnavi Amarr Panicker, Deepa Mao, Hui-Zhu Kumar, Nadimuthu Rajan, Chakravarthy Venkatesh, Prasanna Nori Chua, Nam-Hai Sarojam, Rajani |
format |
Article |
author |
Wang, Qian Reddy, Vaishnavi Amarr Panicker, Deepa Mao, Hui-Zhu Kumar, Nadimuthu Rajan, Chakravarthy Venkatesh, Prasanna Nori Chua, Nam-Hai Sarojam, Rajani |
author_sort |
Wang, Qian |
title |
Metabolic engineering of terpene biosynthesis in plants using a trichome-specific transcription factor MsYABBY5 from spearmint (Mentha spicata) |
title_short |
Metabolic engineering of terpene biosynthesis in plants using a trichome-specific transcription factor MsYABBY5 from spearmint (Mentha spicata) |
title_full |
Metabolic engineering of terpene biosynthesis in plants using a trichome-specific transcription factor MsYABBY5 from spearmint (Mentha spicata) |
title_fullStr |
Metabolic engineering of terpene biosynthesis in plants using a trichome-specific transcription factor MsYABBY5 from spearmint (Mentha spicata) |
title_full_unstemmed |
Metabolic engineering of terpene biosynthesis in plants using a trichome-specific transcription factor MsYABBY5 from spearmint (Mentha spicata) |
title_sort |
metabolic engineering of terpene biosynthesis in plants using a trichome-specific transcription factor msyabby5 from spearmint (mentha spicata) |
publishDate |
2016 |
url |
https://hdl.handle.net/10356/84535 http://hdl.handle.net/10220/41876 |
_version_ |
1725985699409166336 |