Microvalve bioprinting of cellular droplets with high resolution and consistency

• Main Authors: Ng, Wei Long, Yeong, Wai Yee, Naing, May Win
• Other Authors: School of Mechanical and Aerospace Engineering
• Format: Conference or Workshop Item
• Language: English
• Published: 2016
• Subjects: 3D printing; bioprinting
• Online Access: https://hdl.handle.net/10356/84558; http://hdl.handle.net/10220/41822
• Institution: Nanyang Technological University

Cellular printing via bioprinting approaches holds tremendous potential in fabrication of tissues/organs. One of the attractive traits of bioprinting systems include precise control over the spatial deposition of multiple types of cells. Notably, cell sedimentation during printing process poses a huge challenge and the ability to maintain printing consistency over time is required to realize potential scale-up of bioprinting processes. To achieve successful controlled cellular deposition, it is ideal to ensure an improved printing output consistency and high dispensing efficiency at high printing resolution over time. In this work, we demonstrated that the use of polyvinylpyrrolidone 360KDa (PVP360) polymer in printing solution enhances the printing resolution and output consistency over a period of 30 minutes. We also determined the minimum cellular density required to achieve a high dispensing efficiency of > 95%.