Nano-optofluidics for single molecule detection and sorting

• Main Authors: Yang, Y., Tsai, J. M., Liu, A. Q., Kwong, Dim Lee.
• Other Authors: School of Electrical and Electronic Engineering
• Format: Conference or Workshop Item
• Language: English
• Published: 2013
• Subjects: DRNTU::Engineering::Electrical and electronic engineering
• Online Access: https://hdl.handle.net/10356/84752; http://hdl.handle.net/10220/12467
• Institution: Nanyang Technological University

This paper reports a nano-optofluidic device using evanescent wave sensing for single molecule detection and sorting based on hydrodynamic focusing and total internal reflection (TIR). Nano-sized samples in liquid can be measured and counted even their size is smaller than the diffraction limit. We demonstrated 200-nm nanoparticle detecting which is beyond the diffraction limit. As compared to the conventional TIR microscopy, the nano-optofluidic device can measure and count all samples in real time. It has wide range applications in the single molecule detection, imaging and counting.