Glutamatergic axon-derived BDNF controls GABAergic synaptic differentiation in the cerebellum
To study mechanisms that regulate the construction of inhibitory circuits, we examined the role of brain-derived neurotrophic factor (BDNF) in the assembly of GABAergic inhibitory synapses in the mouse cerebellar cortex. We show that within the cerebellum, BDNF-expressing cells are restricted to the...
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sg-ntu-dr.10356-849052023-02-28T17:05:06Z Glutamatergic axon-derived BDNF controls GABAergic synaptic differentiation in the cerebellum Zang, Keling Masliah, Eliezer Chen, Albert I-Ming Reichardt, Louis French School of Biological Sciences Warwick-NTU Neuroscience Programme GABAergic Neurons DRNTU::Science::Biological sciences Brain-Derived Neurotrophic Factor To study mechanisms that regulate the construction of inhibitory circuits, we examined the role of brain-derived neurotrophic factor (BDNF) in the assembly of GABAergic inhibitory synapses in the mouse cerebellar cortex. We show that within the cerebellum, BDNF-expressing cells are restricted to the internal granular layer (IGL), but that the BDNF protein is present within mossy fibers which originate from cells located outside of the cerebellum. In contrast to deletion of TrkB, the cognate receptor for BDNF, deletion of Bdnf from cerebellar cell bodies alone did not perturb the localization of pre- or postsynaptic constituents at the GABAergic synapses formed by Golgi cell axons on granule cell dendrites within the IGL. Instead, we found that BDNF derived from excitatory mossy fiber endings controls their differentiation. Our findings thus indicate that cerebellar BDNF is derived primarily from excitatory neurons—precerebellar nuclei/spinal cord neurons that give rise to mossy fibers—and promotes GABAergic synapse formation as a result of release from axons. Thus, within the cerebellum the preferential localization of BDNF to axons enhances the specificity through which BDNF promotes GABAergic synaptic differentiation. MOE (Min. of Education, S’pore) Published version 2018-11-21T04:51:58Z 2019-12-06T15:53:25Z 2018-11-21T04:51:58Z 2019-12-06T15:53:25Z 2016 Journal Article Chen, A. I.-M, Zang, K., Masliah, E., & Reichardt, L. F. (2016). Glutamatergic axon-derived BDNF controls GABAergic synaptic differentiation in the cerebellum. Scientific Reports, 6, 20201-. doi:10.1038/srep20201 https://hdl.handle.net/10356/84905 http://hdl.handle.net/10220/46670 10.1038/srep20201 26830657 en Scientific Reports © 2016 The Authors (Nature Publishing Group). This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ 13 p. application/pdf |
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GABAergic Neurons DRNTU::Science::Biological sciences Brain-Derived Neurotrophic Factor Zang, Keling Masliah, Eliezer Chen, Albert I-Ming Reichardt, Louis French Glutamatergic axon-derived BDNF controls GABAergic synaptic differentiation in the cerebellum |
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To study mechanisms that regulate the construction of inhibitory circuits, we examined the role of brain-derived neurotrophic factor (BDNF) in the assembly of GABAergic inhibitory synapses in the mouse cerebellar cortex. We show that within the cerebellum, BDNF-expressing cells are restricted to the internal granular layer (IGL), but that the BDNF protein is present within mossy fibers which originate from cells located outside of the cerebellum. In contrast to deletion of TrkB, the cognate receptor for BDNF, deletion of Bdnf from cerebellar cell bodies alone did not perturb the localization of pre- or postsynaptic constituents at the GABAergic synapses formed by Golgi cell axons on granule cell dendrites within the IGL. Instead, we found that BDNF derived from excitatory mossy fiber endings controls their differentiation. Our findings thus indicate that cerebellar BDNF is derived primarily from excitatory neurons—precerebellar nuclei/spinal cord neurons that give rise to mossy fibers—and promotes GABAergic synapse formation as a result of release from axons. Thus, within the cerebellum the preferential localization of BDNF to axons enhances the specificity through which BDNF promotes GABAergic synaptic differentiation. |
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School of Biological Sciences |
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School of Biological Sciences Zang, Keling Masliah, Eliezer Chen, Albert I-Ming Reichardt, Louis French |
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Article |
author |
Zang, Keling Masliah, Eliezer Chen, Albert I-Ming Reichardt, Louis French |
author_sort |
Zang, Keling |
title |
Glutamatergic axon-derived BDNF controls GABAergic synaptic differentiation in the cerebellum |
title_short |
Glutamatergic axon-derived BDNF controls GABAergic synaptic differentiation in the cerebellum |
title_full |
Glutamatergic axon-derived BDNF controls GABAergic synaptic differentiation in the cerebellum |
title_fullStr |
Glutamatergic axon-derived BDNF controls GABAergic synaptic differentiation in the cerebellum |
title_full_unstemmed |
Glutamatergic axon-derived BDNF controls GABAergic synaptic differentiation in the cerebellum |
title_sort |
glutamatergic axon-derived bdnf controls gabaergic synaptic differentiation in the cerebellum |
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2018 |
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https://hdl.handle.net/10356/84905 http://hdl.handle.net/10220/46670 |
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1759854234182877184 |