Quantum changes in Helicobacter pylori gene expression accompany host-adaptation

Helicobacter pylori is a highly successful gastric pathogen. High genomic plasticity allows its adaptation to changing host environments. Complete genomes of H. pylori clinical isolate UM032 and its mice-adapted serial derivatives 298 and 299, generated using both PacBio RS and Illumina MiSeq sequen...

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Main Authors: Chua, Eng-Guan, Wise, Michael J., Khosravi, Yalda, Seow, Shih-Wee, Amoyo, Arlaine A., Pettersson, Sven, Peters, Fanny, Tay, Chin-Yen, Perkins, Timothy T., Loke, Mun-Fai, Marshall, Barry J., Vadivelu, Jamuna
Other Authors: Lee Kong Chian School of Medicine (LKCMedicine)
Format: Article
Language:English
Published: 2017
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Online Access:https://hdl.handle.net/10356/85100
http://hdl.handle.net/10220/43657
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Institution: Nanyang Technological University
Language: English
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spelling sg-ntu-dr.10356-851002020-11-01T05:21:38Z Quantum changes in Helicobacter pylori gene expression accompany host-adaptation Chua, Eng-Guan Wise, Michael J. Khosravi, Yalda Seow, Shih-Wee Amoyo, Arlaine A. Pettersson, Sven Peters, Fanny Tay, Chin-Yen Perkins, Timothy T. Loke, Mun-Fai Marshall, Barry J. Vadivelu, Jamuna Lee Kong Chian School of Medicine (LKCMedicine) Singapore Centre for Environmental Life Sciences and Engineering BabA Helicobacter pylori Helicobacter pylori is a highly successful gastric pathogen. High genomic plasticity allows its adaptation to changing host environments. Complete genomes of H. pylori clinical isolate UM032 and its mice-adapted serial derivatives 298 and 299, generated using both PacBio RS and Illumina MiSeq sequencing technologies, were compared to identify novel elements responsible for host-adaptation. The acquisition of a jhp0562-like allele, which encodes for a galactosyltransferase, was identified in the mice-adapted strains. Our analysis implies a new β-1,4-galactosyltransferase role for this enzyme, essential for Ley antigen expression. Intragenomic recombination between babA and babB genes was also observed. Further, we expanded on the list of candidate genes whose expression patterns have been mediated by upstream homopolymer-length alterations to facilitate host adaption. Importantly, greater than four-fold reduction of mRNA levels was demonstrated in five genes. Among the down-regulated genes, three encode for outer membrane proteins, including BabA, BabB and HopD. As expected, a substantial reduction in BabA protein abundance was detected in mice-adapted strains 298 and 299 via Western analysis. Our results suggest that the expression of Ley antigen and reduced outer membrane protein expressions may facilitate H. pylori colonisation of mouse gastric epithelium. Published version 2017-08-31T02:04:46Z 2019-12-06T15:57:03Z 2017-08-31T02:04:46Z 2019-12-06T15:57:03Z 2016 Journal Article Chua, E.-G., Wise, M. J., Khosravi, Y., Seow, S.-W., Amoyo, A. A., Pettersson, S., et al. (2017). Quantum changes in Helicobacter pylori gene expression accompany host-adaptation. DNA Research, 24(1), 37-49. 1340-2838 https://hdl.handle.net/10356/85100 http://hdl.handle.net/10220/43657 10.1093/dnares/dsw046 en DNA Research © 2016 The Author. Published by Oxford University Press on behalf of Kazusa DNA Research Institute. This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contactjournals.permissions@oup.com 13 p. application/pdf
institution Nanyang Technological University
building NTU Library
continent Asia
country Singapore
Singapore
content_provider NTU Library
collection DR-NTU
language English
topic BabA
Helicobacter pylori
spellingShingle BabA
Helicobacter pylori
Chua, Eng-Guan
Wise, Michael J.
Khosravi, Yalda
Seow, Shih-Wee
Amoyo, Arlaine A.
Pettersson, Sven
Peters, Fanny
Tay, Chin-Yen
Perkins, Timothy T.
Loke, Mun-Fai
Marshall, Barry J.
Vadivelu, Jamuna
Quantum changes in Helicobacter pylori gene expression accompany host-adaptation
description Helicobacter pylori is a highly successful gastric pathogen. High genomic plasticity allows its adaptation to changing host environments. Complete genomes of H. pylori clinical isolate UM032 and its mice-adapted serial derivatives 298 and 299, generated using both PacBio RS and Illumina MiSeq sequencing technologies, were compared to identify novel elements responsible for host-adaptation. The acquisition of a jhp0562-like allele, which encodes for a galactosyltransferase, was identified in the mice-adapted strains. Our analysis implies a new β-1,4-galactosyltransferase role for this enzyme, essential for Ley antigen expression. Intragenomic recombination between babA and babB genes was also observed. Further, we expanded on the list of candidate genes whose expression patterns have been mediated by upstream homopolymer-length alterations to facilitate host adaption. Importantly, greater than four-fold reduction of mRNA levels was demonstrated in five genes. Among the down-regulated genes, three encode for outer membrane proteins, including BabA, BabB and HopD. As expected, a substantial reduction in BabA protein abundance was detected in mice-adapted strains 298 and 299 via Western analysis. Our results suggest that the expression of Ley antigen and reduced outer membrane protein expressions may facilitate H. pylori colonisation of mouse gastric epithelium.
author2 Lee Kong Chian School of Medicine (LKCMedicine)
author_facet Lee Kong Chian School of Medicine (LKCMedicine)
Chua, Eng-Guan
Wise, Michael J.
Khosravi, Yalda
Seow, Shih-Wee
Amoyo, Arlaine A.
Pettersson, Sven
Peters, Fanny
Tay, Chin-Yen
Perkins, Timothy T.
Loke, Mun-Fai
Marshall, Barry J.
Vadivelu, Jamuna
format Article
author Chua, Eng-Guan
Wise, Michael J.
Khosravi, Yalda
Seow, Shih-Wee
Amoyo, Arlaine A.
Pettersson, Sven
Peters, Fanny
Tay, Chin-Yen
Perkins, Timothy T.
Loke, Mun-Fai
Marshall, Barry J.
Vadivelu, Jamuna
author_sort Chua, Eng-Guan
title Quantum changes in Helicobacter pylori gene expression accompany host-adaptation
title_short Quantum changes in Helicobacter pylori gene expression accompany host-adaptation
title_full Quantum changes in Helicobacter pylori gene expression accompany host-adaptation
title_fullStr Quantum changes in Helicobacter pylori gene expression accompany host-adaptation
title_full_unstemmed Quantum changes in Helicobacter pylori gene expression accompany host-adaptation
title_sort quantum changes in helicobacter pylori gene expression accompany host-adaptation
publishDate 2017
url https://hdl.handle.net/10356/85100
http://hdl.handle.net/10220/43657
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