Capsid integrity qPCR—an Azo-dye based and culture-independent approach to estimate adenovirus infectivity after disinfection and in the aquatic environment

• Main Authors: Leifels, Mats, Shoults, David, Wiedemeyer, Alyssa, Ashbolt, Nicholas J., Sozzi, Emanuele, Hagemeier, Angela, Jurzik, Lars
• Other Authors: Singapore Centre for Environmental Life Sciences and Engineering
• Format: Article
• Language: English
• Published: 2019
• Subjects: Engineering::Environmental engineering::Water treatment; Science::Biological sciences::Molecular biology; Capsid Integrity qPCR; Human Adenovirus
• Online Access: https://hdl.handle.net/10356/85313; http://hdl.handle.net/10220/49800
• Institution: Nanyang Technological University

Recreational, reclaimed and drinking source waters worldwide are under increasing anthropogenic pressure, and often contain waterborne enteric bacterial, protozoan, and viral pathogens originating from non-point source fecal contamination. Recently, the capsid integrity (ci)-qPCR, utilizing the azo-...