Formation of cell spheroids using Standing Surface Acoustic Wave (SSAW)
3D bioprinting becomes one of the popular approaches in the tissue engineering. In this emerging application, bioink is crucial for fabrication and functionality of constructed tissue. The use of cell spheroids as bioink can enhance the cell-cell interaction and subsequently the growth and different...
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sg-ntu-dr.10356-853332023-03-04T17:15:06Z Formation of cell spheroids using Standing Surface Acoustic Wave (SSAW) Sriphutkiat, Yannapol Kasetsirikul, Surasak Zhou, Yufeng School of Mechanical and Aerospace Engineering Singapore Centre for 3D Printing Cell Spheroid Standing Surface Acoustic Wave (SSAW) 3D bioprinting becomes one of the popular approaches in the tissue engineering. In this emerging application, bioink is crucial for fabrication and functionality of constructed tissue. The use of cell spheroids as bioink can enhance the cell-cell interaction and subsequently the growth and differentiation of cells in the 3D printed construct with the minimal amount of other biomaterials. However, the conventional methods of preparing the cell spheroids have several limitations, such as long culture time, low-throughput, and medium modification. In this study, the formation of cell spheroids by SSAW was evaluated both numerically and experimentally in order to overcome the aforementioned limitations. The effects of excitation frequencies on the cell accumulation time, diameter of formed cell spheroids, and subsequently, the growth and viability of cell spheroids in the culture media over time were studied. Using the high-frequency (24.9 MHz) excitation, cell accumulation time to the pressure nodes could be reduced in comparison to that of the low-frequency (10.4 MHz) excitation, but in a smaller spheroid size. SSAW excitation at both frequencies does not affect the cell viabilities up to 7 days, > 90% with no statistical difference compared with the control group. In summary, SSAW can effectively prepare the cell spheroids as bioink for the future 3D bioprinting and various biotechnology applications (e.g., pharmaceutical drug screening and tissue engineering). ASTAR (Agency for Sci., Tech. and Research, S’pore) MOE (Min. of Education, S’pore) Published version 2018-07-20T02:37:21Z 2019-12-06T16:01:48Z 2018-07-20T02:37:21Z 2019-12-06T16:01:48Z 2018 Journal Article Sriphutkiat, Y., Kasetsirikul, S., & Zhou, Y. (2017). Formation of cell spheroids using Standing Surface Acoustic Wave (SSAW). International Journal of Bioprinting, 4(1), 130-. https://hdl.handle.net/10356/85333 http://hdl.handle.net/10220/45148 10.18063/ijb.v4i1.130 en International Journal of Bioprinting © 2018 The Author(s) (International Journal of Bioprinting) (published by Whioce Publishing Pte Ltd). This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/), permitting all non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. 12 p. application/pdf |
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Cell Spheroid Standing Surface Acoustic Wave (SSAW) Sriphutkiat, Yannapol Kasetsirikul, Surasak Zhou, Yufeng Formation of cell spheroids using Standing Surface Acoustic Wave (SSAW) |
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3D bioprinting becomes one of the popular approaches in the tissue engineering. In this emerging application, bioink is crucial for fabrication and functionality of constructed tissue. The use of cell spheroids as bioink can enhance the cell-cell interaction and subsequently the growth and differentiation of cells in the 3D printed construct with the minimal amount of other biomaterials. However, the conventional methods of preparing the cell spheroids have several limitations, such as long culture time, low-throughput, and medium modification. In this study, the formation of cell spheroids by SSAW was evaluated both numerically and experimentally in order to overcome the aforementioned limitations. The effects of excitation frequencies on the cell accumulation time, diameter of formed cell spheroids, and subsequently, the growth and viability of cell spheroids in the culture media over time were studied. Using the high-frequency (24.9 MHz) excitation, cell accumulation time to the pressure nodes could be reduced in comparison to that of the low-frequency (10.4 MHz) excitation, but in a smaller spheroid size. SSAW excitation at both frequencies does not affect the cell viabilities up to 7 days, > 90% with no statistical difference compared with the control group. In summary, SSAW can effectively prepare the cell spheroids as bioink for the future 3D bioprinting and various biotechnology applications (e.g., pharmaceutical drug screening and tissue engineering). |
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School of Mechanical and Aerospace Engineering |
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School of Mechanical and Aerospace Engineering Sriphutkiat, Yannapol Kasetsirikul, Surasak Zhou, Yufeng |
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Article |
author |
Sriphutkiat, Yannapol Kasetsirikul, Surasak Zhou, Yufeng |
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Sriphutkiat, Yannapol |
title |
Formation of cell spheroids using Standing Surface Acoustic Wave (SSAW) |
title_short |
Formation of cell spheroids using Standing Surface Acoustic Wave (SSAW) |
title_full |
Formation of cell spheroids using Standing Surface Acoustic Wave (SSAW) |
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Formation of cell spheroids using Standing Surface Acoustic Wave (SSAW) |
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Formation of cell spheroids using Standing Surface Acoustic Wave (SSAW) |
title_sort |
formation of cell spheroids using standing surface acoustic wave (ssaw) |
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2018 |
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https://hdl.handle.net/10356/85333 http://hdl.handle.net/10220/45148 |
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