Characterization of a novel multidrug resistance plasmid pSGB23 isolated from Salmonella enterica subspecies enterica serovar Saintpaul

Background: Salmonella enterica subspecies enterica serovar Saintpaul (S. Saintpaul) is an important gut pathogen which causes salmonellosis worldwide. Although intestinal salmonellosis is usually self-limiting, it can be life-threatening in children, the elderlies and immunocompromised patients. Ap...

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Main Authors: Drautz-Moses, Daniela Isabel, Ding, Yichen, Zwe, Ye Htut, Chin, Seow Fong, Givskov, Michael, Schlundt, Jorgen, Yuk, Hyun-Gyun, Yang, Liang, Kohli, Gurjeet Singh, Schuster, Stephan Christoph
Other Authors: School of Chemical and Biomedical Engineering
Format: Article
Language:English
Published: 2018
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Online Access:https://hdl.handle.net/10356/86184
http://hdl.handle.net/10220/45250
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Institution: Nanyang Technological University
Language: English
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spelling sg-ntu-dr.10356-861842020-09-21T11:34:15Z Characterization of a novel multidrug resistance plasmid pSGB23 isolated from Salmonella enterica subspecies enterica serovar Saintpaul Drautz-Moses, Daniela Isabel Ding, Yichen Zwe, Ye Htut Chin, Seow Fong Givskov, Michael Schlundt, Jorgen Yuk, Hyun-Gyun Yang, Liang Kohli, Gurjeet Singh Schuster, Stephan Christoph School of Chemical and Biomedical Engineering School of Biological Sciences Interdisciplinary Graduate School (IGS) Singapore Centre for Environmental Life Sciences Engineering NTU Food Technology Centre Multidrug Resistance Plasmid Salmonella Background: Salmonella enterica subspecies enterica serovar Saintpaul (S. Saintpaul) is an important gut pathogen which causes salmonellosis worldwide. Although intestinal salmonellosis is usually self-limiting, it can be life-threatening in children, the elderlies and immunocompromised patients. Appropriate antibiotic treatment is therefore required for these patients. However, the efficacy of many antibiotics on S. enterica infections has been greatly compromised due to spreading of multidrug resistance (MDR) plasmids, which poses serious threats on public health and needs to be closely monitored. In this study, we sequenced and fully characterized an S. enterica MDR plasmid pSGB23 isolated from chicken. Results: Complete genome sequence analysis revealed that S. Saintpaul strain SGB23 harbored a 254 kb megaplasmid pSGB23, which carries 11 antibiotic resistance genes responsible for resistance to 9 classes of antibiotics and quaternary ammonium compounds that are commonly used to disinfect food processing facilities. Furthermore, we found that pSGB23 carries multiple conjugative systems, which allow it to spread into other Enterobacteriaceae spp. by self-conjugation. It also harbors multiple types of replicons and plasmid maintenance and addictive systems, which explains its broad host range and stable inheritance. Conclusions: We report here a novel MDR plasmid pSGB23 harboured by S. enterica. To our knowledge, it carried the greatest number of antibiotic resistance genes with the broadest range of resistance spectrum among S. enterica MDR plasmids identified so far. The isolation of pSGB23 from food sources is worrisome, while surveillance on its further spreading will be carried out based on the findings reported in this study. NRF (Natl Research Foundation, S’pore) MOE (Min. of Education, S’pore) Published version 2018-07-26T05:10:23Z 2019-12-06T16:17:32Z 2018-07-26T05:10:23Z 2019-12-06T16:17:32Z 2018 Journal Article Ding, Y., Zwe, Y. H., Chin, S. F., Kohli, G. S., Drautz-Moses, D. I., Givskov, M., et al. (2018). Characterization of a novel multidrug resistance plasmid pSGB23 isolated from Salmonella enterica subspecies enterica serovar Saintpaul. Gut Pathogens, 10(1), 20-. https://hdl.handle.net/10356/86184 http://hdl.handle.net/10220/45250 10.1186/s13099-018-0249-6 en Gut Pathogens © 2018 The Author(s). This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. 5 p. application/pdf
institution Nanyang Technological University
building NTU Library
country Singapore
collection DR-NTU
language English
topic Multidrug Resistance Plasmid
Salmonella
spellingShingle Multidrug Resistance Plasmid
Salmonella
Drautz-Moses, Daniela Isabel
Ding, Yichen
Zwe, Ye Htut
Chin, Seow Fong
Givskov, Michael
Schlundt, Jorgen
Yuk, Hyun-Gyun
Yang, Liang
Kohli, Gurjeet Singh
Schuster, Stephan Christoph
Characterization of a novel multidrug resistance plasmid pSGB23 isolated from Salmonella enterica subspecies enterica serovar Saintpaul
description Background: Salmonella enterica subspecies enterica serovar Saintpaul (S. Saintpaul) is an important gut pathogen which causes salmonellosis worldwide. Although intestinal salmonellosis is usually self-limiting, it can be life-threatening in children, the elderlies and immunocompromised patients. Appropriate antibiotic treatment is therefore required for these patients. However, the efficacy of many antibiotics on S. enterica infections has been greatly compromised due to spreading of multidrug resistance (MDR) plasmids, which poses serious threats on public health and needs to be closely monitored. In this study, we sequenced and fully characterized an S. enterica MDR plasmid pSGB23 isolated from chicken. Results: Complete genome sequence analysis revealed that S. Saintpaul strain SGB23 harbored a 254 kb megaplasmid pSGB23, which carries 11 antibiotic resistance genes responsible for resistance to 9 classes of antibiotics and quaternary ammonium compounds that are commonly used to disinfect food processing facilities. Furthermore, we found that pSGB23 carries multiple conjugative systems, which allow it to spread into other Enterobacteriaceae spp. by self-conjugation. It also harbors multiple types of replicons and plasmid maintenance and addictive systems, which explains its broad host range and stable inheritance. Conclusions: We report here a novel MDR plasmid pSGB23 harboured by S. enterica. To our knowledge, it carried the greatest number of antibiotic resistance genes with the broadest range of resistance spectrum among S. enterica MDR plasmids identified so far. The isolation of pSGB23 from food sources is worrisome, while surveillance on its further spreading will be carried out based on the findings reported in this study.
author2 School of Chemical and Biomedical Engineering
author_facet School of Chemical and Biomedical Engineering
Drautz-Moses, Daniela Isabel
Ding, Yichen
Zwe, Ye Htut
Chin, Seow Fong
Givskov, Michael
Schlundt, Jorgen
Yuk, Hyun-Gyun
Yang, Liang
Kohli, Gurjeet Singh
Schuster, Stephan Christoph
format Article
author Drautz-Moses, Daniela Isabel
Ding, Yichen
Zwe, Ye Htut
Chin, Seow Fong
Givskov, Michael
Schlundt, Jorgen
Yuk, Hyun-Gyun
Yang, Liang
Kohli, Gurjeet Singh
Schuster, Stephan Christoph
author_sort Drautz-Moses, Daniela Isabel
title Characterization of a novel multidrug resistance plasmid pSGB23 isolated from Salmonella enterica subspecies enterica serovar Saintpaul
title_short Characterization of a novel multidrug resistance plasmid pSGB23 isolated from Salmonella enterica subspecies enterica serovar Saintpaul
title_full Characterization of a novel multidrug resistance plasmid pSGB23 isolated from Salmonella enterica subspecies enterica serovar Saintpaul
title_fullStr Characterization of a novel multidrug resistance plasmid pSGB23 isolated from Salmonella enterica subspecies enterica serovar Saintpaul
title_full_unstemmed Characterization of a novel multidrug resistance plasmid pSGB23 isolated from Salmonella enterica subspecies enterica serovar Saintpaul
title_sort characterization of a novel multidrug resistance plasmid psgb23 isolated from salmonella enterica subspecies enterica serovar saintpaul
publishDate 2018
url https://hdl.handle.net/10356/86184
http://hdl.handle.net/10220/45250
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