Electrochemical detection of uric acid on exfoliated nanosheets of graphitic-like carbon nitride (g-C3N4) based sensor

A highly sensitive, selective and stable electrochemical sensor for detection of uric acid (UA) in aqueous solution has been successfully developed by deposition of exfoliated graphitic-like carbon nitride (g-C3N4) nanosheets on glassy carbon electrode (GCE). The synthesized g-C3N4 was confirmed by...

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Main Authors: Chan-Park, Mary Bee Eng, Murugan, N., Sundramoorthy, Ashok K.
Other Authors: School of Chemical and Biomedical Engineering
Format: Article
Language:English
Published: 2019
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Online Access:https://hdl.handle.net/10356/86225
http://hdl.handle.net/10220/49258
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spelling sg-ntu-dr.10356-862252023-12-29T06:45:13Z Electrochemical detection of uric acid on exfoliated nanosheets of graphitic-like carbon nitride (g-C3N4) based sensor Chan-Park, Mary Bee Eng Murugan, N. Sundramoorthy, Ashok K. School of Chemical and Biomedical Engineering Electrochemical sensors Sensors Engineering::Electrical and electronic engineering A highly sensitive, selective and stable electrochemical sensor for detection of uric acid (UA) in aqueous solution has been successfully developed by deposition of exfoliated graphitic-like carbon nitride (g-C3N4) nanosheets on glassy carbon electrode (GCE). The synthesized g-C3N4 was confirmed by X-ray diffraction (XRD), Fourier-transform infrared (FT-IR) and Raman spectroscopies. Field-emission scanning electron microscopy (FE-SEM) and High-resolution transmission electron microscopy (HR-TEM) were used to investigate the crystalline structure of g-C3N4. The elemental composition was characterized by energy-dispersive X-ray spectroscopy (EDXS). Compared to bare GCE, exfoliated g-C3N4 nanosheets (NS) modified GCE exhibited higher catalytic current for UA electro-oxidation at reduced over potential in 0.1 M phosphate buffered saline solution (PBS), which is essential to discriminate interfering analytes. g-C3N4 NS modified GCE showed a linear relationship between the electrochemical signal and the UA concentration from 100 to 1000 μM with fast response by differential pulse voltammetry (DPV). The common interferent molecules such as dopamine, ascorbic acid, folic acid, paracetamol, lactic acid, oxalic acid, cysteine, and ciprofloxacin were tested in 0.1 M PBS for the g-C3N4 NS modified GCE. It was found that these molecules did not affect the oxidation current of UA when they co-existed in the same buffer solution. Moreover, the modified sensor probe was tested for UA in urine samples with satisfactory recovery values. The proposed sensor offers high accuracy, sensitivity, simple fabrication and low cost. We suggest that g-C3N4 NS based sensor can be useful for UA analysis in medical, environmental, food and industrial applications. Published version 2019-07-10T07:42:27Z 2019-12-06T16:18:26Z 2019-07-10T07:42:27Z 2019-12-06T16:18:26Z 2019 Journal Article Murugan, N., Chan-Park, M. B., & Sundramoorthy, A. K. (2019). Electrochemical detection of uric acid on exfoliated nanosheets of graphitic-like carbon nitride (g-C3N4) based sensor. Journal of The Electrochemical Society, 166(9), B3163-B3170. doi:10.1149/2.0261909jes 0013-4651 https://hdl.handle.net/10356/86225 http://hdl.handle.net/10220/49258 10.1149/2.0261909jes en Journal of The Electrochemical Society © 2019 The Author(s). Published by ECS. This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 License (CC BY, http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse of the work in any medium, provided the original work is properly cited. [DOI: 10.1149/2.0261909jes] 8 p. application/pdf
institution Nanyang Technological University
building NTU Library
continent Asia
country Singapore
Singapore
content_provider NTU Library
collection DR-NTU
language English
topic Electrochemical sensors
Sensors
Engineering::Electrical and electronic engineering
spellingShingle Electrochemical sensors
Sensors
Engineering::Electrical and electronic engineering
Chan-Park, Mary Bee Eng
Murugan, N.
Sundramoorthy, Ashok K.
Electrochemical detection of uric acid on exfoliated nanosheets of graphitic-like carbon nitride (g-C3N4) based sensor
description A highly sensitive, selective and stable electrochemical sensor for detection of uric acid (UA) in aqueous solution has been successfully developed by deposition of exfoliated graphitic-like carbon nitride (g-C3N4) nanosheets on glassy carbon electrode (GCE). The synthesized g-C3N4 was confirmed by X-ray diffraction (XRD), Fourier-transform infrared (FT-IR) and Raman spectroscopies. Field-emission scanning electron microscopy (FE-SEM) and High-resolution transmission electron microscopy (HR-TEM) were used to investigate the crystalline structure of g-C3N4. The elemental composition was characterized by energy-dispersive X-ray spectroscopy (EDXS). Compared to bare GCE, exfoliated g-C3N4 nanosheets (NS) modified GCE exhibited higher catalytic current for UA electro-oxidation at reduced over potential in 0.1 M phosphate buffered saline solution (PBS), which is essential to discriminate interfering analytes. g-C3N4 NS modified GCE showed a linear relationship between the electrochemical signal and the UA concentration from 100 to 1000 μM with fast response by differential pulse voltammetry (DPV). The common interferent molecules such as dopamine, ascorbic acid, folic acid, paracetamol, lactic acid, oxalic acid, cysteine, and ciprofloxacin were tested in 0.1 M PBS for the g-C3N4 NS modified GCE. It was found that these molecules did not affect the oxidation current of UA when they co-existed in the same buffer solution. Moreover, the modified sensor probe was tested for UA in urine samples with satisfactory recovery values. The proposed sensor offers high accuracy, sensitivity, simple fabrication and low cost. We suggest that g-C3N4 NS based sensor can be useful for UA analysis in medical, environmental, food and industrial applications.
author2 School of Chemical and Biomedical Engineering
author_facet School of Chemical and Biomedical Engineering
Chan-Park, Mary Bee Eng
Murugan, N.
Sundramoorthy, Ashok K.
format Article
author Chan-Park, Mary Bee Eng
Murugan, N.
Sundramoorthy, Ashok K.
author_sort Chan-Park, Mary Bee Eng
title Electrochemical detection of uric acid on exfoliated nanosheets of graphitic-like carbon nitride (g-C3N4) based sensor
title_short Electrochemical detection of uric acid on exfoliated nanosheets of graphitic-like carbon nitride (g-C3N4) based sensor
title_full Electrochemical detection of uric acid on exfoliated nanosheets of graphitic-like carbon nitride (g-C3N4) based sensor
title_fullStr Electrochemical detection of uric acid on exfoliated nanosheets of graphitic-like carbon nitride (g-C3N4) based sensor
title_full_unstemmed Electrochemical detection of uric acid on exfoliated nanosheets of graphitic-like carbon nitride (g-C3N4) based sensor
title_sort electrochemical detection of uric acid on exfoliated nanosheets of graphitic-like carbon nitride (g-c3n4) based sensor
publishDate 2019
url https://hdl.handle.net/10356/86225
http://hdl.handle.net/10220/49258
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