Rupturing cancer cells by the expansion of functionalized stimuli-responsive hydrogels

Using particles with different functionalities for treating cancer has many advantages over other methods (for example, better access to remote parts of the body); however, current chemical (for example, chemotherapy) and biological (for example, immunotherapy) methods still face many challenges. He...

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Main Authors: Fang, Yan, Tan, Jiajun, Lim, Sierin, Soh, Siowling
Other Authors: School of Chemical and Biomedical Engineering
Format: Article
Language:English
Published: 2018
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Online Access:https://hdl.handle.net/10356/86288
http://hdl.handle.net/10220/45241
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Institution: Nanyang Technological University
Language: English
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spelling sg-ntu-dr.10356-862882023-12-29T06:53:20Z Rupturing cancer cells by the expansion of functionalized stimuli-responsive hydrogels Fang, Yan Tan, Jiajun Lim, Sierin Soh, Siowling School of Chemical and Biomedical Engineering Hydrogels Cancer Cells Using particles with different functionalities for treating cancer has many advantages over other methods (for example, better access to remote parts of the body); however, current chemical (for example, chemotherapy) and biological (for example, immunotherapy) methods still face many challenges. Here, we describe a fundamentally different approach: using the physical force of an expanding stimuli-responsive hydrogel to rupture cancer cells attached on its surface. Specifically, we coated temperature-responsive hydrogels with a layer of cell-adherent arginine-glycine-aspartate (RGD) peptides. The approach involved first allowing cancer cells to attach onto the surface of the hydrogels, and then applying a change in temperature. As the hydrogel underwent a chemical transformation and expanded due to the stimulus, the cancer cells attached to it ruptured. The results from staining the cells with trypan blue, observing them using SEM, and analyzing them using the MTT assay showed that both breast and lung cancer cells died after the hydrogel expanded; hence, we showed that this physical force from the expanding hydrogel is strong enough to rupture the cancer cells. In addition, the force derived from the expanding hydrogel was determined separately to be larger than that needed to rupture typical cells. This physical approach is conceptually simple, technically easy to implement, and potentially generalizable for rupturing a wide range of cells. MOE (Min. of Education, S’pore) Published version 2018-07-26T01:50:28Z 2019-12-06T16:19:41Z 2018-07-26T01:50:28Z 2019-12-06T16:19:41Z 2018 Journal Article Fang, Y., Tan, J., Lim, S., & Soh, S. (2018). Rupturing cancer cells by the expansion of functionalized stimuli-responsive hydrogels. NPG Asia Materials, 10(2), e465-. https://hdl.handle.net/10356/86288 http://hdl.handle.net/10220/45241 10.1038/am.2017.232 en NPG Asia Materials © 2018 The Author(s). This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ 9 p. application/pdf
institution Nanyang Technological University
building NTU Library
continent Asia
country Singapore
Singapore
content_provider NTU Library
collection DR-NTU
language English
topic Hydrogels
Cancer Cells
spellingShingle Hydrogels
Cancer Cells
Fang, Yan
Tan, Jiajun
Lim, Sierin
Soh, Siowling
Rupturing cancer cells by the expansion of functionalized stimuli-responsive hydrogels
description Using particles with different functionalities for treating cancer has many advantages over other methods (for example, better access to remote parts of the body); however, current chemical (for example, chemotherapy) and biological (for example, immunotherapy) methods still face many challenges. Here, we describe a fundamentally different approach: using the physical force of an expanding stimuli-responsive hydrogel to rupture cancer cells attached on its surface. Specifically, we coated temperature-responsive hydrogels with a layer of cell-adherent arginine-glycine-aspartate (RGD) peptides. The approach involved first allowing cancer cells to attach onto the surface of the hydrogels, and then applying a change in temperature. As the hydrogel underwent a chemical transformation and expanded due to the stimulus, the cancer cells attached to it ruptured. The results from staining the cells with trypan blue, observing them using SEM, and analyzing them using the MTT assay showed that both breast and lung cancer cells died after the hydrogel expanded; hence, we showed that this physical force from the expanding hydrogel is strong enough to rupture the cancer cells. In addition, the force derived from the expanding hydrogel was determined separately to be larger than that needed to rupture typical cells. This physical approach is conceptually simple, technically easy to implement, and potentially generalizable for rupturing a wide range of cells.
author2 School of Chemical and Biomedical Engineering
author_facet School of Chemical and Biomedical Engineering
Fang, Yan
Tan, Jiajun
Lim, Sierin
Soh, Siowling
format Article
author Fang, Yan
Tan, Jiajun
Lim, Sierin
Soh, Siowling
author_sort Fang, Yan
title Rupturing cancer cells by the expansion of functionalized stimuli-responsive hydrogels
title_short Rupturing cancer cells by the expansion of functionalized stimuli-responsive hydrogels
title_full Rupturing cancer cells by the expansion of functionalized stimuli-responsive hydrogels
title_fullStr Rupturing cancer cells by the expansion of functionalized stimuli-responsive hydrogels
title_full_unstemmed Rupturing cancer cells by the expansion of functionalized stimuli-responsive hydrogels
title_sort rupturing cancer cells by the expansion of functionalized stimuli-responsive hydrogels
publishDate 2018
url https://hdl.handle.net/10356/86288
http://hdl.handle.net/10220/45241
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