Rupturing cancer cells by the expansion of functionalized stimuli-responsive hydrogels
Using particles with different functionalities for treating cancer has many advantages over other methods (for example, better access to remote parts of the body); however, current chemical (for example, chemotherapy) and biological (for example, immunotherapy) methods still face many challenges. He...
Saved in:
Main Authors: | , , , |
---|---|
Other Authors: | |
Format: | Article |
Language: | English |
Published: |
2018
|
Subjects: | |
Online Access: | https://hdl.handle.net/10356/86288 http://hdl.handle.net/10220/45241 |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
Institution: | Nanyang Technological University |
Language: | English |
id |
sg-ntu-dr.10356-86288 |
---|---|
record_format |
dspace |
spelling |
sg-ntu-dr.10356-862882023-12-29T06:53:20Z Rupturing cancer cells by the expansion of functionalized stimuli-responsive hydrogels Fang, Yan Tan, Jiajun Lim, Sierin Soh, Siowling School of Chemical and Biomedical Engineering Hydrogels Cancer Cells Using particles with different functionalities for treating cancer has many advantages over other methods (for example, better access to remote parts of the body); however, current chemical (for example, chemotherapy) and biological (for example, immunotherapy) methods still face many challenges. Here, we describe a fundamentally different approach: using the physical force of an expanding stimuli-responsive hydrogel to rupture cancer cells attached on its surface. Specifically, we coated temperature-responsive hydrogels with a layer of cell-adherent arginine-glycine-aspartate (RGD) peptides. The approach involved first allowing cancer cells to attach onto the surface of the hydrogels, and then applying a change in temperature. As the hydrogel underwent a chemical transformation and expanded due to the stimulus, the cancer cells attached to it ruptured. The results from staining the cells with trypan blue, observing them using SEM, and analyzing them using the MTT assay showed that both breast and lung cancer cells died after the hydrogel expanded; hence, we showed that this physical force from the expanding hydrogel is strong enough to rupture the cancer cells. In addition, the force derived from the expanding hydrogel was determined separately to be larger than that needed to rupture typical cells. This physical approach is conceptually simple, technically easy to implement, and potentially generalizable for rupturing a wide range of cells. MOE (Min. of Education, S’pore) Published version 2018-07-26T01:50:28Z 2019-12-06T16:19:41Z 2018-07-26T01:50:28Z 2019-12-06T16:19:41Z 2018 Journal Article Fang, Y., Tan, J., Lim, S., & Soh, S. (2018). Rupturing cancer cells by the expansion of functionalized stimuli-responsive hydrogels. NPG Asia Materials, 10(2), e465-. https://hdl.handle.net/10356/86288 http://hdl.handle.net/10220/45241 10.1038/am.2017.232 en NPG Asia Materials © 2018 The Author(s). This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ 9 p. application/pdf |
institution |
Nanyang Technological University |
building |
NTU Library |
continent |
Asia |
country |
Singapore Singapore |
content_provider |
NTU Library |
collection |
DR-NTU |
language |
English |
topic |
Hydrogels Cancer Cells |
spellingShingle |
Hydrogels Cancer Cells Fang, Yan Tan, Jiajun Lim, Sierin Soh, Siowling Rupturing cancer cells by the expansion of functionalized stimuli-responsive hydrogels |
description |
Using particles with different functionalities for treating cancer has many advantages over other methods (for example, better access to remote parts of the body); however, current chemical (for example, chemotherapy) and biological (for example, immunotherapy) methods still face many challenges. Here, we describe a fundamentally different approach: using the physical force of an expanding stimuli-responsive hydrogel to rupture cancer cells attached on its surface. Specifically, we coated temperature-responsive hydrogels with a layer of cell-adherent arginine-glycine-aspartate (RGD) peptides. The approach involved first allowing cancer cells to attach onto the surface of the hydrogels, and then applying a change in temperature. As the hydrogel underwent a chemical transformation and expanded due to the stimulus, the cancer cells attached to it ruptured. The results from staining the cells with trypan blue, observing them using SEM, and analyzing them using the MTT assay showed that both breast and lung cancer cells died after the hydrogel expanded; hence, we showed that this physical force from the expanding hydrogel is strong enough to rupture the cancer cells. In addition, the force derived from the expanding hydrogel was determined separately to be larger than that needed to rupture typical cells. This physical approach is conceptually simple, technically easy to implement, and potentially generalizable for rupturing a wide range of cells. |
author2 |
School of Chemical and Biomedical Engineering |
author_facet |
School of Chemical and Biomedical Engineering Fang, Yan Tan, Jiajun Lim, Sierin Soh, Siowling |
format |
Article |
author |
Fang, Yan Tan, Jiajun Lim, Sierin Soh, Siowling |
author_sort |
Fang, Yan |
title |
Rupturing cancer cells by the expansion of functionalized stimuli-responsive hydrogels |
title_short |
Rupturing cancer cells by the expansion of functionalized stimuli-responsive hydrogels |
title_full |
Rupturing cancer cells by the expansion of functionalized stimuli-responsive hydrogels |
title_fullStr |
Rupturing cancer cells by the expansion of functionalized stimuli-responsive hydrogels |
title_full_unstemmed |
Rupturing cancer cells by the expansion of functionalized stimuli-responsive hydrogels |
title_sort |
rupturing cancer cells by the expansion of functionalized stimuli-responsive hydrogels |
publishDate |
2018 |
url |
https://hdl.handle.net/10356/86288 http://hdl.handle.net/10220/45241 |
_version_ |
1787136795003060224 |