Protein engineering for covalent immobilization and enhanced stability through incorporation of multiple noncanonical amino acids

• Main Authors: Deepankumar, Kanagavel, Prabhu, Nadarajan Saravanan, Kim, June-Hyung, Yun, Hyungdon
• Other Authors: School of Materials Science & Engineering
• Format: Article
• Language: English
• Published: 2017
• Subjects: Multiple noncanonical amino acids; Fluoroproline
• Online Access: https://hdl.handle.net/10356/86561; http://hdl.handle.net/10220/44074
• Institution: Nanyang Technological University

In this study, we demonstrate the application of multiple functional properties of proteins generated through coupling of residue-specific and site-specific incorporation method. With green fluorescent protein (GFP) as a model protein, we constructed multifunctional GFP through sitespecific incorpor...