Probing Spatial Proximity of Supported Lipid Bilayers to Silica Surfaces by Localized Surface Plasmon Resonance Sensing
On account of high surface sensitivity, localized surface plasmon resonance (LSPR) sensors have proven widely useful for studying lipid membrane configurations at solid–liquid interfaces. Key measurement capabilities include distinguishing adsorbed vesicles from supported lipid bilayers (SLBs) as we...
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sg-ntu-dr.10356-866702023-07-14T15:51:45Z Probing Spatial Proximity of Supported Lipid Bilayers to Silica Surfaces by Localized Surface Plasmon Resonance Sensing Ferhan, Abdul Rahim Jackman, Joshua A. Cho, Nam-Joon School of Chemical and Biomedical Engineering School of Materials Science & Engineering Silica Localized Surface Plasmon Resonance (LSPR) On account of high surface sensitivity, localized surface plasmon resonance (LSPR) sensors have proven widely useful for studying lipid membrane configurations at solid–liquid interfaces. Key measurement capabilities include distinguishing adsorbed vesicles from supported lipid bilayers (SLBs) as well as profiling the extent of deformation among adsorbed vesicles. Such capabilities rely on detecting geometrical changes in lipid membrane configuration on a length scale that is comparable to the decay length of the LSPR-induced electromagnetic field enhancement (∼5–20 nm). Herein, we report that LSPR sensors are also capable of probing nanoscale (∼1 nm) variations in the distance between SLBs and underlying silica-coated surfaces. By tuning the electrostatic properties of lipid membranes, we could modulate the bilayer–substrate interaction and corresponding separation distance, as verified by simultaneous LSPR and quartz crystal microbalance-dissipation (QCM-D) measurements. Theoretical calculations of the expected variation in the LSPR measurement response agree well with experimental results and support that the LSPR measurement response is sensitive to subtle variations in the bilayer–substrate separation distance. NRF (Natl Research Foundation, S’pore) Accepted version 2017-12-12T05:50:21Z 2019-12-06T16:26:59Z 2017-12-12T05:50:21Z 2019-12-06T16:26:59Z 2017 Journal Article Ferhan, A. R., Jackman, J. A., & Cho, N.-J. (2017). Probing Spatial Proximity of Supported Lipid Bilayers to Silica Surfaces by Localized Surface Plasmon Resonance Sensing. Analytical Chemistry, 89(7), 4301-4308. 0003-2700 https://hdl.handle.net/10356/86670 http://hdl.handle.net/10220/44131 10.1021/acs.analchem.7b00370 en Analytical Chemistry © 2017 American Chemical Society (ACS). This is the author created version of a work that has been peer reviewed and accepted for publication by Analytical Chemistry, ACS Publications. It incorporates referee’s comments but changes resulting from the publishing process, such as copyediting, structural formatting, may not be reflected in this document. The published version is available at: [http://dx.doi.org/10.1021/acs.analchem.7b00370]. 17 p. application/pdf |
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Silica Localized Surface Plasmon Resonance (LSPR) Ferhan, Abdul Rahim Jackman, Joshua A. Cho, Nam-Joon Probing Spatial Proximity of Supported Lipid Bilayers to Silica Surfaces by Localized Surface Plasmon Resonance Sensing |
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On account of high surface sensitivity, localized surface plasmon resonance (LSPR) sensors have proven widely useful for studying lipid membrane configurations at solid–liquid interfaces. Key measurement capabilities include distinguishing adsorbed vesicles from supported lipid bilayers (SLBs) as well as profiling the extent of deformation among adsorbed vesicles. Such capabilities rely on detecting geometrical changes in lipid membrane configuration on a length scale that is comparable to the decay length of the LSPR-induced electromagnetic field enhancement (∼5–20 nm). Herein, we report that LSPR sensors are also capable of probing nanoscale (∼1 nm) variations in the distance between SLBs and underlying silica-coated surfaces. By tuning the electrostatic properties of lipid membranes, we could modulate the bilayer–substrate interaction and corresponding separation distance, as verified by simultaneous LSPR and quartz crystal microbalance-dissipation (QCM-D) measurements. Theoretical calculations of the expected variation in the LSPR measurement response agree well with experimental results and support that the LSPR measurement response is sensitive to subtle variations in the bilayer–substrate separation distance. |
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School of Chemical and Biomedical Engineering |
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School of Chemical and Biomedical Engineering Ferhan, Abdul Rahim Jackman, Joshua A. Cho, Nam-Joon |
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Article |
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Ferhan, Abdul Rahim Jackman, Joshua A. Cho, Nam-Joon |
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Ferhan, Abdul Rahim |
title |
Probing Spatial Proximity of Supported Lipid Bilayers to Silica Surfaces by Localized Surface Plasmon Resonance Sensing |
title_short |
Probing Spatial Proximity of Supported Lipid Bilayers to Silica Surfaces by Localized Surface Plasmon Resonance Sensing |
title_full |
Probing Spatial Proximity of Supported Lipid Bilayers to Silica Surfaces by Localized Surface Plasmon Resonance Sensing |
title_fullStr |
Probing Spatial Proximity of Supported Lipid Bilayers to Silica Surfaces by Localized Surface Plasmon Resonance Sensing |
title_full_unstemmed |
Probing Spatial Proximity of Supported Lipid Bilayers to Silica Surfaces by Localized Surface Plasmon Resonance Sensing |
title_sort |
probing spatial proximity of supported lipid bilayers to silica surfaces by localized surface plasmon resonance sensing |
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2017 |
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https://hdl.handle.net/10356/86670 http://hdl.handle.net/10220/44131 |
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1772827802644512768 |