Microbial removal of carboxylic acids from 1,3-propanediol in glycerol anaerobic digestion effluent by PHAs-producing consortium

Anaerobic fermentation of glycerol to 1,3-propanediol (1,3-PDO) is conceived as an economic feasible pathway to handle with increasing crude glycerol from biodiesel industry. However, glycerol anaerobic digestion effluent (ADE) consists of carboxylic acids and 1,3-PDO, imposing difficulties for sepa...

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Main Authors: Pan, Chaozhi, Tan, Giin-Yu Amy, Ge, Liya, Chen, Chia-Lung, Wang, Jing-Yuan
Other Authors: School of Civil and Environmental Engineering
Format: Article
Language:English
Published: 2017
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Online Access:https://hdl.handle.net/10356/86687
http://hdl.handle.net/10220/44151
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Institution: Nanyang Technological University
Language: English
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spelling sg-ntu-dr.10356-866872020-03-07T11:43:36Z Microbial removal of carboxylic acids from 1,3-propanediol in glycerol anaerobic digestion effluent by PHAs-producing consortium Pan, Chaozhi Tan, Giin-Yu Amy Ge, Liya Chen, Chia-Lung Wang, Jing-Yuan School of Civil and Environmental Engineering Nanyang Environment and Water Research Institute Residues and Resource Reclamation Centre Glycerol Anaerobic Digestion Effluent Polyhydroxyalkanoates Anaerobic fermentation of glycerol to 1,3-propanediol (1,3-PDO) is conceived as an economic feasible pathway to handle with increasing crude glycerol from biodiesel industry. However, glycerol anaerobic digestion effluent (ADE) consists of carboxylic acids and 1,3-PDO, imposing difficulties for separation. The objective of this study was, therefore, to investigate microbial removal of carboxylic acids from 1,3-PDO in glycerol ADE by polyhydroxyalkanoates (PHAs) producing consortium. Growth tests on carbon sources showed Corynebacterium hydrocarbooxydans had preference for butyrate while Bacillus megaterium for acetate and glycerol. Consequently, their consortium had a higher cell density and a faster substrate utilization rate than single strain grown in glycerol ADE. Acidic pH at 6.0 and 5.2 strongly inhibited cell growth and activity, while C:N ratio (w/w) at 8:1 could balance nitrogen demand for cell growth and PHA synthesis. Kinetic study further revealed over 80% of fed 1,3-PDO was preserved after depletion of carboxylic acids. Correspondingly, total organic carbon (TOC) contribution from 1,3-PDO rose from initial 55.8% to 84%. Produced PHAs comprised 3-hydroxybutyrate (3-HB) units. The results showed this study as the first attempt to provide a win-win solution to remove carboxylic acids from 1,3-PDO in glycerol ADE and converted them into PHAs as a secondary value-added product. Accepted version 2017-12-15T06:36:48Z 2019-12-06T16:27:21Z 2017-12-15T06:36:48Z 2019-12-06T16:27:21Z 2016 Journal Article Pan, C., Tan, G.-Y. A., Ge, L., Chen, C.-L., & Wang, J.-Y. (2016). Microbial removal of carboxylic acids from 1,3-propanediol in glycerol anaerobic digestion effluent by PHAs-producing consortium. Biochemical Engineering Journal, 112, 269-276. 1369-703X https://hdl.handle.net/10356/86687 http://hdl.handle.net/10220/44151 10.1016/j.bej.2016.04.031 en Biochemical Engineering Journal © 2016 Elsevier. This is the author created version of a work that has been peer reviewed and accepted for publication by Biochemical Engineering Journal, Elsevier. It incorporates referee’s comments but changes resulting from the publishing process, such as copyediting, structural formatting, may not be reflected in this document. The published version is available at: [http://dx.doi.org/10.1016/j.bej.2016.04.031]. 34 p. application/pdf
institution Nanyang Technological University
building NTU Library
country Singapore
collection DR-NTU
language English
topic Glycerol Anaerobic Digestion Effluent
Polyhydroxyalkanoates
spellingShingle Glycerol Anaerobic Digestion Effluent
Polyhydroxyalkanoates
Pan, Chaozhi
Tan, Giin-Yu Amy
Ge, Liya
Chen, Chia-Lung
Wang, Jing-Yuan
Microbial removal of carboxylic acids from 1,3-propanediol in glycerol anaerobic digestion effluent by PHAs-producing consortium
description Anaerobic fermentation of glycerol to 1,3-propanediol (1,3-PDO) is conceived as an economic feasible pathway to handle with increasing crude glycerol from biodiesel industry. However, glycerol anaerobic digestion effluent (ADE) consists of carboxylic acids and 1,3-PDO, imposing difficulties for separation. The objective of this study was, therefore, to investigate microbial removal of carboxylic acids from 1,3-PDO in glycerol ADE by polyhydroxyalkanoates (PHAs) producing consortium. Growth tests on carbon sources showed Corynebacterium hydrocarbooxydans had preference for butyrate while Bacillus megaterium for acetate and glycerol. Consequently, their consortium had a higher cell density and a faster substrate utilization rate than single strain grown in glycerol ADE. Acidic pH at 6.0 and 5.2 strongly inhibited cell growth and activity, while C:N ratio (w/w) at 8:1 could balance nitrogen demand for cell growth and PHA synthesis. Kinetic study further revealed over 80% of fed 1,3-PDO was preserved after depletion of carboxylic acids. Correspondingly, total organic carbon (TOC) contribution from 1,3-PDO rose from initial 55.8% to 84%. Produced PHAs comprised 3-hydroxybutyrate (3-HB) units. The results showed this study as the first attempt to provide a win-win solution to remove carboxylic acids from 1,3-PDO in glycerol ADE and converted them into PHAs as a secondary value-added product.
author2 School of Civil and Environmental Engineering
author_facet School of Civil and Environmental Engineering
Pan, Chaozhi
Tan, Giin-Yu Amy
Ge, Liya
Chen, Chia-Lung
Wang, Jing-Yuan
format Article
author Pan, Chaozhi
Tan, Giin-Yu Amy
Ge, Liya
Chen, Chia-Lung
Wang, Jing-Yuan
author_sort Pan, Chaozhi
title Microbial removal of carboxylic acids from 1,3-propanediol in glycerol anaerobic digestion effluent by PHAs-producing consortium
title_short Microbial removal of carboxylic acids from 1,3-propanediol in glycerol anaerobic digestion effluent by PHAs-producing consortium
title_full Microbial removal of carboxylic acids from 1,3-propanediol in glycerol anaerobic digestion effluent by PHAs-producing consortium
title_fullStr Microbial removal of carboxylic acids from 1,3-propanediol in glycerol anaerobic digestion effluent by PHAs-producing consortium
title_full_unstemmed Microbial removal of carboxylic acids from 1,3-propanediol in glycerol anaerobic digestion effluent by PHAs-producing consortium
title_sort microbial removal of carboxylic acids from 1,3-propanediol in glycerol anaerobic digestion effluent by phas-producing consortium
publishDate 2017
url https://hdl.handle.net/10356/86687
http://hdl.handle.net/10220/44151
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