Progress and Challenges towards Point-of-Care Diagnostic Development for Dengue

• Main Authors: Pang, Junxiong, Chia, Po Ying, Lye, David Chienboon, Leo, Yee Sin
• Other Authors: Kraft, Colleen Suzanne
• Format: Article
• Language: English
• Published: 2018
• Subjects: Clinical Diagnosis; Biotechnology
• Online Access: https://hdl.handle.net/10356/87254; http://hdl.handle.net/10220/44374
• Institution: Nanyang Technological University

Dengue detection strategies involve viral RNA, antigen, and/or antibody detection. Each strategy has its advantages and disadvantages. Optimal, user-friendly, rapid diagnostic tests based on immunochromatographic assays are pragmatic point-of-care tests (POCTs) in regions where dengue is endemic where there are limited laboratory capabilities and optimal storage conditions. Increasingly, there is a greater public health significance for a multiplexing assay that differentiates dengue from Zika or pathogens with similar clinical presentations. Although there have been many assay/platform developments toward POCTs, independent validation and implementation remain very limited. This review highlights the current key progress and challenges toward the development of a dengue POCT.