Resonance raman probes for organelle-specific labeling in live cells

Raman microspectroscopy provides for high-resolution non-invasive molecular analysis of biological samples and has a breakthrough potential for dissection of cellular molecular composition at a single organelle level. However, the potential of Raman microspectroscopy can be fully realized only when...

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Main Authors: Kuzmin, Andrey N., Pliss, Artem, Lim, Chang-Keun, Heo, Jeongyun, Kim, Sehoon, Rzhevskii, Alexander, Gu, Bobo, Yong, Ken-Tye, Wen, Shuangchun, Prasad, Paras N.
Other Authors: School of Electrical and Electronic Engineering
Format: Article
Language:English
Published: 2018
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Online Access:https://hdl.handle.net/10356/87490
http://hdl.handle.net/10220/46745
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Institution: Nanyang Technological University
Language: English
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spelling sg-ntu-dr.10356-874902022-02-16T16:31:04Z Resonance raman probes for organelle-specific labeling in live cells Kuzmin, Andrey N. Pliss, Artem Lim, Chang-Keun Heo, Jeongyun Kim, Sehoon Rzhevskii, Alexander Gu, Bobo Yong, Ken-Tye Wen, Shuangchun Prasad, Paras N. School of Electrical and Electronic Engineering Raman Spectrometry Molecular Probes DRNTU::Engineering::Electrical and electronic engineering Raman microspectroscopy provides for high-resolution non-invasive molecular analysis of biological samples and has a breakthrough potential for dissection of cellular molecular composition at a single organelle level. However, the potential of Raman microspectroscopy can be fully realized only when novel types of molecular probes distinguishable in the Raman spectroscopy modality are developed for labeling of specific cellular domains to guide spectrochemical spatial imaging. Here we report on the design of a next generation Raman probe, based on BlackBerry Quencher 650 compound, which provides unprecedentedly high signal intensity through the Resonance Raman (RR) enhancement mechanism. Remarkably, RR enhancement occurs with low-toxic red light, which is close to maximum transparency in the biological optical window. The utility of proposed RR probes was validated for targeting lysosomes in live cultured cells, which enabled identification and subsequent monitoring of dynamic changes in this organelle by Raman imaging. Published version 2018-11-30T02:23:37Z 2019-12-06T16:43:00Z 2018-11-30T02:23:37Z 2019-12-06T16:43:00Z 2016 Journal Article Kuzmin, A. N., Pliss, A., Lim, C. K., Heo, J., Kim, S., Rzhevskii, A., . . . Prasad, P. N. (2016). Resonance raman probes for organelle-specific labeling in live cells. Scientific Reports, 6, 28483-. doi:10.1038/srep28483 https://hdl.handle.net/10356/87490 http://hdl.handle.net/10220/46745 10.1038/srep28483 27339882 en Scientific Reports © 2016 The Authors (Nature Publishing Group). This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ 9 p. application/pdf
institution Nanyang Technological University
building NTU Library
continent Asia
country Singapore
Singapore
content_provider NTU Library
collection DR-NTU
language English
topic Raman Spectrometry
Molecular Probes
DRNTU::Engineering::Electrical and electronic engineering
spellingShingle Raman Spectrometry
Molecular Probes
DRNTU::Engineering::Electrical and electronic engineering
Kuzmin, Andrey N.
Pliss, Artem
Lim, Chang-Keun
Heo, Jeongyun
Kim, Sehoon
Rzhevskii, Alexander
Gu, Bobo
Yong, Ken-Tye
Wen, Shuangchun
Prasad, Paras N.
Resonance raman probes for organelle-specific labeling in live cells
description Raman microspectroscopy provides for high-resolution non-invasive molecular analysis of biological samples and has a breakthrough potential for dissection of cellular molecular composition at a single organelle level. However, the potential of Raman microspectroscopy can be fully realized only when novel types of molecular probes distinguishable in the Raman spectroscopy modality are developed for labeling of specific cellular domains to guide spectrochemical spatial imaging. Here we report on the design of a next generation Raman probe, based on BlackBerry Quencher 650 compound, which provides unprecedentedly high signal intensity through the Resonance Raman (RR) enhancement mechanism. Remarkably, RR enhancement occurs with low-toxic red light, which is close to maximum transparency in the biological optical window. The utility of proposed RR probes was validated for targeting lysosomes in live cultured cells, which enabled identification and subsequent monitoring of dynamic changes in this organelle by Raman imaging.
author2 School of Electrical and Electronic Engineering
author_facet School of Electrical and Electronic Engineering
Kuzmin, Andrey N.
Pliss, Artem
Lim, Chang-Keun
Heo, Jeongyun
Kim, Sehoon
Rzhevskii, Alexander
Gu, Bobo
Yong, Ken-Tye
Wen, Shuangchun
Prasad, Paras N.
format Article
author Kuzmin, Andrey N.
Pliss, Artem
Lim, Chang-Keun
Heo, Jeongyun
Kim, Sehoon
Rzhevskii, Alexander
Gu, Bobo
Yong, Ken-Tye
Wen, Shuangchun
Prasad, Paras N.
author_sort Kuzmin, Andrey N.
title Resonance raman probes for organelle-specific labeling in live cells
title_short Resonance raman probes for organelle-specific labeling in live cells
title_full Resonance raman probes for organelle-specific labeling in live cells
title_fullStr Resonance raman probes for organelle-specific labeling in live cells
title_full_unstemmed Resonance raman probes for organelle-specific labeling in live cells
title_sort resonance raman probes for organelle-specific labeling in live cells
publishDate 2018
url https://hdl.handle.net/10356/87490
http://hdl.handle.net/10220/46745
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